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Kinetic parameters for mutation B5-174-A175-575_mouse-chimerae-ACHE-BCHE
Substrate
Molecule
Km
Kcat
Exp. conditions
Reference
Acetylthiocholine
160 & 2 uM
170000 & 20000/min
100mM NaP pH7 T22C eq3 sch3
Radic_1993_Biochemistry_32_12074
Inhibitor
Molecule
Ki
Ksi
Ic50
Exp. conditions
Substrate
Reference
WT-fasciculin2
-
91000 & 16000 pM
-
100mM NaP pH7.0 T37C eq4 sch4
Acetylthiocholine
Radic_1994_J.Biol.Chem_269_11233
Send your questions or comments to :
Mail to:
Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite:
Lenfant 2013 Nucleic.Acids.Res.
or
Marchot Chatonnet 2012 Prot.Pept Lett.
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