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Kinetic parameters for mutation E202A_human-ACHE

Substrate

MoleculeKmKcatExp. conditionsReference
Acetylthiocholine 660 & 10 uM 10000 & 2000/min pH8 T27C 50mM Na phosphate Shafferman_1992_EMBO.J_11_3561

Inhibitor

MoleculeKiKsiIc50Exp. conditionsSubstrateReference
BW284C51 - - 40000 nM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Decamethonium - - 654 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Edrophonium - - 12 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
HuperzineA 1600 & 300 nM - - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95
Propidium - - 1.8 uM pH8 T27C 50mM Na phosphate Acetylthiocholine Shafferman_1992_EMBO.J_11_3561
Tacrine 3300 & 150 nM 16000 & 3000 nM - 50mM Na phosphate pH8 T22C Acetylthiocholine Ariel_1998_Biochem.J_335_95
Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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