Kinetic parameters for mutation F295A_human-ACHE

Molecule	Km	Kcat	Exp. conditions	Reference
Acetylthiocholine	130 uM	300000/min	50mM Na phosphate pH8	Barak_1994_J.Biol.Chem_269_6296

Inhibitor

Molecule	Ki	Ksi	Ic50	Exp. conditions	Substrate	Reference
BW284C51	3.5 nM	-	-	50mM Na phosphate pH8	Acetylthiocholine	Barak_1994_J.Biol.Chem_269_6296
Decamethonium	0.8 uM	-	-	50mM Na phosphate pH8	Acetylthiocholine	Barak_1994_J.Biol.Chem_269_6296
Edrophonium	0.4 uM	-	-	50mM Na phosphate pH8	Acetylthiocholine	Barak_1994_J.Biol.Chem_269_6296
Hexamethonium	0.3 mM	-	-	50mM Na phosphate pH8	Acetylthiocholine	Barak_1994_J.Biol.Chem_269_6296
HuperzineA	205 nM	-	-	50mM Na phosphate pH8 T22C	Acetylthiocholine	Ariel_1998_Biochem.J_335_95
Propidium	0.9 uM	-	-	50mM Na phosphate pH8	Acetylthiocholine	Barak_1994_J.Biol.Chem_269_6296
Tacrine	60 & 8 nM	30 & 10 nM	-	50mM Na phosphate pH8 T22C	Acetylthiocholine	Ariel_1998_Biochem.J_335_95

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page