(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Crustacea: NE > Maxillopoda: NE > Copepoda: NE > Neocopepoda: NE > Podoplea: NE > Harpacticoida: NE > Harpacticidae: NE > Tigriopus: NE > Tigriopus japonicus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRPKRAKGRLSTSLLLHKVVLTLTVMAVVMLMACCHTATAYISDDLVVET NKGKIRGVTLKSATNKDVDVWYGIPYAQPPLGNLRFRHPRPIDAWSGIKE TTKLPNSCIQIYDTAFPGFYGSEMWNANTPISEDCLYINVAVPKPHPKNS AVLVWVFGGGFYSGSSTLDVYDMRVLASEENIILVSFQYRVASLGFLFFD TEDVPGNAAMFDQIMALQWIKDNIAHFGGNPDNITLFGESAGATSVSLHL LSPLSRNLFSQVIMQSASALVPWGIITKEESLIRGLRLAELMNCPHDRKD LRATIECLRQKNATEMVNKEWDGIVFGICEFPFVPIIDGSFLDETPQTAV NSENFKKTNIMMGTNQDEAFFFIMYYLVDIFKNEESVHVTREDFLRLVSE TNLYVKHVGREAIIYEYTDWLNPNNAIKNRDAIDKMVGDYAFTCPVVEFG YQYASSGNNVYMYYFSERASTNPWPTWAGVLHGDEIAFIFGEPLNRSRNY DQSEIALSRRMMAYWANFAKTGNPSLSADGTWATTYWPRHTADKREVLEL NANYTRTLNGLRVKKCAFWNKFLPKLLSSVESPRPCDKSCCKNMGGEAAS FVGHISIHWIFDR
Reference
Title: Inhibitory effects of biocides on transcription and protein activity of acetylcholinesterase in the intertidal copepod Tigriopus japonicus Lee JW, Kim BM, Jeong CB, Won EJ, Rhee JS, Lee JS Ref: Comparative Biochemistry & Physiology C Pharmacol Toxicol, 167C:147, 2014 : PubMed
Acetlycholinesterase (AChE) is a serine esterase that plays an important role in the hydrolytic degradation of acetylcholine. We investigated the modulatory potential of T. japonicus-AChE (TJ-AChE) for biocide response by cloning, sequencing, and characterizing the full-length genomic DNA of the TJ-AChE1 and TJ-AChE2 genes. The deduced TJ-AChE proteins were highly conserved across species and were distinctively separated into two subtypes, AChE1 and AChE2. Each TJ-AChE protein was closely phylogenetically clustered with invertebrate AChE1 and AChE2 proteins. Transcriptional level of TJ-AChE1 was higher than TJ-AChE2 in all developmental stages. TJ-AChE1 mRNA decreased in response to five biocides (alachlor, chlorpyrifos, dimethoate, endosulfan, lindane,) but not in the molinate-exposed group. TJ-AChE2 decreased significantly only in response to chlorpyrifos and lindane. TJ-AChE enzymatic activity was significantly inhibited when exposed to alachlor, chlorpyrifos, endosulfan, or lindane for 24h. This study elucidates potential endogenous mechanisms of biocide-induced neurotoxicity in T. japonicus.