(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Platyhelminthes: NE > Trematoda: NE > Digenea: NE > Strigeidida: NE > Schistosomatoidea: NE > Schistosomatidae: NE > Schistosoma: NE > Schistosoma bovis: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Schistosoma curassoni: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSLGIMKNMNYYLITSFLLLNNGLSFKTNTIDNVNNIHLQSSIENTMINN NNSNLVHTDLHNDKTITCLSDNPIVHTSVGIYCGLREIVHWPNGPASMVD VYYGIRYAQSPTGSLRFKKPVEPIPEPKKIFMADKLPSTCPQPKDTMFQN SAAARMWVPNTPMSEDCLFLNIWVPLKESNSSHSNSKEKLAVMLWIYGGS FYMGTATLSVYDARFLAARQNIIVASMNYRLGSFGFLYMNTEEAPGNMGL WDQRLAMKWIKDHIENFGGDPHRITLFGESAGAVSVSTHVVSPWSHSYYN NAIMQSGSIFSNWGLATSEVSLNQTQRLAKILGCGYRSSMDQIKCLRSKS IKEILDAHDTMYDPASYFSVPFPPVLDNNFFPYENSQSFRQLKYLKPSGA LMFGINKNEGSYFLLYAFVSNSKWMKNLTDLPITNRMDYLRCLRQVLDLD DDDRPEFTEPLIRYTDFEYQTYQQLPTLESWTERLEEISSDRSFKCPTIN MATAVTNDYRIPGRRRAHTLPVYFYEFQHRTLSLPMPKWTGTMHGYEIEY VFGIPFSPQFQASFYRFTDEERQLSDIMMTYWANFARTGDPNILPDGRHV TDNVNPEDPDEITGGELEDSLNHKQGRKNPFIGWPEFRNSTKAYIVFRSA PANLLVSTRPRHRQCLFWRRWYPALLQQVERNRQHCLGV
References
Title: Expression and comparative functional characterisation of recombinant acetylcholinesterase from three species of Schistosoma Bentley GN, Jones AK, Agnew A Ref: Molecular & Biochemical Parasitology, 141:119, 2005 : PubMed
Title: Mapping and sequencing of acetylcholinesterase genes from the platyhelminth blood fluke Schistosoma Bentley GN, Jones AK, Agnew A Ref: Gene, 314:103, 2003 : PubMed
Acetylcholinesterase (AChE) on the surface of the parasitic blood fluke Schistosoma is the likely target for schistosomicidal anticholinesterases. Determination of the molecular structure of this drug target is key for the development of improved anticholinesterase drugs and potentially a novel vaccine. We have recently cloned the cDNA encoding the AChE from the human parasite Schistosoma haematobium and succeeded in expressing functional recombinant protein. We now describe the cloning and molecular characterisation of homologues from two other schistosome species-Schistosoma mansoni and Schistosoma bovis, which are important parasites of man and cattle, respectively, but which differ in their sensitivity to the therapeutic anticholinesterase metrifonate. Comparison of the deduced amino acid sequences revealed that the AChE from all three species posses a high degree of identity, with conservation of all of the residues known to be important for substrate binding and catalytic activity. Also conserved is a unique C-terminal domain which is unusual in that it lacks the consensus for GPI modification, even though the native protein is considered to be GPI-anchored. We have also established the AChE gene structures for all three species and cloned the complete gene for S. haematobium AChE. The gene structure is relatively complex, comprising nine coding exons; the location of the splice sites is identical in all three species, but the size of the introns varies considerably. The two C-terminal splicing sites that are conserved in all species are also present in Schistosoma, but a third C-terminal conserved splicing site which is located 11-13 amino acids upstream of the histidine of the catalytic triad in all invertebrate AChE genes characterised to date is absent. We discuss our findings in the context of the molecular phylogeny of the AChE genes and the potential application to the control of schistosomiasis.
