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Gene_locus Report for: psefl-PULA

Pseudomonas fluorescens polyurethanase lipase A

Relationship
Family|Bacterial_lip_FamI.3
Block| L
Position in NCBI Life Tree|Pseudomonas fluorescens
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Gammaproteobacteria: N E > Pseudomonadales: N E > Pseudomonadaceae: N E > Pseudomonas: N E > Pseudomonas fluorescens group: N E > Pseudomonas fluorescens: N E
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data


Molecular evidence
Database
No mutation
No structure
No kinetic





2 substrates: Impranil, Polyurethane
No inhibitor
2 Genbank : AF144089, AAF66684
1 UniProt : Q9LAB9
1 Interpro : Q9LAB9
1 Pfam : Q9LAB9
1 PIRSF : Q9LAB9
1 SUPERFAM : Q9LAB9
Sequence
Peptide
Graphical view for this peptide sequence: psefl-PULA
Colored MSA for Bacterial_lip_FamI.3 (raw) 
MAITLYSYHNLDNGFAVGYQNNGLGLGLPATLVSALIGGSNAQGVIPGIP
WNPDSEKPALEAVQAAGWTPISASTLGYTARSMHAAPTLAKNSATARRRP
RCWASTMTLATAGNRHQFPRHFRASGKCDHRLHRRCDQRPARRLRAQGLC
QELRGGGFWRVAQRLADTPLPGLGGNDVVVSGHSLGGMAVNSMADLSDST
WSGFYKDANYLAYASPTQSAGDKVLNVGYENDPVFRALDGSSFNLSSLGV
HDKAHESSTDNIVSLNDHYASSLWNVLPFSILNLPTWVSHLPTGYGDGMT
RILDSGFYEQMTRDSTVIVANLSDPARATTWVQDLNRNAEAHKGNTFIIG
SDGDDFIKGGRGADFIEGGKGNDTIRDSSGHNTFLFSGQFGNDRVIGYQA
TDKLVFNDVAGSTDYRDHVKVVGGDTVIGFGTDSVTLVGVSSLSGEGIVI
S
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
MAITLYSYHNLDNGFAVGYQNNGLGLGLPATLVSALIGGSNAQGVIPGIP
WNPDSEKPALEAVQAAGWTPISASTLGYTARSMHAAPTLAKNSATARRRP
RCWASTMTLATAGNRHQFPRHFRASGKCDHRLHRRCDQRPARRLRAQGLC
QELRGGGFWRVAQRLADTPLPGLGGNDVVVSGHSLGGMAVNSMADLSDST
WSGFYKDANYLAYASPTQSAGDKVLNVGYENDPVFRALDGSSFNLSSLGV
HDKAHESSTDNIVSLNDHYASSLWNVLPFSILNLPTWVSHLPTGYGDGMT
RILDSGFYEQMTRDSTVIVANLSDPARATTWVQDLNRNAEAHKGNTFIIG
SDGDDFIKGGRGADFIEGGKGNDTIRDSSGHNTFLFSGQFGNDRVIGYQA
TDKLVFNDVAGSTDYRDHVKVVGGDTVIGFGTDSVTLVGVSSLSGEGIVI
S

References
    Title: Polyurethanases: Three-dimensional structures and molecular dynamics simulations of enzymes that degrade polyurethane
    do Canto VP, Thompson CE, Netz PA
    Ref: J Mol Graph Model, 89:82, 2019 : PubMed

            

    Title: Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens
    Vega RE, Main T, Howard GT
    Ref: International Biodeterioration & Biodegradation, 43:49, 1999 : PubMed

            

    Title: Growth of Pseudomonas fluorescens on a polyester-polyurethane and the purification and characterization of a polyurethanase-protease enzyme
    Howard GT, Blake RC
    Ref: International Biodeterioration & Biodegradation, 42:213, 1998 : PubMed

            


Other Papers

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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