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Kinetic parameters for mutation E285A_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
3,3-dimethylbutylthioacetate 230 & 30 uM 42000 & 6000/min IS 5mM Shafferman_1994_EMBO.J_13_3448
Acetylthiocholine 280 uM 380000/min 50mM Na phosphate pH8 Barak_1994_J.Biol.Chem_269_6296
Acetylthiocholine 170 & 30 uM 400000 & 60000/min IS 5mM Shafferman_1994_EMBO.J_13_3448

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 21 nM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Decamethonium 26 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Edrophonium 0.8 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Edrophonium 1.00 & 0.15 uM - - IS 150mM Acetylthiocholine Shafferman_1994_EMBO.J_13_3448
Hexamethonium 1.3 mM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Propidium 6.5 uM - - 50mM Na phosphate pH8 Acetylthiocholine Barak_1994_J.Biol.Chem_269_6296
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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