Mutation

Kinetic parameters

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Kinetic parameters for mutation G117E_human-BCHE

Substrate

MoleculeKmKcatExp. conditionsReference
Butyrylthiocholine 1.5 & 0.3 mM 17000 & 2000 /min 50mM NaPhosphate pH7.4 Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 330 & 140 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
Cocaine 8700 & 5300 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
Dibucaine 19 & 3 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
Solanine 12.3 & 9.9 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
Succinylcholine~Suxamethonium 90 & 50 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423
Tacrine 0.2 & 0.2 uM - - 50mM NaPhosphate pH7.4 Butyrylthiocholine Loewenstein-Lichtenstein_1996_Mol.Pharmacol_50_1423

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Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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