Gene_locus Report for: ustma-q4pep1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) Ustilago maydis lipase 2 (Uml2)
Comment
Uml2 release short- and long-chain fatty acids from p-nitrophenyl esters. Tween 20/80 and phosphatidylcholine containing long-chain saturated or unsaturated fatty acids are also substrates Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Basidiomycota: N E > Ustilaginomycotina: N E > Ustilaginomycetes: N E > Ustilaginales: N E > Ustilaginaceae: N E > Ustilago: N E > Ustilago maydis: N E > Ustilago maydis 521: N E 6_AlphaBeta_hydrolase :
ustma-q4p1m4 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p2u2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p544 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pbl1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4phd7 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pi12 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
A85-EsteraseD-FGH :
ustma-q4p633 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
ABHD11-Acetyl_transferase :
ustma-q4pdj1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
ABHD13-BEM46 :
ustma-q4p5b4 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Acidic_Lipase :
ustma-q4p139 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
AlphaBeta_hydrolase :
ustma-q4pd13 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pd19 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Carboxypeptidase_S10 :
ustma-q4p5h2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p7d8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p7g6 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p8u8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pdc5 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pdc7 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
CFTR-inhibitory-factor_Cif :
ustma-q4p052 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
CGI-58_ABHD5_ABHD4 :
ustma-q4p0v2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Cocaine_esterase :
ustma-q4p906 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Cutinase :
ustma-q4p8k0 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p198 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pbk7 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4phg8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Dienelactone_hydrolase :
ustma-q4pbi1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pdv1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
DPP4N_Peptidase_S9 :
ustma-q4p3p0 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Duf_676 :
ustma-q4p8v8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Duf_1100-S :
ustma-q4pcx8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Epoxide_hydrolase :
ustma-q4p6v2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p750 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pd75 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pdg1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
FSH1 :
ustma-q4p0t2 Ustilago maydis (Corn smut fungus); Sporisorium reilianum (Maize head smut fungus)); Ustilago hordei; Moesziomyces Pseudozyma antarctica; Pseudozyma hubeiensis; Kalmanozyma brasiliensis; Moesziomyces Pseudozyma antarctica; Sporisorium scitamineum; Sporisorium reilianum; Sporisorium graminicola, hypothetical protein .
Fungal-Bact_LIP :
ustma-q4p903 ,
ustma-q4pe08 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Fungal_carboxylesterase_lipase :
ustma-A0A0D1BV83 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-A0A0D1BW24 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-A0A0D1DYF7 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-A0A0D1DTW1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-A0A0D1C5D2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-A0A0D1E8B5 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Homoserine_transacetylase :
ustma-q4p1g6 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p8y8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Hormone-sensitive_lipase_like :
ustma-q4p6b4 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p9t5 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p075 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p081 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p414 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p742 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pbj2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pg61 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pgx6 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Lipase_3 :
ustma-q4pbm4 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pcp7 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pcq2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pdu2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein (c-term 550->) ,
ustma-q4phr5 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4phz2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
LYsophospholipase_carboxylesterase :
ustma-apth1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4p3f4 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Monoglyceridelipase_lysophospholip :
ustma-q4pih8 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) Sporisorium reilianum (strain SRZ2) (Maize head smut fungus) hypothetical protein .
PAF-Acetylhydrolase :
ustma-q4pd86 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein ,
ustma-q4pid0 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Palmitoyl-protein_thioesterase :
ustma-q4p2m2 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
PC-sterol_acyltransferase :
ustma-q4phu1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
PGAP1 :
ustma-q4p782 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
Proline_iminopeptidase :
ustma-q4phi1 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein .
S9N_PPCE_Peptidase_S9 :
ustma-q4p3m5 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) Prolyl endopeptidase .
Thioesterase :
ustma-q4p0e9 Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) hypothetical protein Molecular evidence
Database
Sequence
Graphical view for this peptide sequence: ustma-q4pep1 Colored MSA for Canar_LipB (raw)
MKTTSVISALVTLASIIRAAPLASSDPAFSTPKATLDAGLECQTGSPSSQ
TKPILLVPGTGANGTQTFDSSWIPLSAKLGFSPCWISPPPFMLNDSQVNV
EYIVNAVQTLYAGSGSKKVPVLTWSQGGLATQWALTFFPSIRNQVDRLMA
FAPDYKGTIEAGLLSTFGLASQSVWQQQAGSAFVTALKNAGGLTSFVPTT
NLYSFFDEIVQPQVFNSDADSSYLGNSKNIQAQTVCGGFFVIDHAGSLTS
QFSYVVGKSALTSSSGVANSADYSSKDCKASPADDLSAKQKADASALLFV
AAGNLLAGPKQNCEPDLKPYARQFAVGKKTCSGTIN
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M K T T S V I S A L V T L A S I I R A A P L A S S D P A F S T P K A T L D A G L E C Q T G S P S S Q T K P I L L V P G T G A N G T Q T F D S S W I P L S A K L G F S P C W I S P P P F M L N D S Q V N V E Y I V N A V Q T L Y A G S G S K K V P V L T W S Q G G L A T Q W A L T F F P S I R N Q V D R L M A F A P D Y K G T I E A G L L S T F G L A S Q S V W Q Q Q A G S A F V T A L K N A G G L T S F V P T T N L Y S F F D E I V Q P Q V F N S D A D S S Y L G N S K N I Q A Q T V C G G F F V I D H A G S L T S Q F S Y V V G K S A L T S S S G V A N S A D Y S S K D C K A S P A D D L S A K Q K A D A S A L L F V A A G N L L A G P K Q N C E P D L K P Y A R Q F A V G K K T C S G T I N
Reference
Title: Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activityBuerth C , Kovacic F , Stock J , Terfruchte M , Wilhelm S , Jaeger KE , Feldbrugge M , Schipper K , Ernst JF , Tielker D Ref: Applied Microbiology & Biotechnology, 98 :4963, 2014 : PubMed Abstract ESTHER: Buerth_2014_Appl.Microbiol.Biotechnol_98_4963 PubMedSearch: Buerth 2014 Appl.Microbiol.Biotechnol 98 4963 PubMedID: 24469105 Gene_locus related to this paper: ustma-q4pep1 Abstract
CalB of Pseudozyma aphidis (formerly named Candida antarctica) is one of the most widely applied enzymes in industrial biocatalysis. Here, we describe a protein with 66 % sequence identity to CalB, designated Ustilago maydis lipase 2 (Uml2), which was identified as the product of gene um01422 of the corn smut fungus U. maydis. Sequence analysis of Uml2 revealed the presence of a typical lipase catalytic triad, Ser-His-Asp with Ser125 located in a Thr-Xaa-Ser-Xaa-Gly pentapeptide. Deletion of the uml2 gene in U. maydis diminished the ability of cells to hydrolyse fatty acids from tributyrin or Tween 20/80 substrates, thus demonstrating that Uml2 functions as a lipase that may contribute to nutrition of this fungal pathogen. Uml2 was heterologously produced in Pichia pastoris and recombinant N-glycosylated Uml2 protein was purified from the culture medium. Purified Uml2 released short- and long-chain fatty acids from p-nitrophenyl esters and Tween 20/80 substrates. Furthermore, phosphatidylcholine substrates containing long-chain saturated or unsaturated fatty acids were effectively hydrolysed. Both esterase and phospholipase A activity of Uml2 depended on the Ser125 catalytic residue. These results indicate that Uml2, in contrast to CalB, exhibits not only esterase and lipase activity but also phospholipase A activity. Thus, by genome mining, we identified a novel CalB-like lipase with different substrate specificities.
Other Papers
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) lipase UM03410