Gene_locus Report for: staha-Q9RGZ6

Rosenstein R, Gotz F a review on Staphylococcal lipases. There is a long precursor which is cut to a 46kDa active form

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Firmicutes: N E > Bacilli: N E > Bacillales: N E > Staphylococcaceae: N E > Staphylococcus: N E > Staphylococcus haemolyticus: N E

Title: Staphylococcus haemolyticus lipase: biochemical properties, substrate specificity and gene cloning
Oh B, Kim H, Lee J, Kang S, Oh T
Ref: FEMS Microbiology Letters, 179:385, 1999 : PubMed
Abstract


ESTHER: Oh_1999_FEMS.Microbiol.Lett_179_385
PubMedSearch: Oh 1999 FEMS.Microbiol.Lett 179 385
PubMedID: 10518741
Gene_locus related to this paper: staha-Q9RGZ6

Abstract

Lipase of Staphylococcus haemolyticus L62 was purified from culture supernatant and its molecular mass was estimated to be 45 kDa by SDS-PAGE. Its optimum temperature and pH for the hydrolysis of olive oil was 28 degrees C and pH 8.5, respectively. The enzyme was stable up to 50 degrees C in the presence of Ca(2+)and over the pH range 5-11. It had high hydrolytic activity against tributyrin, tripropionin, and trimyristin among various triglycerides. The gene encoding the lipase was cloned in Escherichia coli. Sequence analysis showed an open reading frame of 2136 bp, which encodes a preproenzyme of 711 amino acids. The preproenzyme is composed of a signal peptide (60 aa), a pro-peptide (259 aa), and a mature enzyme (392 aa). The mature enzyme has 49-67% amino acid sequence homology with other staphylococcal lipases.