(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Mammalia: NE > Theria: NE > Eutheria: NE > Boreoeutheria: NE > Laurasiatheria: NE > Carnivora: NE > Feliformia: NE > Felidae: NE > Pantherinae: NE > Panthera: NE > Panthera tigris: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MQSKGTIISIQFLLRFLLLWVLIGKSHTEEDIIITTKNGKVRGMNLPVLD GTVTAFLGIPYAQPPLGRLRFKKPQFLTKWSDIWNATKHANSCYQNADQS FPGFPGSEMWNPNTDLSEDCLYLNVWSPTPKPKNATVMIWIYGGGFQTGT SSLPVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEIPGNMGLFDQQ LALQWVQKNIAAFGGNPKSVTLFGESAGAGSVSLHLLSPRSQPLFTRAIL QSGSSNAPWAVMSLDEAKNRTLTLAKFIGCSKENDTEIIKCLRNKDPQEI LLNELLVVPSDTLLSVNFGPVVDGDFLTDMPDTLLQLGQFKKTQILVGVN KDEGTAFLVYGAPGFSKDNDSIITRKEFQEGLKIYFPGVSEFGREAILFY YVDLLDDQRAEKYREALDDVLGDYNIICPALEFTTKFSELGNNAFFYYFE HRSSQLPWPEWMGVMHGYEIEFVFGLPLERRVNYTRAEEILSRSIMNYWA NFAKYGNPNGTQNNSTRWPAFRSTDQKYLTLNAESPKVYTKLRAQQCRFW TLFFPKVLEMTGNIDEAEREWRAGFYRWNNYMMDWKNQFNDYTSKKESCA GL
Reference
Title: Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma Bartels CF, Xie W, Miller-Lindholm AK, Schopfer LM, Lockridge O Ref: Biochemical Pharmacology, 60:479, 2000 : PubMed
Cat serum contains 0.5 mg/L of butyrylcholinesterase (BChE, EC 3.1.1. 8) and 0.3 mg/L of acetylcholinesterase (AChE, EC 3.1.1.7); this can be compared with 5 mg/mL and < 0.01 mg/L, respectively, in human serum. Cat BChE differed from human BChE in the steady-state turnover of butyrylthiocholine, having a 3-fold higher k(cat) and 2-fold higher K(m) and K(ss) values. Sequencing of the cat BCHE cDNA revealed 70 amino acid differences between cat and human BChE, three of which could account for these kinetic differences. These amino acids, which were located in the region of the active site, were Phe398Ile, Pro285Leu, and Ala277Leu (where the first amino acid was found in human and the second in cat). Sequencing genomic DNA for cat and human ACHE demonstrated that there were 33 amino acid differences between the cat and human AChE enzymes, but that there were no differences in the active site region. In addition, a polymorphism in intron 3 of the human ACHE gene was detected, as well as a silent polymorphism at Y116 of the cat ACHE gene.
