Mutation
Kinetic parameters
Tree Display
AceDB Schema
XML Display
Feedback

Kinetic parameters for mutation K285D_bunfa-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 58.7 & 4.1 uM - T25C IS 50 mM phosphate Cousin_1996_J.Biol.Chem_271_15099

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 10.8 & 2.4 nM 14.4 & 0.8 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
D-tubocurarine 8.6 & 1.5 uM 17.7 & 3.3 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Decamethonium 2.3 & 0.3 uM 14.1 & 3.9 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Edrophonium 0.6 &0.0 uM 10.4 & 2.5 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Gallamine~Flaxedil 26.1 & 2.9 uM - - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Propidium 4.6 & 0.8 uM 27.9 & 5.0 uM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
WT-fasciculin2 - 3.6 & 1.3 nM - T25C IS 50 mM phosphate Acetylthiocholine Cousin_1996_J.Biol.Chem_271_15099
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer