Gene_locus Report for: ratno-q5ylm1 Rattus norvegicus (Rat) neural stem cell-derived dendrite regulator Diacylglycerol lipase-alpha Dagla
Comment
only central part Pfam A Lipase_3 394 534 Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Deuterostomia: N E > Chordata: N E > Craniata: N E > Vertebrata: N E > Gnathostomata: N E > Teleostomi: N E > Euteleostomi: N E > Sarcopterygii: N E > Dipnotetrapodomorpha: N E > Tetrapoda: N E > Amniota: N E > Mammalia: N E > Theria: N E > Eutheria: N E > Boreoeutheria: N E > Euarchontoglires: N E > Glires: N E > Rodentia: N E > Myomorpha: N E > Muroidea: N E > Muridae: N E > Murinae: N E > Rattus: N E > Rattus norvegicus: N E A85-EsteraseD-FGH :
rat-estd Rattus norvegicus (Rat) Esterase D .
ABHD6-Lip :
rat-abhd6 Rattus norvegicus (Rat) Abhydrolase domain-containing protein 6 .
ABHD8 :
rat-b5den3 Rattus norvegicus (Rat). Abhydrolase domain-containing 8 .
ABHD10 :
rat-abhda Rattus norvegicus (Rat) Abhydrolase domain-containing protein 10, mitochondrial .
ABHD11-Acetyl_transferase :
rat-d3zxk4 Rattus norvegicus (Rat). Abhydrolase domain-containing 11 .
ABHD12-PHARC :
ratno-q6ayt7 Rattus norvegicus (Rat) ABD12 Lysophosphatidylserine lipase ABHD12 .
ABHD13-BEM46 :
rat-d4a1b6 Rattus norvegicus (Rat). Similar to 1110065L07Rik protein (Predicted), isoform CRA_b .
ABHD16 :
ratno-q5xil6 Rattus norvegicus (Rat) rgd1309726_predicted protein ,
ratno-q6mg55 Rattus norvegicus (Rat) hla-b associated transcript 5, rat orthologue .
ABHD17-depalmitoylase :
ratno-q5xij5 Rattus norvegicus (Rat) similar to cdna sequence bc005632 AB17A ,
ratno-q6ay17 Rattus norvegicus (Rat) AB17B hypothetical protein ,
rat-ab17c Rattus norvegicus (Rat). Alpha/beta hydrolase domain-containing protein 17C .
ABHD18 :
rat-cd029 Rattus norvegicus (Rat) Uncharacterized protein ABHD18 C4orf29 homolog .
abh_upf0017 :
rat-d4a7w1 Rattus norvegicus (Rat) Protein Abhd2 ,
ratno-ABH15 Rattus norvegicus (Rat) Abhydrolase domain-containing 15 ,
ratno-abhd1 Rattus norvegicus (Rat) abhydrolase domain containing 1 (predicted) ,
ratno-d4a3d4 Rattus norvegicus (Rat) lung alpha/beta hydrolase fold protein 3 .
ACHE :
ratno-ACHE Rattus norvegicus (Rat) Acetylcholinesterase .
Acidic_Lipase :
rat-d3zuq1 Rattus norvegicus (Rat) Lipase ,
rat-d4a9l7 Rattus norvegicus (Rat) Lipase ,
rat-d4aa33 Rattus norvegicus (Rat) Lipase ,
rat-d4aa61 Rattus norvegicus (Rat) Lipase ,
ratno-1lipg Rattus norvegicus (Rat) Lingual lipase ,
ratno-1llip Rattus norvegicus (Rat) lysosomal acid lipase, intracellular hydrolase rats, Wolman, liver mRNA .
ACPH_Peptidase_S9 :
ratno-acph Rattus norvegicus (Rat) Acyl-peptide hydrolase; Acylamino-acid-releasing enzyme .
Acyl-CoA_Thioesterase :
ratno-acot1 Rattus norvegicus (Rat) hydrolase) (cte-I) (lach2) (ach2) ,
ratno-acot2 Rattus norvegicus (Rat) (EC 3.1.2.2) ACOT2 mitochondrial Acyl-CoA thioesterase 1 very-long-chain Acyl-CoA thioesterase (MTE-I) ,
ratno-BAAT Rattus norvegicus (Rat) bile acid CoA: amino acid n-acyltransferase kan-1 (EC 3.1.2.2) ,
ratno-q5fvr5 Rattus norvegicus (Rat) hypothetical loc313220 .
Arylacetamide_deacetylase :
rat-nceh1 Rattus norvegicus (Rat) Arylacetamide deacetylase-like 1 ,
ratno-aryla Rattus norvegicus (Rat) Arylacetamide deacetylase gene (aadac) ,
rat-d3zba8 Rattus norvegicus (Rat). Similar to arylacetamide deacetylase (Predicted) ,
rat-d3zbj1 Rattus norvegicus (Rat). Similar to novel protein similar to esterases ,
rat-d3zcr8 Rattus norvegicus (Rat). Arylacetamide deacetylase-like 3 ,
rat-d3zxw5 Rattus norvegicus (Rat). Similar to hypothetical protein C130079G13 ,
rat-d4a340 Rattus norvegicus (Rat). Similar to arylacetamide deacetylase ,
rat-f1lvg7 Rattus norvegicus (Rat). Similar to hypothetical protein C130079G13 ,
rat-m0r509 Rattus norvegicus (Rat). Similar to hypothetical protein C130079G13 ,
rat-m0r5d4 Rattus norvegicus (Rat). Arylacetamide deacetylase-like 2 .
BCHE :
ratno-BCHE Rattus norvegicus (Rat) Butyrylcholinesterase .
Carboxypeptidase_S10 :
ratno-CPVL Rattus norvegicus (Rat) hypothetical protein loc502774 ,
ratno-Ppgb Rattus norvegicus (Rat) Lysosomal protective protein, Cathepsin A Ctsa, Protective protein for beta-galactosidase Ppgb ,
ratno-RISC Rattus norvegicus (Rat) retinoid-inducible serine carboxypeptidase precursor .
Carb_B_Chordata :
ratno-cauxin Rattus norvegicus (Rat) Carboxylesterase 5A (old est7) Epididymis-specific carboxylesterase 615 protein Carboxylesterase-like urinary excreted protein homolog ,
ratno-Ces1c Rattus norvegicus (Rat) Neutral retinyl ester hydrolase ,
ratno-Ces1d Rattus norvegicus (Rat) Carboxylesterase (ES-HVEL) pI 6.1 esterase (ES-10) CES1D Ces1d ,
ratno-Ces1e Rattus norvegicus (Rat) Liver carboxylesterase Carboxylesterase 1E ES-3 (egasyn) ,
ratno-Ces2f Rattus norvegicus (Rat) Protein Ces2f ,
ratno-CESRRL1 Rattus norvegicus (Rat) carboxylesterase rl1 ,
ratno-d3ze31 Rattus norvegicus (Rat) Putative uncharacterized protein RGD1565045 ,
ratno-d3zp14 Rattus norvegicus (Rat) Putative uncharacterized protein LOC685645 ,
ratno-d3zxq0 Rattus norvegicus (Rat) Similar to 2210023G05Rik protein (Predicted) ,
ratno-d3zxq1 Rattus norvegicus (Rat) Putative uncharacterized protein ENSRNOP00000044470 ,
ratno-d4aa05 Rattus norvegicus (Rat) Putative uncharacterized protein LOC679817 ,
ratno-est8 Rattus norvegicus (Rat) RCG51618 ,
ratno-kmcxe Rattus norvegicus (Rat) Liver carboxylesterase 4, kydney microsomal carboxylesterase Liver carboxylesterase 4 ES-4 ,
ratno-lmcxe Rattus norvegicus (Rat) Liver microsomal carboxylesterase Ces1f ,
ratno-LOC246252 Rattus norvegicus carboxylesterase isoenzyme gene (LOC246252) Ces1h EST2A Ces2a ,
ratno-pbcxe Rattus norvegicus (Rat) Phenobarbital-inducible carboxylesterase Ces2c ,
ratno-phebest Rattus norvegicus (Rat) Carboxylesterase, partial cds ,
ratno-q4qr68 Rattus norvegicus (Rat) hypothetical protein Ces2b ,
ratno-q32q55 Rattus norvegicus (Rat) hypothetical protein ,
ratno-q68g49 Rattus norvegicus (Rat) loc291863 protein ,
ratno-sicxe Rattus norvegicus (Rat) small intestinal carboxylesterase precursor ,
rat-a0a0g2k9y7 Rattus norvegicus (Rat). Carboxylic ester hydrolase ,
rat-a0a0g2kb83 Rattus norvegicus (Rat). Carboxylic ester hydrolase .
CGI-58_ABHD5_ABHD4 :
rat-d3zaw4 Rattus norvegicus (Rat) Uncharacterized protein ,
ratno-abhd5 Rattus norvegicus (Rat) cgi-58-like protein .
Cholesterol_esterase :
ratno-balip Rattus norvegicus (Rat) Cholesterol esterase .
CIB-CCG1-interacting-factor-B :
rat-abhea Rattus norvegicus (Rat) Abhydrolase domain-containing protein 14A ,
rat-abheb Rattus norvegicus (Rat) Abhydrolase domain-containing protein 14B .
CMBL :
ratno-CMBL Rattus norvegicus (Rat) ab2-225 (2310016a09rik protein) .
DPP4N_Peptidase_S9 :
rat-dpp9 ,
rat-d3zhq1 Rattus norvegicus (Rat) DPP8 ,
ratno-dpp4 ,
ratno-dpp6 Rattus norvegicus (Rat) Dipeptidyl aminopeptidase-like protein 6 (dipeptidylpeptidase VI) (dppx) ,
ratno-FAP Rattus norvegicus (Rat) fibroblast activation protein alpha subunit ,
ratno-q6q629 Rattus norvegicus (Rat) DPP-10 Dipeptidyl peptidase IV-related , kv4 potassium channel auxiliary subunit .
Duf_676 :
rat-d3zxw8 Rattus norvegicus (Rat) Protein Fam135a Similar to KIAA1411 protein ,
rat-f1lz91 Rattus norvegicus (Rat) Protein RGD1308133 .
Duf_726 :
rat-tmco4 Rattus norvegicus (Rat) Transmembrane and coiled-coil domain-containing protein 4 .
Epoxide_hydrolase :
rat-d3zkp8 Rattus norvegicus (Rat) Uncharacterized protein ,
rat-d4a4w4 Rattus norvegicus (Rat) Uncharacterized protein ,
ratno-hyep Rattus norvegicus (Rat) Epoxide hydrolase 1, Epoxide hydratase ,
ratno-hyes Rattus norvegicus (Rat) Bifunctional epoxide hydrolase 2, cytosolic epoxide hydrolase, Lipid-phosphate phosphatase .
Hepatic_Lipase :
ratno-1hlip Rattus norvegicus (Rat) Triacylglycerol lipase hepatic .
Hormone-sensitive_lipase_like :
ratno-hslip Rattus norvegicus (Rat) Hormone sensitive lipase .
Hydrolase_RBBP9_YdeN :
ratno-rbbp9 Rattus norvegicus (Rat) Retinoblastoma-binding protein 9 B5T overexpressed gene protein (bog protein) .
Kynurenine-formamidase :
rat-m0rc77 Rattus norvegicus (Rat). N-formylkynurenine formamidase .
LIDHydrolase :
rat-Ldah Rattus norvegicus (Rat) UPF0554 protein LDAH C2orf43 homolog .
Lipase_3 :
rat-dglb Rattus norvegicus (Rat) Sn1-specific diacylglycerol lipase beta .
Lipoprotein_Lipase :
ratno-lipli Rattus norvegicus (Rat) Lipoprotein lipase ,
ratno-q5d216 Rattus norvegicus (Rat) endothelial lipase Lipg .
LYsophospholipase_carboxylesterase :
ratno-lypla1a Rattus norvegicus (Rat) Lysophospholipase (Pla1a) ,
ratno-lypla2 Rattus norvegicus (Rat) Lysophospholipase II .
Maspardin-ACP33-SPG21_like :
ratno-SPG21 Rattus norvegicus (Rat) acid cluster protein 33 (Spg21) Maspardin .
MEST-like :
ratno-q6p5p5 Rattus norvegicus (Rat) Mesoderm-specific transcript homolog protein .
Monoglyceridelipase_lysophospholip :
ratno-MGLL Rattus norvegicus (rat) monoglyceride lipase (Mgl2) .
Ndr_family :
ratno-ndr4 Rattus norvegicus (Rat) ndrg4 protein (brain development-related molecule 1) ndrg4-b1 ndrg4-a2 ,
ratno-NDRG2 Rattus norvegicus (Rat) ndrg1 related protein ndrg2b1 (ndrg1 related protein ndrg2a1) ,
ratno-q6ayr2 Rattus norvegicus (Rat) hypothetical protein ,
ratno-q6je36 Rattus norvegicus (Rat) n-myc downstream regulated 1 (hypothetical protein) .
Neuroligin :
ratno-1neur ,
ratno-2neur ,
ratno-3neur Rattus norvegicus (Rat) Neuroligin 3 Nlgn3 .
NLS3-Tex30 :
rat-Kansl3 Rattus norvegicus (Rat) Non-specific lethal 3 homologue Uncharacterized protein KIAA1310 homolog ,
rat-Tex30 Rattus norvegicus (Rat) Putative uncharacterized protein RGD1309095_predicted .
PAF-Acetylhydrolase :
ratno-paf2 Rattus norvegicus (Rat) PAFAHII platelet-activating factor acetylhydrolase II ,
ratno-q5m7t7 Rattus norvegicus (Rat) acetylhydrolase, plasma) (predicted) .
Palmitoyl-protein_thioesterase :
ratno-ppt Rattus norvegicus (Rat) Palmitoyl-protein thioesterase 1 ,
ratno-PPT2 Rattus norvegicus (Rat) palmitoyl-protein thioesterase 2, EC 3.1.2.22 .
Pancreatic_lipase :
ratno-1plip Rattus norvegicus (Rat) Pancreatic lipase ,
ratno-3plip Rattus norvegicus (Rat) Pancreatic lipase-related protein 1 precursor (EC 3.1.1.3) ,
ratno-4plip .
PC-sterol_acyltransferase :
ratno-lcat Rattus norvegicus (Rat) Phosphatidylcholine-sterol acyltransfer ,
ratno-q675a5 Rattus norvegicus (Rat) lysosomal phospholipase a2 .
Pectinacetylesterase-Notum :
ratno-d3zxi3 Rattus norvegicus (Rat) Notum .
PGAP1 :
ratno-q765a7 Rattus norvegicus (Rat) gpi deacylase .
Phospholipase :
ratno-P97535 Rattus norvegicus (Rat) ps-pla1 precursor ,
ratno-q6p6s8 Rattus norvegicus (Rat) hypothetical protein (fragment) ,
rat-d3zmg4 Rattus norvegicus (Rat). Lipase, member H (Predicted) .
Prolylcarboxypeptidase :
rat-d4aa31 Rattus norvegicus (Rat) Protein Prcp ,
ratno-dpp2 Rattus norvegicus (Rat) Dipeptidyl aminopeptidase II (quiescent cell proline dipeptidase) ,
ratno-q3mhs0 Rattus norvegicus (Rat) protease, serine, 16 (thymus) .
S9N_PPCE_Peptidase_S9 :
ratno-RPOP Rattus norvegicus (Rat) Prolyl endopeptidase rPop .
S9N_PREPL_Peptidase_S9 :
ratno-q5hza6 Rattus norvegicus (Rat) similar to riken cdna d030028o16 (predicted) .
SERHL :
rat-d4a071 Rattus norvegicus (Rat) Similar to serine hydolase like protein, isoforms Serhl-2 (Predicted) .
Thioesterase :
ratno-fas Rattus norvegicus (Rat) fatty acid synthase FAS Thioesterase domain ,
ratno-sast Rattus norvegicus (Rat) (thioesterase II) .
Thyroglobulin :
ratno-thyro Rattus norvegicus (Rat) Thyroglobulin .
Valacyclovir-hydrolase :
ratno-q3b8n9 Rattus norvegicus (Rat) biphenyl hydrolase-like , breast epithelial mucin-assoc AG BPHL Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: ratno-q5ylm1 Colored MSA for Lipase_3 (raw)
MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEA
CSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYV
RLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVC
ITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKV
FLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAK
RNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFAL
AAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCN
AIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGT
LSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLS
QAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPP
GGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTK
PKWRIIVGATKCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPL
YPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHE
HLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEV
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M P G I V V F R R R W S V G S D D L V L P A I F L F L L H T T W F V I L S V V L F G L V Y N P H E A C S L N L V D H G R G Y L G I L L S C M I A E M A I I W L S M R G G I L Y T E P R D S M Q Y V L Y V R L A I L V I E F I Y A I V G I V W L T Q Y Y T S C N D L T A K N V T L G M V V C N W V V I L S V C I T V L C V F D P T G R T F V K L R A T K R R Q R N L R T Y N L R H R L E E G Q A T S W S R R L K V F L C C T R T K D S Q S D A Y S E I A Y L F A E F F R D L D I V P S D I I A G L V L L R Q R Q R A K R N A V L D E A N N D I L A F L S G M P V T R N T K Y L D L K N S H E M L R Y K E V C Y Y M L F A L A A Y G W P M Y L M R K P T C G L C Q L A R S C S C C L C P A R P R F A P G V T I E E D N C C G C N A I A I R R H F L D E N M T A V D I V Y T S C H D A V Y E T P F Y V A V D H D K K K V V I S I R G T L S P K D A L T D L T G D A E R L P V E G H R G T W L G H K G M V L S A E Y I K K K L E Q E M V L S Q A F G R D L G R G T K H Y G L I V V G H S L G A G T A A I L S F L L R P Q Y P T L K C F A Y S P P G G L L S E D A M E Y S K E F V T A V V L G K D L V P R I G L S Q L E G F R R Q L L D V L Q R S T K P K W R I I V G A T K C I P K S E L P E D Q V E V T A L A S T R L W T H P S D L T I A L S A S T P L Y P P G R I I H V V H N H P A E Q C C C C E Q E E P T Y F A I W G D N K A F N E V I I S P A M L H E H L P Y V V M E G L N K V L E N Y N K G K T A L L S A A K V M V S P T E V
Reference
Title: Endocannabinoid 2-Arachidonoylglycerol Synthesis and Metabolism at Neuronal Nuclear Matrix Fractions Derived from Adult Rat Brain CortexAretxabala X , Garcia Del Cano G , Barrondo S , Lopez De Jesus M , Gonzalez-Burguera I , Saumell-Esnaola M , Goicolea MA , Salles J Ref: Int J Mol Sci, 24 :, 2023 : PubMed Abstract ESTHER: Aretxabala_2023_Int.J.Mol.Sci_24_3165 PubMedSearch: Aretxabala 2023 Int.J.Mol.Sci 24 3165 PubMedID: 36834575 Gene_locus related to this paper: human-ABHD12 ,
human-DAGLA ,
ratno-q5ylm1 ,
ratno-q6ayt7 Abstract
In this report, we describe the kinetics characteristics of the diacylglycerol lipase-alpha (DGLalpha) located at the nuclear matrix of nuclei derived from adult cortical neurons. Thus, using high-resolution fluorescence microscopy, classical biochemical subcellular fractionation, and Western blot techniques, we demonstrate that the DGLalpha enzyme is located in the matrix of neuronal nuclei. Furthermore, by quantifying the 2-arachidonoylglycerol (2-AG) level by liquid chromatography and mass spectrometry when 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG) was exogenously added as substrate, we describe the presence of a mechanism for 2-AG production through DGLalpha dependent biosynthesis with an apparent K(m) (K(m)(app)) of 180 microM and a V(max) of 1.3 pmol min(-1) microg(-1) protein. We also examined the presence of enzymes with hydrolytic and oxygenase activities that are able to use 2-AG as substrate, and described the localization and compartmentalization of the major 2-AG degradation enzymes, namely monoacylglycerol lipase (MGL), fatty acid amide hydrolase (FAAH), alpha/beta-hydrolase domain 12 protein (ABHD12) and cyclooxygenase-2 (COX2). Of these, only ABHD12 exhibited the same distribution with respect to chromatin, lamin B1, SC-35 and NeuN as that described for DGLalpha. When 2-AG was exogenously added, we observed the production of arachidonic acid (AA), which was prevented by inhibitors (but not specific MGL or ABHD6 inhibitors) of the ABHD family. Overall, our results expand knowledge about the subcellular distribution of neuronal DGLalpha, and provide biochemical and morphological evidence to ensure that 2-AG is produced in the neuronal nuclear matrix. Thus, this work paves the way for proposing a working hypothesis about the role of 2-AG produced in neuronal nuclei.
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