(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Pezizomycotina: NE > Pezizomycetes: NE > Pezizales: NE > Pyronemataceae: NE > Pyronema: NE > Pyronema omphalodes: NE
Fungal_carboxylesterase_lipase : pyrom-u4ltg1Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Carboxylesterase 4A acc. no. Q8R0W5, pyrom-u4lhe1Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to esterase acc. no. O62760, pyrom-u4kwa3Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Lipase 3 acc. no. P32947, pyrom-u4lfu9Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Lipase 4 acc. no. P32948, pyrom-u4l0a5Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Lipase 3 acc. no. Q96VC9, pyrom-u4l0n5Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Lipase 1 acc. no. P79066, pyrom-u4lhv4Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to esterase acc. no. P04058, pyrom-u4l8w9Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Carboxylesterase acc. no. P86325, pyrom-u4l9c9Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to Lipase 3 acc. no. Q96VC9, pyrom-u4lwe6Pyronema omphalodes (strain CBS 100304) (Pyronema confluens) Similar to esterase acc. no. Q92035. Hormone-sensitive_lipase_like : pyrom-u4l3n9Pyronema omphalodes (strain CBS 100304) (Pyronema confluens). Similar to Putative alpha/beta hydrolase R526 acc. no. Q5UQ83
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MATPEVFSDLLQTTFKGVQTADGVFQFRNIKYASVPKRFARSELVKSYEG QVVDATEHGPHPPGRIVPPGGDAEIALFAIPEAKRYPVQPIHDEFDCLNL SITIPATQTELLPVLINIPGGANTRCINGISLFDYTRLVSDSIAQKKPII AIHVPYRVGLFGYGLLPNGKGGGNNGLFDQRNAFEWVKRHIKAFGGDPAN ITVAGESAGAFSVDCHLQSNDSVPYFRRAVLQSGTLRVLRPSSVEKRVEL TRRAAAALGFDGEDWPERLAAASVEDIRQAQDKIGMFSISAVDDGEFFQK GIDVGKPIVPEWVDSIMIGDCGYEGFLWLPKFKHIEASKIIAVLESQGEL GKAVLTAYPGLTSSDREVSLDATLYLLQDLSFSHPNSALTAAYKALDKPV YSYLFDQHNPWEPKKHRSHHACDLLFWFNGYDFSSTVDAKGNQDVSEAMK RALISYTNGENPWDDSEVMGFGPDGFVGTVAKENEGKTRRVHVYEILDKY PAEEVLKLSDVILAV
Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding of fungal evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage of filamentous ascomycetes, there is so far only one genome available, namely that of the black truffle, Tuber melanosporum, a mycorrhizal species with unusual subterranean fruiting bodies. To help close the sequence gap among basal filamentous ascomycetes, and to allow conclusions about the evolution of fungal development, we sequenced the genome and assayed transcriptomes during development of Pyronema confluens, a saprobic Pezizomycete with a typical apothecium as fruiting body. With a size of 50 Mb and ~13,400 protein-coding genes, the genome is more characteristic of higher filamentous ascomycetes than the large, repeat-rich truffle genome; however, some typical features are different in the P. confluens lineage, e.g. the genomic environment of the mating type genes that is conserved in higher filamentous ascomycetes, but only partly conserved in P. confluens. On the other hand, P. confluens has a full complement of fungal photoreceptors, and expression studies indicate that light perception might be similar to distantly related ascomycetes and, thus, represent a basic feature of filamentous ascomycetes. Analysis of spliced RNA-seq sequence reads allowed the detection of natural antisense transcripts for 281 genes. The P. confluens genome contains an unusually high number of predicted orphan genes, many of which are upregulated during sexual development, consistent with the idea of rapid evolution of sex-associated genes. Comparative transcriptomics identified the transcription factor gene pro44 that is upregulated during development in P. confluens and the Sordariomycete Sordaria macrospora. The P. confluens pro44 gene (PCON_06721) was used to complement the S. macrospora pro44 deletion mutant, showing functional conservation of this developmental regulator.
