(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Mammalia: NE > Theria: NE > Eutheria: NE > Boreoeutheria: NE > Euarchontoglires: NE > Glires: NE > Rodentia: NE > Myomorpha: NE > Muroidea: NE > Muridae: NE > Murinae: NE > Mus [genus]: NE > Mus [subgenus]: NE > Mus musculus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKQEQQPTPGARATQSQPADQELGSNSPPQRNWKGIAIALLVILVVCSLI TMSVILLTPDELTNSSETRLSLEELLGKGFGLHNPEPRWINDTVVVYKTN NGHVMKLNTESNASTLLLDNSTFVTFKASRHSLSPDLKYVLLAYDVKQIF HYSFTASYLIYNIHTGEVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENN IYYQPDIKSSSLRLTSSGKEGIIFNGIADWLYEEELLHSHIAHWWSPDGE RLAFLMINDSLVPNMIIPRFTGALYPKAKQYPYPKAGQANPSVKLYVVNL YGPTHTLELMPPDIFKSREYYITMVKWVSNTRTVVRWLNRPQNISILTLC ESTTGACSRKYEMTSDTWLSKQNEEPVFSRDGSKFFMTVPVKQGGRGEFH HIAMFLVQSKSEQITVRHLTSGNWEVIRILAYDETTQKIYFLSTESSPQG RQLYSASTEGLLNRDCISCNFMKEDCTYFDASFSPMNQHFLLFCEGPKVP VVSLHITDNPSRYFLLENNSVMKETIQKKKLAKRETRILHIDDYELPLQL SFPKDFMEKNQYALLLIMDEEPGGQMVTDKFHVDWDSVLIDTDNVIVARF DGRGSGFQGLKVLQEIHRRIGSVEAKDQVAAVKYLLKQPYIDSKRLSIFG KGYGGYIASMILKSDEKFFKCGAVVAPISDMKLYASAFSERYLGMPSKEE STYQASSVLHNIHGLKEENLLIIHGTADTKVHFQHSAELIKHLIKAGVNY TLQVYPDEGYHISDKSKHHFYSTILRFFSDCLKEEVSVLPQEPEEDE
The mouse (Mus musculus) is the premier animal model for understanding human disease and development. Here we show that a comprehensive understanding of mouse biology is only possible with the availability of a finished, high-quality genome assembly. The finished clone-based assembly of the mouse strain C57BL/6J reported here has over 175,000 fewer gaps and over 139 Mb more of novel sequence, compared with the earlier MGSCv3 draft genome assembly. In a comprehensive analysis of this revised genome sequence, we are now able to define 20,210 protein-coding genes, over a thousand more than predicted in the human genome (19,042 genes). In addition, we identified 439 long, non-protein-coding RNAs with evidence for transcribed orthologs in human. We analyzed the complex and repetitive landscape of 267 Mb of sequence that was missing or misassembled in the previously published assembly, and we provide insights into the reasons for its resistance to sequencing and assembly by whole-genome shotgun approaches. Duplicated regions within newly assembled sequence tend to be of more recent ancestry than duplicates in the published draft, correcting our initial understanding of recent evolution on the mouse lineage. These duplicates appear to be largely composed of sequence regions containing transposable elements and duplicated protein-coding genes; of these, some may be fixed in the mouse population, but at least 40% of segmentally duplicated sequences are copy number variable even among laboratory mouse strains. Mouse lineage-specific regions contain 3,767 genes drawn mainly from rapidly-changing gene families associated with reproductive functions. The finished mouse genome assembly, therefore, greatly improves our understanding of rodent-specific biology and allows the delineation of ancestral biological functions that are shared with human from derived functions that are not.
Title: Cloning and characterization of dipeptidyl peptidase 10, a new member of an emerging subgroup of serine proteases Qi SY, Riviere PJ, Trojnar J, Junien JL, Akinsanya KO Ref: Biochemical Journal, 373:179, 2003 : PubMed
Two dipeptidyl peptidase IV (DPPIV, DPP4)-related proteins, DPP8 and DPP9, have been identified recently [Abbott, Yu, Woollatt, Sutherland, McCaughan, and Gorrell (2000) Eur. J. Biochem. 267, 6140-6150; Olsen and Wagtmann (2002) Gene 299, 185-193; Qi, Akinsanya, Riviere, and Junien (2002) Patent application WO0231134]. In the present study, we describe the cloning of DPP10, a novel 796-amino-acid protein, with significant sequence identity to DPP4 (32%) and DPP6 (51%) respectively. We propose that DPP10 is a new member of the S9B serine proteases subfamily. The DPP10 gene is located on the long arm of chromosome 2 (2q12.3-2q14.2), close to the DPP4 (2q24.3) and FAP (2q23) genes. The active-site serine residue is replaced by a glycine residue in DPP10, resulting in the loss of DPP activity. The serine residue is also replaced in DPP6, which lacks peptidase activity. DPP8 and DPP9 share an identical active site with DPP4 (Gly-Trp-Ser-Tyr-Gly). In contrast with the previous results suggesting that DPP9 is inactive, we show that DPP9 is a DPP, hydrolysing Ala-Pro-(7-amino-4-methyl-coumarin) with similar pH-specificity and protease-inhibitor-sensitivity to those of DPP4 and DPP8. Northern-blot analysis shows that whereas DPP8 and DPP9 are widely expressed, DPP10 is expressed mainly in the brain and pancreas. DPP6, which has the highest amino acid identity with DPP10, has been shown previously [Nadal, Ozaita, Amarillo, de Miera, Ma, Mo, Goldberg, Misumi, Ikehara, Neubert et al. (2003) Neuron 37, 449-461] to associate with A-type K(+) channel subunits, modulating their transport and function in somatodendritic compartments of neurons. It is possible that DPP10 is involved in similar functions in the brain. Elucidation of the physiological or pathophysiological role of DPP8, DPP9 and DPP10 and characterization of their structure-function relationships will add impetus to the development of inhibitor molecules for pharmacological or therapeutic use.
Mus musculus (Mouse) Abhydrolase domain-containing protein 10, mitochondrial