Gene_locus Report for: psef5-q4kbs3

Other strains: Pseudomonas protegens Pf-5

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Gammaproteobacteria: N E > Pseudomonadales: N E > Pseudomonadaceae: N E > Pseudomonas: N E > Pseudomonas fluorescens group: N E > Pseudomonas fluorescens: N E

6_AlphaBeta_hydrolase : psef5-q4k6z9Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta hydrolase fold family, putative, psef5-q4k8j2Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psef5-q4kc21Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) bioh protein, putative, psef5-q4kcq2Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Esterase, putative, psef5-q4kcx3Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psef5-q4ke18Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psef5-q4kf14Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Hydrolase, putative, psef5-q4kfj4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) esterase/lipase/thioesterase family protein, psef5-q4kfw0Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psef5-q4kfw1Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psef5-q4kgg3Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psef5-q4kgs6Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) 3-hydroxyacyl-CoA-acyl carrier protein transferase, psef5-q4kk20Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psef5-q4kk22Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Delta 12 desaturase, psefl-b1pwe2Pseudomonas fluorescens 3-hydroxyacyl-CoA:ACP acyl transferase, psefl-estF1Pseudomonas fluorescens lactone-specific esterase (estf1) gene, psefl-EstXPseudomonas fluorescens EstX, psefl-MUPLPseudomonas fluorescens mupl, psefl-MUPVPseudomonas fluorescens mupv, psefs-c3jzq8Pseudomonas fluorescens (strain SBW25) Conserved hypothetical exported protein, psefs-c3k3d6Pseudomonas fluorescens (strain SBW25) Rhamnosyltransferase 1 subunit A, psefs-c3kdh9Pseudomonas fluorescens (strain SBW25) (R)-3-hydroxydecanoyl-ACP:CoA transacylase, psefs-c3ke34Pseudomonas fluorescens (strain SBW25) Putative family S33 unassigned peptidase, psepf-q3k8g4Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3k8g5Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kdd7Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3ke06Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor, psepf-q3kf33Pseudomonas fluorescens (strain Pf0-1) Putative uncharacterized protein, psepf-q3kfe2Pseudomonas fluorescens (strain PfO-1) lipase, putative, psepf-q3kgd4Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kgm9Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
A85-EsteraseD-FGH : psef5-q4khf1Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) esterase, putative, psepf-q3kh87Pseudomonas fluorescens (strain PfO-1; SBW25) putative esterase.
A85-IroE-IroD-Fes-Yiel : psef5-q4kdb4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) putative esterase superfamily.
ABHD6-Lip : psef5-q4kej0Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta hydrolase fold family , putative, psepf-q3kel6Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor.
ABHD13-BEM46 : psef5-q4kaz4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) bem46 protein, psef5-q4khl0Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) lipoprotein, putative, psepf-q3kew2Pseudomonas fluorescens (strain PfO-1) hypothetical protein precursor, psepf-q3khe9Pseudomonas fluorescens (strain PfO-1) lipoprotein, putative, psefs-c3keb8Pseudomonas fluorescens (strain SBW25). Putative lipoprotein.
abh_upf0017 : psef5-q4k4b4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psefs-c3k3m8Pseudomonas fluorescens (strains SBW25; WH6) Putative uncharacterized protein, psepf-q3k576Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
abh_upf00227 : psef5-q4kj95Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psepf-q3kj14Pseudomonas fluorescens (strain PfO-1) hypothetical protein.
Aclacinomycin-methylesterase_RdmC : psef5-q4k713Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psefs-c3k8y7Pseudomonas fluorescens (strain SBW25) Putative exported hydrolase, psepf-q3k7h4Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor.
AcoC_BiotinLipoyl-ABH : psef5-q4keq4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) (EC 2.3.1.12).
AlphaBeta_hydrolase : psef5-q4kcj3Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) lactone-specific esterase, psef5-q4kcn8Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psepf-q3kd63Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor.
Bacterial_lip_FamI.1 : psef5-q4kj24Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477); Pseudomonas plecoglossicida strain 7 lipase A lipase, psefl-lipaPseudomonas fluorescens lipase(lipA) gene [SIK W1, Genomic, 1595 nt], psepf-q3kbp5Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kiu1Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
Bacterial_lip_FamI.3 : psef5-q4kbs6Pseudomonas protegens (strain Pf-5 / ATCC BAA-477) polyurethanase A PUEA and secreted hemolysin-type RTX family calcium-binding bacteriocin, psefl-LIPAPF33Pseudomonas fluorescens; Pseudomonas sp. AMS8 lipase LipAMS8, extracellular lipase, psefl-lipbPseudomonas fluorescens lipase, psefl-LIPB2Pseudomonas fluorescens lipase, psefl-PULAPseudomonas fluorescens polyurethanase lipase A, psefl-q6gx93Pseudomonas fluorescens ( and strain PfO-1) lipase, psefl-q7wzt7Pseudomonas fluorescens lipase lipJ02, psefl-q76d31Pseudomonas fluorescens lipase (EC 3.1.1.3), psefl-q670w0Pseudomonas fluorescens lipase, psefl-siklipPseudomonas fluorescens gene for triacylglycerol lipase, complete cds, psefl-TLIAPseudomonas fluorescens thermostable lipase tlia, psepf-q3kcs9Pseudomonas fluorescens (strain PfO-1) triacylglycerol lipase (EC 3.1.1.3).
BioH : psef5-q4k4t4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) bioh protein, psefs-c3k362Pseudomonas fluorescens (strain SBW25) Putative biotin biosynthesis-related protein, psepf-q3k5p3Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
CarbLipBact_2 : psef5-q4k717Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Carboxylesterase, psefl-a0a0d0six1Pseudomonas fluorescens. Strain 2-79 PFLU3_contig000018, whole genome shotgun sequence.
Carbon-carbon_bond_hydrolase : psefl-cumD Pseudomonas fluorescens cumD 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase, psefl-NAHNPseudomonas fluorescens 2-hydroxymuconic semialdehyde hydrolase.
Carboxymethylbutenolide_lactonase : psef5-q4kh30Pseudomonas fluorescens; Pseudomonas protegens, 3-oxoadipate enol-lactonase (EC 3.1.1.24), psefl-q6v6x4Pseudomonas fluorescens. Putative hydrolase, psefs-c3kan9Pseudomonas fluorescens (strain SBW25) Putative aromatic-hydrocarbons degradation-related hydrolase, psepf-q3kb84Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kgt9Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase, psefl-a0a075pjy9Pseudomonas fluorescens. 3-oxoadipate enol-lactonase.
Carb_B_Bacteria : psefl-a0a143gh74Pseudomonas fluorescens; Pseudomonas yamanorum. Carboxylesterase, type B.
CFTR-inhibitory-factor_Cif : psef5-q4kax4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) epoxide hydrolase-related protein, psepf-q3kdj7Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor.
Dienelactone_hydrolase : psef5-q4k873Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) dienelactone hydrolase family protein, psef5-q4kfx7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) dienelactone hydrolase family protein, psepf-q3k8c5Pseudomonas fluorescens (strain PfO-1) dienelactone hydrolase precursor, psepf-q3kft7Pseudomonas fluorescens (strain PfO-1) dienelactone hydrolase.
Duf_726 : psefs-c3k8m7Pseudomonas fluorescens (strain SBW25) Putative uncharacterized protein, psepf-q3kcu9Pseudomonas fluorescens (strain Pf0-1) Putative uncharacterized protein.
Duf_900 : psef5-q4kda1Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Putative lipoprotein.
Duf_3530 : psef5-q4k4y2Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Putative uncharacterized protein, psefs-c3k320Pseudomonas fluorescens (strain SBW25) Putative uncharacterized protein, psepf-q3k5t9Pseudomonas fluorescens (strain Pf0-1) Putative uncharacterized protein.
Epoxide_hydrolase : psef5-q4k906Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) 3-oxoadipate enol-lactone hydrolase family protein, psef5-q4k982Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) ephb, putative, psef5-q4kch8Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) epha, psefs-c3k5q5Pseudomonas fluorescens (strain SBW25) Putative hydrolase, psefs-c3kbe5Pseudomonas fluorescens (strain SBW25) Putative epoxide hydrolase, psepf-q3k468Pseudomonas fluorescens (strain PfO-1) epoxide hydrolase-like precursor, psepf-q3k978Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kbr1Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kdm7Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor, psepf-q3ke08Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold precursor, psepf-q3ke09Pseudomonas fluorescens (strain PfO-1) epoxide hydrolase-like precursor, psepf-q3kf76Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
Extracel-MCL-phaZ : psefl-phazPseudomonas fluorescens. Poly(3-hydroxyoctanoate) depolymerase.
Fungal-Bact_LIP : psef5-q4kck4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein.
Haloacetate_dehalogenase : psef5-q4k7i6Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psefs-c3k927Pseudomonas fluorescens (strain SBW25) Putative hydrolase, psepf-q3k995Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
Haloperoxidase : psef5-q4kb21Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) esterase, psefl-cpoF Pseudomonas fluorescens non-heme chloroperoxidase (cpoF), psefl-este Pseudomonas fluorescens SIK WI (and strain SBW25) esterase gene, complete cds, psepf-q3kb51Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kdf3Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3ke91Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
Homoserine_transacetylase : psef5-metxPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477; strain SBW25) homoserine o-acetyltransferase (EC 2.3.1.31) (homoserine transacetylase) (hta), psef5-q4kbd7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psepf-metxPseudomonas fluorescens (strain PfO-1) acetylase) (homoserine transacetylase) (hta).
Hormone-sensitive_lipase_like : psef5-q4k9i3Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) monooxygenase, flavin-binding, psef5-q4kd54Pseudomonas fluorescens; Pseudomonas protegens; Pseudomonas sp. lipase, putative, psepf-q3k919Pseudomonas fluorescens, Pseudomonas sp., esterase/lipase/thioesterase precursor, psepf-q3kca1Pseudomonas fluorescens (strain PfO-1) esterase/lipase/thioesterase, psepf-q3ke24Pseudomonas fluorescens (strain PfO-1) esterase/lipase/thioesterase, psepf-q3keu4Pseudomonas fluorescens (strain PfO-1) esterase/lipase/thioesterase precursor, psepf-q3kje2Pseudomonas fluorescens; Pseudomonas sp.; Pseudomonas putida lipolytic enzyme, psefl-a0a0f4tvz7Pseudomonas fluorescens. Uncharacterized protein, psefl-a0a0f4v6u4Pseudomonas fluorescens Esterase, psefl-a0a0f4te50Pseudomonas fluorescens Esterase, psefl-a0a1b3div8Pseudomonas fluorescens Lipase, psefl-a0a1t2ys16Pseudomonas fluorescens Lipase, psefl-a0a0w0i4l7Pseudomonas fluorescens Lipase, psefl-a0a145x9k4Pseudomonas fluorescens; Pseudomonas yamanorum Lipolytic enzyme, psefl-a0a010rrf3Pseudomonas fluorescens Lipolytic protein, psefl-a0a0n9wuu8Pseudomonas fluorescens Lipase, psefl-a0a0x7k4b6Pseudomonas fluorescens Lipase, psefl-a0a161z1a4Pseudomonas fluorescens Lipase.
Hydrolase_RBBP9_YdeN : psef5-q4kk59Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psepf-q3kju1Pseudomonas fluorescens (strain PfO-1) hypothetical protein.
Lipase_3 : psepf-q3kk13Pseudomonas fluorescens (strain PfO-1) lipase, class 3.
LYsophospholipase_carboxylesterase : psef5-q4k5b5Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psef5-q4keh1Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) phospholipase/carboxylesterase family protein, psef5-q4khv5Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) carboxylesterase (EC 3.1.1.1), psefl-cxes1Pseudomonas fluorescens genes for esterase A, psefl-cxest Pseudomonas fluorescens carboxylesterase EST2 estB, psepf-q3kej7Pseudomonas fluorescens (strain PfO-1) phospholipase/carboxylesterase precursor, psepf-q3khp1Pseudomonas fluorescens (strain PfO-1) phospholipase/carboxylesterase.
MEST-like : psef5-q4ki42Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psefs-c3k1v7Pseudomonas fluorescens (strain SBW25) Putative hydrolase, psepf-q3khw1Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
Monoglyceridelipase_lysophospholip : psef5-q4k3c9Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hypothetical protein, psef5-q4k7u9Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psef5-q4k458Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477; WH6) Putative uncharacterized protein, psefs-c3jz63Pseudomonas fluorescens (strain SBW25; WH6) Putative uncharacterized protein, psefs-c3k3s9Pseudomonas fluorescens (strain SBW25) Putative uncharacterized protein, psepf-q3k524Pseudomonas fluorescens (strain Pf0-1) Putative uncharacterized protein, psepf-q3k812Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
NFM-deformylase : psefs-c3k9x6Pseudomonas fluorescens (strain SBW25). Uncharacterized protein, psefl-a0a0d0tby5Pseudomonas fluorescens. NicD protein.
NLS3-Tex30 : psefs-c3jxp6Pseudomonas fluorescens (strain SBW25) Uncharacterized protein, psepf-q3kf85Pseudomonas fluorescens (strain Pf0-1) Uncharacterized protein, psef5-q4kfd8Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Uncharacterized protein.
PHA_depolymerase_arom : psepf-PHAZPseudomonas fluorescens, Pseudomonas mediterranea, Pseudomonas corrugata, Pseudomonas sp., Comamonas testosteroni (Pseudomonas testosteroni), Pseudomonas chlororaphis (Pseudomonas aureofaciens), Pseudomonas brassicacearum, poly(3-hydroxyalkanoate) depolymerase, psepf-q3kch8Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
PHA_synth_II : psef5-q4kjk5Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) poly(3-hydroxyalkanoate) polymerase 1, psef5-q4kjk7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) poly(3-hydroxyalkanoate) polymerase, psefl-q6xh00Pseudomonas fluorescens phac, psepf-q3kjb8Pseudomonas fluorescens (strain PfO-1) poly(r)-hydroxyalkanoic acid synthase, class II, psepf-q3kjc0Pseudomonas fluorescens (strain PfO-1) poly(r)-hydroxyalkanoic acid synthase, class II.
PMH_Peptidase_S9 : psef5-q4k4u7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) prolyl oligopeptidase family protein, psefl-q32x72Pseudomonas fluorescens dipeptidyl aminopeptidase/acylaminoacyl-peptidase, psepf-q3k5q5Pseudomonas fluorescens (strain PfO-1) peptidase s9, prolyl oligopeptidase active site region.
PolyAspartate-hydrolase : psepf-q3k6f3Pseudomonas fluorescens (strain Pf0-1) Putative exported protein.
Proline_iminopeptidase : psef5-q4k5w4Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) proline iminopeptidase, putative, psef5-q4kjm8Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) proline iminopeptidase (EC 3.4.11.5), psefs-c3k632Pseudomonas fluorescens; Pseudomonas sp. Putative proline iminopeptidase, psefs-laaaPseudomonas fluorescens; Pseudomonas azotoformans; Pseudomonas sp. L-amino acid amidas.e L-proline amide hydrolase, psepf-q3ka37Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold, psepf-q3kje1Pseudomonas fluorescens (strain PfO-1) proline iminopeptidase (pip) (prolyl aminopeptidase) (pap), psefs-c3k813Pseudomonas fluorescens; Pseudomonas synxantha; Pseudomonas sp. Proline iminopeptidase.
RsbQ-like : psefs-c3k7v4Pseudomonas fluorescens (strain SBW25) Putative hydrolase.
S9N_PREPL_Peptidase_S9 : psef5-q4kgj9Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) protease II, psepf-q3kgg4Pseudomonas fluorescens (strain PfO-1) oligopeptidase b (EC 3.4.21.83).
Thioesterase : psef5-q4k989Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) thioesterase, psef5-q4k993Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) non-ribosomal peptide synthase, psef5-q4kay8Pseudomonas fluorescens, pyochelin synthetase f PchF, psef5-q4kaz0Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) pyochelin biosynthetic protein pchc pa4229, psef5-q4kcd3Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) nonribosomal peptide synthase, psef5-q4kes9Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) nonribosomal peptide synthetase, psef5-q4kkf7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) thioesterase, psefl-MMPIIPseudomonas fluorescens MMPII, psefl-PLTGPseudomonas fluorescens putative thioesterase ( and strain Pf-5 / ATCC BAA-477) cfa synthetase, thioesterase component, psefl-Q8RQA8Pseudomonas fluorescens putative thioesterase, psepf-q3kdz6Pseudomonas fluorescens (strain PfO-1) amino acid adenylation, psepf-q3kdz7Pseudomonas fluorescens (strain PfO-1) amino acid adenylation, psepf-q3ke50.1Pseudomonas fluorescens (strain PfO-1) amino acid adenylation, psepf-q3ke50.2Pseudomonas fluorescens (strain PfO-1) amino acid adenylation, psepf-q3kf64Pseudomonas fluorescens (strain PfO-1) amino acid adenylation, psepf-q3kf69Pseudomonas fluorescens (strain PfO-1) thioesterase precursor.
UCP031982 : psef5-q4k5k6Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Putative uncharacterized protein, psef5-q4kc18Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) Lipoprotein, putative, psefs-c3k9z2Pseudomonas fluorescens (strain SBW25) Conserved hypothetical exported protein, psefs-c3k032Pseudomonas fluorescens (strain SBW25) Putative exported protein, psepf-q3kd07Pseudomonas fluorescens (strain Pf0-1) Putative uncharacterized protein.
UCP037442 : psef5-q4kjt7Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) hydrolase, alpha/beta fold family, psefs-c3k6c6Pseudomonas fluorescens (strain SBW25) Putative uncharacterized protein, psepf-q3kjj6Pseudomonas fluorescens (strain PfO-1) alpha/beta hydrolase fold.
VirJ : psef5-q4kha2Pseudomonas fluorescens; Pseudomonas sp.; Pseudomonas protegens, hypothetical protein, psepf-q3kh20Pseudomonas fluorescens; Pseudomonas sp. virulence protein precursor, psefl-a0a075p6j0Pseudomonas fluorescens. Virulence factor family protein, psefl-a0a0b7d7s4Pseudomonas fluorescens; Pseudomonas sp.. Bacterial virulence protein (VirJ), psefl-a0a0c1zqd8Pseudomonas fluorescens; Pseudomonas sp.; Pseudomonas koreensis; Pseudomonas granadensis; Pseudomonas moraviensis. Virulence factor family protein, psefl-a0a0d0pac2Pseudomonas fluorescens. Virulence factor family protein, psefl-a0a0d0tlv6Pseudomonas fluorescens; Pseudomonas sp.; Pseudomonas libanensis; Pseudomonas synxantha. Bacterial virulence protein (VirJ), psefl-a0a0f4t1v9Pseudomonas fluorescens; Pseudomonas sp. Virulence factor family protein, psefl-a0a0f4tn25Pseudomonas fluorescens; Pseudomonas sp.. Virulence factor family protein, psefl-a0a0n9ws51Pseudomonas fluorescens; Pseudomonas sp. Virulence factor family protein, psefl-a0a0x7k5c6Pseudomonas fluorescens. Virulence factor family protein, psefl-a0a143gcb5Pseudomonas fluorescens. Type IV secretory pathway, VirJ component.
Xaa-Pro-like_dom : psefl-a0a143gks7Pseudomonas fluorescens. Putative hydrolase, psefl-a0a0p8xws0Pseudomonas fluorescens. X-Pro dipeptidyl-peptidase family protein

Title: Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5
Hung CS, Zingarelli S, Nadeau LJ, Biffinger JC, Drake CA, Crouch AL, Barlow DE, Russell JN, Jr., Crookes-Goodson WJ
Ref: Applied Environmental Microbiology, 82:6080, 2016 : PubMed
Abstract


ESTHER: Hung_2016_Appl.Environ.Microbiol_82_6080
PubMedSearch: Hung 2016 Appl.Environ.Microbiol 82 6080
PubMedID: 27496773
Gene_locus related to this paper: psef5-q4kbs3, psef5-q4kbs6

Abstract

Polyester polyurethane (PU) coatings are widely used to help protect underlying structural surfaces but are susceptible to biological degradation. PUs are susceptible to degradation by Pseudomonas species, due in part to the degradative activity of secreted hydrolytic enzymes. Microorganisms often respond to environmental cues by secreting enzymes or secondary metabolites to benefit their survival. This study investigated the impact of exposing several Pseudomonas strains to select carbon sources on the degradation of the colloidal polyester polyurethane Impranil DLN (Impranil). The prototypic Pseudomonas protegens strain Pf-5 exhibited Impranil-degrading activities when grown in sodium citrate but not in glucose-containing medium. Glucose also inhibited the induction of Impranil-degrading activity by citrate-fed Pf-5 in a dose-dependent manner. Biochemical and mutational analyses identified two extracellular lipases present in the Pf-5 culture supernatant (PueA and PueB) that were involved in degradation of Impranil. Deletion of the pueA gene reduced Impranil-clearing activities, while pueB deletion exhibited little effect. Removal of both genes was necessary to stop degradation of the polyurethane. Bioinformatic analysis showed that putative Cbr/Hfq/Crc-mediated regulatory elements were present in the intergenic sequences upstream of both pueA and pueB genes. Our results confirmed that both PueA and PueB extracellular enzymes act in concert to degrade Impranil. Furthermore, our data showed that carbon sources in the growth medium directly affected the levels of Impranil-degrading activity but that carbon source effects varied among Pseudomonas strains. This study uncovered an intricate and complicated regulation of P. protegens PU degradation activity controlled by carbon catabolite repression. IMPORTANCE: Polyurethane (PU) coatings are commonly used to protect metals from corrosion. Microbiologically induced PU degradation might pose a substantial problem for the integrity of these coatings. Microorganisms from diverse genera, including pseudomonads, possess the ability to degrade PUs via various means. This work identified two extracellular lipases, PueA and PueB, secreted by P. protegens strain Pf-5, to be responsible for the degradation of a colloidal polyester PU, Impranil. This study also revealed that the expression of the degradative activity by strain Pf-5 is controlled by glucose carbon catabolite repression. Furthermore, this study showed that the Impranil-degrading activity of many other Pseudomonas strains could be influenced by different carbon sources. This work shed light on the carbon source regulation of PU degradation activity among pseudomonads and identified the polyurethane lipases in P. protegens.



        

Title: Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5
Paulsen IT, Press CM, Ravel J, Kobayashi DY, Myers GS, Mavrodi DV, DeBoy RT, Seshadri R, Ren Q and Loper JE <19 more author(s)> Paulsen IT, Press CM, Ravel J, Kobayashi DY, Myers GS, Mavrodi DV, DeBoy RT, Seshadri R, Ren Q, Madupu R, Dodson RJ, Durkin AS, Brinkac LM, Daugherty SC, Sullivan SA, Rosovitz MJ, Gwinn ML, Zhou L, Schneider DJ, Cartinhour SW, Nelson WC, Weidman J, Watkins K, Tran K, Khouri H, Pierson EA, Pierson LS, 3rd, Thomashow LS, Loper JE (- 19)
Ref: Nat Biotechnol, 23:873, 2005 : PubMed
Abstract


ESTHER: Paulsen_2005_Nat.Biotechnol_23_873
PubMedSearch: Paulsen 2005 Nat.Biotechnol 23 873
PubMedID: 15980861
Gene_locus related to this paper: psef5-metx, psef5-q4k3c9, psef5-q4k4b4, psef5-q4k4t4, psef5-q4k4u7, psef5-q4k4y2, psef5-q4k5b5, psef5-q4k5k6, psef5-q4k5w4, psef5-q4k6z9, psef5-q4k7i6, psef5-q4k7u9, psef5-q4k8j2, psef5-q4k9i3, psef5-q4k458, psef5-q4k713, psef5-q4k717, psef5-q4k873, psef5-q4k906, psef5-q4k982, psef5-q4k989, psef5-q4k993, psef5-q4kax4, psef5-q4kay8, psef5-q4kaz0, psef5-q4kaz4, psef5-q4kb21, psef5-q4kbd7, psef5-q4kbs3, psef5-q4kbs6, psef5-q4kc18, psef5-q4kc21, psef5-q4kcd3, psef5-q4kch8, psef5-q4kcj3, psef5-q4kck4, psef5-q4kcn8, psef5-q4kcq2, psef5-q4kcx3, psef5-q4kd54, psef5-q4kda1, psef5-q4kdb4, psef5-q4ke18, psef5-q4keh1, psef5-q4kej0, psef5-q4keq4, psef5-q4kes9, psef5-q4kf14, psef5-q4kfj4, psef5-q4kfw0, psef5-q4kfw1, psef5-q4kfx7, psef5-q4kgg3, psef5-q4kgj9, psef5-q4kgs6, psef5-q4kh30, psef5-q4kha2, psef5-q4khf1, psef5-q4khl0, psef5-q4khv5, psef5-q4ki42, psef5-q4kj24, psef5-q4kj95, psef5-q4kjk5, psef5-q4kjk7, psef5-q4kjm8, psef5-q4kjt7, psef5-q4kk20, psef5-q4kk22, psef5-q4kk59, psef5-q4kkf7, psefl-PLTG, psepf-PHAZ, psef5-q4kfd8

Abstract

Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.