(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Mammalia: NE > Theria: NE > Eutheria: NE > Boreoeutheria: NE > Laurasiatheria: NE > Cetartiodactyla: NE > Ruminantia: NE > Pecora: NE > Bovidae: NE > Caprinae: NE > Ovis: NE > Ovis aries: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MQSRSTVIYLRFVLWFLLLWVLFEKSHTEEDIIITTKNGKVRGMRLPVLG GTVTAFLGIPYAQPPLGRLRFKKPQSLTKWPDIWNATKYANSCYQNTDQS FPGFLGSEMWNPNTDLSEDCLYLNVWIPTPKPKNATVMIWIYGGSFQTGT SSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNVGLFDQQ LALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPKSHPLFTRAIL QSGSSNAPWAVTSLYEARNRTLTLAKFIGCSRENDTEIIKCLRNKEPQEI LLHEVFVVPYGTLLSVNFGPTVDGDFLTDMPDTLLQLGQFKKTQILVGVN KDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGRESILFH YMDWLDDQRAENYREALDDVVGDYNIICPALEFTKKFSDMGNNAFFYYFE HRSSKLPWPEWMGVMHGYEIEFVFGLPLERRVNYTKAEEIFSRSIMKRWA NFAKYGNPNGTQNNSTRWPVFKSNEQKYFTLNTESPKVNTKLRAQQCRFW TLFFPKVLEITGNIDEVEREWKAGFHRWNNYMMDWKNQFNDYTSKKESCA GL
Until recently, the construction of a reference genome was performed using Sanger sequencing alone. The emergence of next-generation sequencing platforms now means reference genomes may incorporate sequence data generated from a range of sequencing platforms, each of which have different read length, systematic biases and mate-pair characteristics. The objective of this review is to inform the mammalian genomics community about the experimental strategy being pursued by the International Sheep Genomics Consortium (ISGC) to construct the draft reference genome of sheep (Ovis aries). Component activities such as data generation, sequence assembly and annotation are described, along with information concerning the key researchers performing the work. This aims to foster future participation from across the research community through the coordinated activities of the consortium. The review also serves as a 'marker paper' by providing information concerning the pre-publication release of the reference genome. This ensures the ISGC adheres to the framework for data sharing established at the recent Toronto International Data Release Workshop and provides guidelines for data users.
Genomic blots from man, monkey, cow, sheep, pig, rabbit, dog, rat, mouse, guinea pig, and chicken DNA were hybridized with probes derived from the four exons of the human butyrylcholinesterase gene (BCHE) (Arpagaus, M., Kott, M., Vatsis, K. P., Bartels, C. F., La Du, B. N., and Lockridge, O. (1990) Biochemistry 29, 124-131). Results showed that the BCHE gene was present in a single copy in the genome of all these vertebrates. The polymerase chain reaction was used to amplify genomic DNA from these animals with oligonucleotides derived from the human BCHE coding sequence. The amplified segment contained 423 bp of BCHE sequence including the active site serine of the enzyme (amino acid 198) and a component of the anionic site, aspartate 70. Amplification was successful for monkey, pig, cow, dog, sheep, and rabbit DNA, but unsuccessful for rat, guinea pig, mouse, and chicken DNA. Amplified segments were cloned in M13 and sequenced. The mouse sequence was obtained by sequencing a genomic clone. The highest identity of the human amino acid sequence was found with monkey (100%) and the lowest with mouse (91.5%). The sequence around the active site serine 198, Phe-Gly-Glu-Ser-Ala-Gly-Ala, was conserved in all eight animals as was the anionic site component, aspartate 70. A phylogenetic tree of mammalian butyrylcholinesterases was constructed using the partial BCHE sequences.
