Gene_locus Report for: rhoso-a0a3g5hne7 Rhodococcus sp.Hyd-protein phthalate ester hydrolase
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Bacteria: N E > Terrabacteria group: N E > Actinobacteria [phylum]: N E > Actinobacteria [class]: N E > Corynebacteriales: N E > Nocardiaceae: N E > Rhodococcus: N E > Rhodococcus sp.: N E 6_AlphaBeta_hydrolase :
rhosr-q0rwt2 Rhodococcus sp. (strain RHA1) putative uncharacterized protein ,
rhosr-q0rwv3 Rhodococcus sp. (strain RHA1) possible 3-oxoadipate enol-lactone hydrolase ,
rhosr-q0rz78 Rhodococcus sp. (strain RHA1) probable hydrolase (EC 3.-.-.-) ,
rhosr-q0s0s0 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
rhosr-q0s1m1 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s1x5 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s2t5 Rhodococcus sp. (strain RHA1) possible esterase ,
rhosr-q0s3c8 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s4f1 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
rhosr-q0s6a9 Rhodococcus sp. (strain RHA1) hydrolase ,
rhosr-q0s6b5 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s7i7 Rhodococcus sp. (strain RHA1) hydrolase ,
rhosr-q0s7r1 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s8b3 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0s8p7 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0s9l3 Rhodococcus sp. (strain RHA1) Hydrolase ,
rhosr-q0s9m1 Rhodococcus sp. (strain RHA1) probable hydrolase. N-terminal alpha/beta-hydrolase domain and a C-terminal cyclase homology domain ,
rhosr-q0s230 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0s393 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0s849 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0saa5 Rhodococcus sp. (strain RHA1) possible alpha/beta hydrolase ,
rhosr-q0sbd3 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0sdr2 Rhodococcus sp. (strain RHA1) 3-oxoadipate enol-lactonase ,
rhosr-q0sdr5 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0ses6 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0sex8 Rhodococcus sp. (strain RHA1) probable chloride peroxidase (EC 1.11.1.10) ,
rhosr-q0sfl2 Rhodococcus sp. (strain RHA1) possible alpha/beta fold superfamily hydrolase ,
rhosr-q0shd6 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0shi2 Rhodococcus sp. (strain RHA1) Possible hydrolase ,
rhosr-q0sjy0 Rhodococcus sp. (strain RHA1) Probable hydrolase .
A85-Est-Putative :
rhosr-q0ryn3 Rhodococcus sp. (strain RHA1) Possible esterase ,
rhosr-q0s1n4 Rhodococcus sp. (strain RHA1) Possible esterase ,
rhosr-q0s6b3 Rhodococcus sp. (strain RHA1) Possible esterase ,
rhosr-q0sc04 Rhodococcus sp. (strain RHA1) Possible esterase .
A85-IroE-IroD-Fes-Yiel :
rhosr-q0s8z9 Rhodococcus sp. (strain RHA1) Enterochelin esterase .
A85-Mycolyl-transferase :
rhosr-q0s5h5 Rhodococcus sp. (strain RHA1) Possible antigenic 85 complex protein .
Abhydrolase_9 :
rhosr-q0s2i6 Rhodococcus sp. Putative uncharacterized protein ,
rhosr-q0sa61 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
9noca-a0a0b1rfg6 Rhodococcus sp.; Rhodococcus pyridinivorans Membrane protein ,
9noca-a0a0b1rh82 Rhodococcus sp.; Rhodococcus pyridinivorans, Membrane protein ,
9noca-a0a0b1rhr4 Rhodococcus sp.; Rhodococcus pyridinivorans, Uncharacterized protein ,
9noca-a0a0d0kq55 Rhodococcus sp. Contig_13, whole genome shotgun sequence ,
9noca-a0a0d5a922 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0d5acy2 Rhodococcus sp. Membrane protein ,
9noca-a0a0d5aj96 Rhodococcus sp. Membrane protein ,
9noca-a0a0k2yim8 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0k2ypj4 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0k2ypl2 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0k2yq39 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6ghw7 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6iva0 Rhodococcus sp.; Rhodococcus fascians Uncharacterized protein ,
9noca-a0a0q6ixk9 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jkb0 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jsp1 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6kl16 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6kxz1 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6lc41 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a135gp25 Rhodococcus sp. (Nocardia opaca) Rhodococcus opacus; Rhodococcus wratislaviensis; Rhodococcus imtechensis, Uncharacterized protein ,
9noca-a0a143qad7 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a143qfz0 Rhodococcus sp.; Rhodococcus fascians Uncharacterized protein ,
9noca-a0a143qnr4 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a161tf43 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a164q9g9 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a164qpb2 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a1b1gt40 Rhodococcus sp.; Rhodococcus enclensis Uncharacterized protein ,
9noca-a0a1b2ue36 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a1b2ujq4 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a1b5zbx8 Rhodococcus sp. (Corynebacterium equi); Rhodococcus equi Uncharacterized protein ,
9noca-a0a1b5zjz7 Rhodococcus sp. (Corynebacterium equi); Rhodococcus equi Uncharacterized protein ,
9noca-a0a1b5zms7 Rhodococcus sp. (Corynebacterium equi); Rhodococcus equi Uncharacterized protein ,
9noca-a0a1d8t476 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a1d8t744 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a1j0tlk3 Rhodococcus sp.; Rhodococcus pyridinivorans. Uncharacterized protein ,
9noca-a0a1j0tls6 Rhodococcus sp; Rhodococcus pyridinivorans. Uncharacterized protein ,
9noca-j2j9v7 Rhodococcus sp. Uncharacterized protein ,
9noca-l8dj70 Rhodococcus sp. Putative integral membrane protein ,
9noca-l8dme1 Rhodococcus sp. Putative integral membrane protein ,
9noca-n1m2w4 Rhodococcus sp.; Rhodococcus aetherivorans; Rhodococcus rhodochrous Predicted membrane protein (DUF2319) ,
9noca-n1m4g0 Rhodococcus sp. ; Rhodococcus aetherivorans; Rhodococcus rhodochrous, Predicted membrane protein (DUF2319) ,
9noca-n1m6r9 Rhodococcus sp. Predicted membrane protein (DUF2319) ,
9noca-n1meb1 Rhodococcus sp.; Rhodococcus aetherivorans; Rhodococcus rhodochrous, Uncharacterized protein ,
9noca-n1mgb0 Rhodococcus sp.; Rhodococcus ruber Predicted membrane protein (DUF2319) .
Abhydrolase_10 :
rhosx-a0a0d8hp07 Rhodococcus sp. (strain AD45). Uncharacterized protein .
AlphaBeta_hydrolase :
rhosr-q0s2n9 Rhodococcus sp. (strain RHA1) possible hydrolase .
Bacterial_Est97 :
rhosr-q0rz46 Rhodococcus sp. (strain RHA1) Rhodococcus imtechensis RKJ300 Putative uncharacterized protein ,
rhosr-q0sa26 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein .
Carbon-carbon_bond_hydrolase :
rhosp-EtbD2 Rhodococcus sp. DNA for EtbD2, 2-hydroxt-6-oxohepta-2,4-dienoate hydrolasecomplete cds ,
rhosp-OphC Rhodococcus sp. strain V49, 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase hydrolase ohC ,
rhosr-q0rxc8 Rhodococcus sp. (strain RHA1) Alpha/beta-fold C-C bond hydrolase ,
rhosr-q0s008 Rhodococcus sp. (strain RHA1) Alpha/beta-fold C-C bond hydrolase ,
rhosr-q0sd10 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0sdh6 Rhodococcus sp. (strain RHA1) Possible haloalkane dehalogenase ,
rhosr-q0skn1 Rhodococcus sp. (strain RHA1) probable hydrolase .
Carb_B_Bacteria :
9noca-a0a259xrg6 Rhodococcus sp., Carboxylic ester hydrolase ,
9noca-a0a259xar0 Rhodococcus sp., Carboxylic ester hydrolase ,
9noca-a0a0k2y8p3 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0k2yid9 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0k2yjx7 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jd92 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jgg6 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6ju71 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jui4 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6jz79 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6k220 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6kjx6 Rhodococcus sp. Uncharacterized protein ,
9noca-a0a0q6kyf5 Rhodococcus sp. Uncharacterized protein .
Cocaine_esterase :
rhosm-cocE .
Cutinase :
rhosr-q0sab7 Rhodococcus sp., Rhodococcus opacus, Rhodococcus imtechensis, Uncharacterized protein ,
rhosr-q0rwx4 Rhodococcus sp., Rhodococcus opacus, Uncharacterized protein ,
rhosr-q0ruu2 Rhodococcus sp. (strain RHA1) Uncharacterized protein ,
rhosr-q0s747 Rhodococcus sp. (strain RHA1) Uncharacterized protein ,
rhosr-q0ry47 Rhodococcus sp. (strain RHA1) Uncharacterized protein ,
rhosr-q0rwa1 Rhodococcus sp. (strain RHA1) Uncharacterized protein ,
rhosr-q0sj22 Rhodococcus sp. (strain RHA1) Probable cutinase .
Duf_1023 :
rhosr-q0sej4 Rhodococcus sp.; Rhodococcus opacus; Nocardia opacaPutative uncharacterized protein ,
9noca-a0a0b1rkw7 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0d5ag01 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0d8i594 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0d8i7q1 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0k2yfs0 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0k2ys39 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0q6gdr5 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0q6ivz8 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0q6k4n3 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0q6k8i8 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a0q6kcs9 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a135gl25 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a143qdx0 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a164pcb2 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a164pdb6 Rhodococcus sp., Uncharacterized protein ,
9noca-a0a164r939 Rhodococcus sp., Uncharacterized protein ,
9noca-l8dar1 Rhodococcus sp., Uncharacterized protein ,
9noca-l8dbl6 Rhodococcus sp., Uncharacterized protein ,
9noca-l8dbz9 Rhodococcus sp., Uncharacterized protein ,
9noca-l8djr1 Rhodococcus sp., Uncharacterized protein .
Epoxide_hydrolase :
rhosr-q0ryc0 Rhodococcus sp. (strain RHA1) Probable epoxide hydrolase/ haloalkane dehalogenase ,
rhosr-q0s1l0 Rhodococcus sp. (strain RHA1) possible hydrolase ,
rhosr-q0san0 Rhodococcus sp. (strain RHA1) Probable epoxide hydrolase ,
rhosr-q0scq2 Rhodococcus sp. (strain RHA1) Haloalkane dehalogenase ,
rhosr-q0sfz6 Rhodococcus sp. (strain RHA1) probable hydrolase ,
rhosr-q0sgj4 Rhodococcus sp. (strains RHA1; B4) Possible epoxide hydrolase ,
rhosr-q0sgv4 Rhodococcus sp. (strain RHA1) Probable haloalkane dehalogenase .
Fungal-Bact_LIP :
rhosr-q0s101 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
rhosr-q0s125 Rhodococcus sp., encoded on a Plasmid pRHL1 Lipase ,
rhosr-q0sdb8 Rhodococcus sp. (strain RHA1) Probable triacylglycerol lipase ,
rhosr-q0sdt1 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
rhosr-q0sfh8 Rhodococcus sp. (strain RHA1) Probable triacylglycerol lipase .
Haloperoxidase :
rhosp-cpo Rhodococcus sp. S9 chloroperoxidase gene ,
rhosr-q0sf05 Rhodococcus sp. (strain RHA1) Rhodococcus opacus (strain B4) probable chloride peroxidase (EC 1.11.1.10) ,
rhosr-q0sgw4 Rhodococcus sp. (strain RHA1) Rhodococcus opacus (strain B4) chloride peroxidase (EC 1.11.1.10) .
HNLyase_Bact :
rhosr-q0s837 Rhodococcus sp. (strain RHA1) Possible esterase .
Hormone-sensitive_lipase_like :
rhosp-hercx ,
rhosr-q0s1x6 Rhodococcus sp. (strain RHA1) possible esterase ,
rhosr-q0sdu5 Rhodococcus sp. (strain RHA1) probable sterase / lipase .
Monoglyceridelipase_lysophospholip :
rhosr-q0s3s6 Rhodococcus sp. (strain RHA1) Possible lysophospholipase ,
rhosr-q0s252 Rhodococcus sp. (strain RHA1) Rhodococcus opacus (strain B4) Putative uncharacterized protein .
PE-PPE :
rhosr-q0s5m7 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein ,
rhosr-q0s477 Rhodococcus sp. (strain RHA1) Putative uncharacterized protein .
PHA_synth_III_C :
rhosr-q0s8f4 Rhodococcus sp. (strain RHA1) Probable poly-beta-hydroxybutyrate polymerase .
Proline_iminopeptidase :
rhosr-q0saw3 Rhodococcus sp. (strain RHA1) Proline iminopeptidase .
UCP037442 :
rhosr-q0set7 Rhodococcus sp. (strain RHA1)Rhodococcus opacus (strain B4) putative uncharacterized protein Molecular evidence
Database
Sequence
Graphical view for this peptide sequence: rhoso-a0a3g5hne7 Colored MSA for 6_AlphaBeta_hydrolase (raw)
MVGVSATDLLHTHLFGPVDGPEVLALHGLTGHGRRWRNLGERHLPHLRFV
APDLRGHGRSPWTPPWSFEAHIADLKAVLDAHTTGPVTVVGHSFGGALAL
HLAAAVPDRVRALVLLDPAIGLPADRMLEIADLTLHNPDYTDEAEARAEK
VHGDWGEVAPELLDEEIAEHLVELDSGRVNWRICVPALVTSWGELARGPV
LPPAGIPTIFVQAGKVQPPYTTSNFRRDLAERLGDDLTLLEFDCDHMIDQ
ARPAETAELVARMV
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M V G V S A T D L L H T H L F G P V D G P E V L A L H G L T G H G R R W R N L G E R H L P H L R F V A P D L R G H G R S P W T P P W S F E A H I A D L K A V L D A H T T G P V T V V G H S F G G A L A L H L A A A V P D R V R A L V L L D P A I G L P A D R M L E I A D L T L H N P D Y T D E A E A R A E K V H G D W G E V A P E L L D E E I A E H L V E L D S G R V N W R I C V P A L V T S W G E L A R G P V L P P A G I P T I F V Q A G K V Q P P Y T T S N F R R D L A E R L G D D L T L L E F D C D H M I D Q A R P A E T A E L V A R M V
References
Title: Mechanistic insight into esterase-catalyzed hydrolysis of phthalate esters (PAEs) based on integrated multi-spectroscopic analyses and docking simulationDu H , Hu RW , Zhao HM , Huang HB , Xiang L , Liu BL , Feng NX , Li H , Li YW and Mo CH <1 more author(s)> Du H , Hu RW , Zhao HM , Huang HB , Xiang L , Liu BL , Feng NX , Li H , Li YW , Cai QY , Mo CH (- 1) Ref: J Hazard Mater, 408 :124901, 2020 : PubMed Abstract ESTHER: Du_2020_J.Hazard.Mater_408_124901 PubMedSearch: Du 2020 J.Hazard.Mater 408 124901 PubMedID: 33360702 Gene_locus related to this paper: rhoso-a0a3g5hne7 Abstract
A novel PAE-hydrolyzing esterase (named Hyd) gene was screened from the genomic library of Rhodococcus sp. 2G and was successfully expressed in heterologous E. coli, which was defined as a new family of esterolytic enzymes. The purified Hyd could efficiently degrade various PAEs, displaying high activity and stability with a broad range of pH (4-10) and temperature (20-60 degreesC). Interaction mechanism of Hyd with dibutyl phthalate (DBP) was investigated by integrated multi-spectroscopic and docking simulation methods. Fluorescence and UV-vis spectra revealed that DBP could quench the fluorescence of Hyd through a static quenching mechanism. The results from synchronous fluorescence and CD spectra confirmed that the DBP binding to Hyd triggered conformational and micro-environmental changes of Hyd, which were characterized by increased stretching extent and random coil, and decreased alpha-helix and beta-sheet. Molecular docking study showed that DBP could be bound to the cavity of Hyd with hydrogen bonding and hydrophobic interaction. A novel and distinctive catalytic mechanism was proposed: two key residues Thr(190) and Ser(191) might catalyze the hydrolysis of DBP, instead of the conserved catalytic triad (Ser-His-Asp) reported elsewhere, which was confirmed by site-directed mutagenesis.
Title: Bioaugmentation of Exogenous Strain Rhodococcus sp. 2G Can Efficiently Mitigate Di(2-ethylhexyl) Phthalate Contamination to Vegetable CultivationZhao HM , Du H , Huang CQ , Li S , Zeng XH , Huang XJ , Xiang L , Li H , Li YW and He Z <2 more author(s)> Zhao HM , Du H , Huang CQ , Li S , Zeng XH , Huang XJ , Xiang L , Li H , Li YW , Cai QY , Mo CH , He Z (- 2) Ref: Journal of Agricultural and Food Chemistry, 67 :6940, 2019 : PubMed Abstract ESTHER: Zhao_2019_J.Agric.Food.Chem_67_6940 PubMedSearch: Zhao 2019 J.Agric.Food.Chem 67 6940 PubMedID: 31021627 Gene_locus related to this paper: rhoso-a0a3g5hne7 Abstract
This work developed a bioaugmentation strategy that simultaneously reduced soil di(2-ethylhexyl) phthalate (DEHP) pollution and its bioaccumulation in Brassica parachinensis by inoculating the isolated strain Rhodococcus sp. 2G. This strain could efficiently degrade DEHP at a wide concentration range from 50 to 1600 mg/L and transformed DEHP through a unique biochemical degradation pathway that distinguished it from other Rhodococcus species. Besides, strain 2G colonized well in the rhizosphere soil of the inoculated vegetable without competition with indigenous microbes, resulting in increased removal of DEHP from soil (95%) and reduced DEHP bioaccumulation in vegetables (75% in the edible part) synchronously. Improved enzyme activities and DOC content in the rhizosphere of the planting vegetable and inoculating strain 2G were responsible for the high efficiency in mitigating DEHP contamination to vegetable cultivation. This work demonstrated a great potential application to grow vegetables in contaminated soil for safe food production.
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