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Kinetic parameters for mutation E84Q_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
3,3-dimethylbutylthioacetate 300 & 60 uM 50000 & 10000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
3,3-dimethylbutylthioacetate 230 & 20 uM 43000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
Acetylthiocholine 270 & 30 uM 400000 & 60000/min 50mM NaPhosphate pH8 Kronman_1994_J.Biol.Chem_269_27819
Acetylthiocholine 150 & 20 uM 400000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
Edrophonium 1.0 & 0.1 uM - - 50mM NaPhosphate pH8 3.3-dimethylbutylthioacetate Kronman_1994_J.Biol.Chem_269_27819
Edrophonium 1.2 & 0.1 uM - - 50mM NaPhosphate pH8 Acetylthiocholine Kronman_1994_J.Biol.Chem_269_27819
Edrophonium 1.20 & 0.20 uM - - IS 150mM Acetylthiocholine Shafferman_1994_EMBO.J_13_3448
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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