Gene_locus Report for: psesp-PURase

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Gammaproteobacteria: N E > Pseudomonadales: N E > Pseudomonadaceae: N E > Pseudomonas: N E > Pseudomonas sp.: N E

6_AlphaBeta_hydrolase : psesp-est5Pseudomonas sp. est5 gene for esterase V, complete cds.
ABHD6-Lip : pseuo-l1hns5Pseudomonas sp. (strain M1) Putative lipase.
Abhydrolase_9 : 9psed-a0a0t6vnn4Pseudomonas sp.; Pseudomonas stutzeri, Uncharacterized protein.
abh_upf00227 : pseuo-w5irb8Pseudomonas sp. (strain M1). Esterase.
AlphaBeta_hydrolase : psesp-OPH Pseudomonas sp. pvaB. oxidized polyvinyl alcohol hydrolase precursor, sphs1-OPH Sphingopyxis sp. (strain 113P3) Oxidized polyvinyl alcohol hydrolase.
Bacterial_lip_FamI.1 : psesp-llipaPseudomonas species (strain 109) gene for lactonizing lipase.
Bacterial_lip_FamI.3 : psesp-Q52515Pseudomonas sp lipase.
Bacterial_lip_FamI.8 : 9psed-a0a2t5zmw9Pseudomonas sp. Esterase/lipase, 9psed-a0a329j3w4Pseudomonas sp. Alpha/beta hydrolase.
BD-FAE : 9psed-a0a031fw21Pseudomonas sp. Putative hydrolase, 9psed-a0a0c2rd33Pseudomonas sp. Uncharacterized protein, 9psed-a0a0j8g513Pseudomonas sp. Uncharacterized protein, 9psed-a0a0n1db25Pseudomonas sp. Uncharacterized protein, 9psed-a0a0p7kct2Pseudomonas sp. Uncharacterized protein.
CarbLipBact_2 : 9psed-j2nei8Pseudomonas sp.; Pseudomonas chlororaphis Esterase/lipase.
Carbon-carbon_bond_hydrolase : psesl-bphDPseudomonas sp. KKS102 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD), psesp-bphdPseudomonas sp. SY5 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase bphD gene, psesp-pcbDPseudomonas sp.DJ-12 PcbD mhpC 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase.
Carboxymethylbutenolide_lactonase : psesp-CATDPseudomonas sp.; Pseudomonas knackmussii 3-oxoadipate enol-lactone hydrolase, 9psed-w6w1j7Pseudomonas sp. 3-oxoadipate enol-lactonase.
Est-OsmC : 9psed-a0a1e5x9r8Pseudomonas sp. esterase found in n-term of an OsmC/Ohr family domain.
Hormone-sensitive_lipase_like : psesp-MT761613Pseudomonas sp. strain E2-15 esterase Est19 (est19) gene, 9psed-s6hcr7Pseudomonas sp.; Pseudomonas syringae Lipolytic protein, 9psed-w2dxv9Pseudomonas sp.; Pseudomonas azotoformans Lipolytic protein, 9psed-a0a1c1wn01Pseudomonas sp. Lipase, 9psed-a0a1c3gk52Pseudomonas sp.; Pseudomonas marginalis Carboxylesterase NlhH, 9psed-a0a1b5eus5Pseudomonas sp. Carboxylesterase NlhH, 9psed-a0a1e4xnj2Pseudomonas sp.; Pseudomonas fluorescens Lipase, 9psed-a0a1c1vx56Pseudomonas sp. Lipase, 9psed-a0a1x7ebc1Pseudomonas sp. Acetyl esterase, 9psed-a0a0q7akq2Pseudomonas sp. Lipase, 9psed-a0a0p7jkp4Pseudomonas sp. Lipase, 9psed-a0a031irz8Pseudomonas sp. Lipolytic protein, 9psed-a0a1b5cnf5Pseudomonas sp. Carboxylesterase NlhH, 9psed-m4x395Pseudomonas sp. Putative lipolytic enzyme, 9psed-a0a1r0g376Pseudomonas sp. Lipase, 9psed-a0a0m9att2Pseudomonas sp. Lipase, 9psed-j1ibj5Pseudomonas sp; Pseudomonas fluorescens. Lipolytic protein, 9psed-a0a1n7b956Pseudomonas sp. Acetyl esterase, 9psed-a0a1b5d839Pseudomonas sp. Carboxylesterase NlhH, 9psed-a0a1v9uly5Pseudomonas sp. Lipase, 9psed-a0a1k2b7i4Pseudomonas sp. Acetyl esterase, 9psed-a0a1x7nvt7Pseudomonas sp.; Pseudomonas chlororaphis Acetyl esterase, 9psed-a0a0j8g0f8Pseudomonas sp. Lipase, 9psed-a0a1v3s6s7Pseudomonas sp. Lipase, 9psed-j3gq95Pseudomonas sp. Esterase/lipase, 9psed-a0a0d6bju3Pseudomonas sp. Triacylglycerol lipase, 9psed-a0a0c2m922Pseudomonas sp. Lipase, 9psed-j3gvt6Pseudomonas sp. Esterase/lipase, 9psed-a0a173jbl4Pseudomonas sp. Lipolytic protein, 9psed-a0a1b4x5q9Pseudomonas sp. Lipolytic protein ; EC=3.1.1.-, 9psed-a0a1k1ril5Pseudomonas sp. Acetyl esterase.
NLS3-Tex30 : psedt-f0e5r6Pseudomonas sp. (strain TJI-51) Uncharacterized protein.
PHA_depolymerase_arom : psesp-Est33Pseudomonas sp. strain E5-12 Esterase Est33.
S9N_PREPL_Peptidase_S9 : psesp-DAPPseudomonas sp dipeptidyl aminopeptidase.
T6SS-TLE3 : 9psed-j2qkc5Pseudomonas sp., DUF3274 domain-containing protein, 9psed-a0a191yqf1Pseudomonas sp., DUF3274 domain-containing protein, 9psed-j3gyl1Pseudomonas sp., DUF3274 domain-containing protein.
VirJ : 9psed-a0a0f0fdp7Pseudomonas sp. 2(2015). Virulence factor family protein

Title: Cloning, expression and characterization of PURase gene from Pseudomonas sp. AKS31
Saha M, Dutta SP, Mukherjee G, Basu A, Majumder D, Sil AK
Ref: Arch Microbiol, 204:498, 2022 : PubMed
Abstract


ESTHER: Saha_2022_Arch.Microbiol_204_498
PubMedSearch: Saha 2022 Arch.Microbiol 204 498
PubMedID: 35849211
Gene_locus related to this paper: psesp-PURase

Abstract

Polyurethane (PUR) is a soil and aquatic contaminant throughout the world. Towards bioremediation, in a previous study, a soil bacterium, Pseudomonas sp. AKS31, capable of efficiently degrading PUR was isolated. Polyurethanase (PURase) enzyme is capable of cleaving the ester bond of PUR and is considered as a key regulator of PUR biodegradation. Hence, for a high yield, easy purification, and further characterization, the aim of this study was to clone and overexpress the PURase gene of this isolate. The current study also investigated structural aspects of this enzyme through predictive bioinformatics analyses. In this context, the PURase gene of the isolate was cloned and expressed in E. coli using pET28(a)(+) vector. The obtained recombinant protein was found insoluble. Therefore, first, the protein was made soluble with urea and purified using nickel-NTA beads. The purified enzyme exhibited substantial activities when tested on the LA-PUR plate. Bioinformatics-based analysis of the protein revealed the presence of a lipase serine active site and indicated that this PURase belongs to the Family 1.3 lipase. Hence, the present study shows that active PURase can be produced in large quantities using a prokaryotic expression system and thus, provides an effective strategy for in-vitro PUR-degradation.