sorbi-hnl
Sorghum bicolor mRNA for p-(S)-hydroxymandelonitrile lyase (SbHNL)
Comment
a deletion of two aa adjacent to active serine is responsible in part of a dramatically different active site and catalytic mechanism compared to wheat carboxypeptidase ( the structure of the cristallized isozyme II has some amino acid differences Leu11 Ala79 Gly112 Ser203 Thr408 Val409 Arg 410)
Relationship
Molecular evidence
Sequence
Peptide
MAVFISSSGS PGRATATTTT TTTLLLAVLA AAAAAGLLLA PVAARGSPPE HDKQLQLQQQ EDDRIPGLPG QPNGVAFGMY GGYVTIDDNN GRALYYWFQE ADTADPAAAP LVLWLNGGPG CSSIGLGAMQ ELGPFRVHTN GESLLLNEYA WNKAANILFA ESPAGVVFSY SNTSSDLSMG DDKMAQDTYT FLVKWFERFP HYNYREFYIA GESGHFIPQL SQVVYRNRNN SPFINFQGLL VSSGLTNDHE DMIGMFELWW HHGLISDETR DSGLKVCPGT SFMHPTPECT EVWNKALAEQ GNINPYTIYT PTCDREPSPY QRRFWAPHGR AAPPPLMLPP YDPCAVFNSI NYLNLPEVQT ALHANVSGIV EYPWTVCSNT IFDQWGQAAD DLLPVYRELI QAGLRVWVYS GDTDSVVPVS STRRSLAALE LPVKTSWYPW YMAPTEREVG GWSVQYEGLT YVSPSGAGHL VPVHRPAQAF LLFKQFLKGE PMPAEEKNDI LLPSEKAPFY
References
| Title : Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme - Lauble_2002_Biochemistry_41_12043 |
| Author(s) : Lauble H , Miehlich B , Forster S , Wajant H , Effenberger F |
| Ref : Biochemistry , 41 :12043 , 2002 |
| Abstract : Lauble_2002_Biochemistry_41_12043 |
| ESTHER : Lauble_2002_Biochemistry_41_12043 |
| PubMedSearch : Lauble_2002_Biochemistry_41_12043 |
| PubMedID: 12356304 |
| Gene_locus related to this paper: sorbi-hnl |
| Title : Molecular cloning of hydroxynitrile lyase from Sorghum bicolor (L.). Homologies to serine carboxypeptidases - Wajant_1994_Plant.Mol.Biol_26_735 |
| Author(s) : Wajant H , Mundry KW , Pfizenmaier K |
| Ref : Plant Mol Biol , 26 :735 , 1994 |
| Abstract : Wajant_1994_Plant.Mol.Biol_26_735 |
| ESTHER : Wajant_1994_Plant.Mol.Biol_26_735 |
| PubMedSearch : Wajant_1994_Plant.Mol.Biol_26_735 |
| PubMedID: 7948927 |
| Gene_locus related to this paper: sorbi-hnl |