Mutation

Kinetic parameters

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Kinetic parameters for mutation F295L/F297V_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 1300 uM 0.5 10+5/min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433
Butyrylthiocholine 0.42 mM 0.43 10+5/min IS 50mM phosphate pH8 T27C Kaplan_2001_Biochemistry_40_7433

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
Decamethonium 210 uM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
Edrophonium 2.2 uM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
HuperzineA 50 nM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433
Tacrine 60 nM - - T25C IS 50 mM phosphate Acetylthiocholine Kaplan_2001_Biochemistry_40_7433

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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