Q8I823 AY162275 (Gatehouse H.S., Gatehouse L.N.) and AF287291 (Nabeshima,T., Kono,Y. and Tomita,T)differ in 2 aa out of 189 overlap and AJ576070 (Javed et al differ in 4 aa from AF28729
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Paraneoptera: NE > Hemiptera: NE > Sternorrhyncha: NE > Aphidomorpha: NE > Aphidoidea: NE > Aphididae: NE > Aphidinae: NE > Macrosiphini: NE > Myzus: NE > Myzus persicae: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSVDCVYTSAVTLLLCCSAVLGRPSSNGGADGGGGGGGGGGAGAGGSGGG GGGGGGGSGVDDTDESPVVVTSSGMVQGYTKIIANREVRVYTGIPFAKPP VGPLRFRRPVAVDPWTGVLNATRLPNTCYQERYEYFPGFVGEEMWNPNTK LSEDCLYLNIWIPKKQRTRHHSNNAHHAKIPVLVWIYGGGYMSGTSTLDI YDGDLLAATFDVMIASMQYRLGAFGSLYLTPELPEDSDDAPGNMGLWDQA LAIKWIKENAAAFGADPETITLFGESAGGGSVSVHLISPETRGMVRRGII QSGTVNAPWSYMTGERAVDIAKKLLDDCNCNSTTLDSNPIATMSCMRAVD ASTISKKQWNNYSGILGFPSAPTVDGVLLPEHPLDMLAKANFSDIDILIG SNLNEGTYFLLYDFVDFFDRTSATALPKEKFVQIVNVIFKDKTQLERDAI IYQYSGWEKKDVDDKYSNQKQLSDVVADYFFVCPTNLFANIVSSRGARVY YYFFTHRTDSHLWGEWMGVLHGDEMQYVFGHPLNMSMPYNARERDLSIRI MEAFTRFSLTGTPVSDDIDWPLYNESNPIYHVWNAAELHVGYGPRAAECQ FWNGFFPKIAQALKETSKITCEDYPDSMPTTNENCTFTSSFASINPQISF AIIFIFVLPAHGLL
References
Title: Characterization of acetylcholinesterases, and their genes, from the hemipteran species Myzus persicae (Sulzer), Aphis gossypii (Glover), Bemisia tabaci (Gennadius) and Trialeurodes vaporariorum (Westwood) Javed N, Viner R, Williamson MS, Field LM, Devonshire AL, Moores GD Ref: Insect Molecular Biology, 12:613, 2003 : PubMed
Gene sequences encoding putative acetylcholinesterases have been reported for four hemipteran insect species. Although acetylcholinesterase insensitivity occurs in insecticide-resistant populations of each of these species, no mutations were detected in the gene sequences from the resistant insects. This, coupled with a series of experiments using novel reversible inhibitors to compare the biochemical characteristics of acetylcholinesterase from a range of insect species, showed that the cloned cDNA fragments are unlikely to encode the hemipteran synaptic acetylcholinesterases, and there is likely to be a second ace locus.
Title: An amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach potato aphid, Myzus persicae Nabeshima T, Kozaki T, Tomita T, Kono Y Ref: Biochemical & Biophysical Research Communications, 307:15, 2003 : PubMed
cDNAs encoding two acetylcholinesterases (AChEs) were isolated from the peach potato aphid, Myzus persicae. MpAChE1 was orthologous and MpAChE2 was paralogous with the ace of Drosophila melanogaster. The deduced amino acid sequence of MpAChE1 cDNA was identical between the pirimicarb susceptible and resistant strains. However, a single amino acid substitution of Ser431Phe on MpAchE2 was found in the pirimicarb resistant strains. This substitution was located in the acyl pocket of the enzyme and was thought to alter the ligand specificity.
