Gene_locus Report for: human-ACOT1 Homo sapiens (Human) Inducible cytosolic acyl-coenzyme A thioester hydrolase Long chain Acyl-CoA hydrolase) (cte-i) (cte-ib)
Comment
The mouse ACOT gene cluster comprises six genes with localizations in cytosol (ACOT1), mitochondria (ACOT2), and peroxisomes (ACOT3-6). The corresponding human gene cluster contains only three genes (ACOT1, ACOT2, and ACOT4) coding for full-length thioesterase proteins only ACOT4 is peroxisomal Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Deuterostomia: N E > Chordata: N E > Craniata: N E > Vertebrata: N E > Gnathostomata: N E > Teleostomi: N E > Euteleostomi: N E > Sarcopterygii: N E > Dipnotetrapodomorpha: N E > Tetrapoda: N E > Amniota: N E > Mammalia: N E > Theria: N E > Eutheria: N E > Boreoeutheria: N E > Euarchontoglires: N E > Primates: N E > Haplorrhini: N E > Simiiformes: N E > Catarrhini: N E > Hominoidea: N E > Hominidae: N E > Homininae: N E > Homo: N E > Homo sapiens: N E A85-EsteraseD-FGH :
human-ESD .
ABHD6-Lip :
human-ABHD6 .
ABHD8 :
human-ABHD8 Homo sapiens (Human) Abhydrolase domain containing 8 (ABHD8) cDNA FLJ11743 fis, clone HEMBA1005517 .
ABHD10 :
human-ABHD10 Homo sapiens (Human) ABHDA ABHD10 Abhydrolase domain-containing protein 10, Mycophenolic acid acyl-glucuronide esterase, mitochondrial .
ABHD11-Acetyl_transferase :
human-ABHD11 Homo sapiens (Human) (EC 3.3.2.3) Abhydrolase domain-containing protein 11 williams-beuren syndrome critical region protein 21 .
ABHD12-PHARC :
human-ABHD12 Homo sapiens (Human) abhydrolase domain-containing protein 12. Protein C20orf22, flj90542, CT022, 2-arachidonoylglycerol hydrolase, Monoacylglycerol lipase ,
human-ABHD12B Homo sapiens (Human) Abhydrolase domain-containing protein 12B ABHD12B protein c14orf29 .
ABHD13-BEM46 :
human-ABHD13 Homo sapiens (Human) C13orf6 Q7L211 ABHDD_HUMAN ABHD13 Abhydrolase domain-containing protein 13 .
ABHD16 :
human-ABHD16A Homo sapiens (Human) Abhydrolase domain-containing protein 16A BAT5 (HLA-B-associated transcript 5) (NG26 protein) (G5) (PP199) ,
human-ABHD16B Homo sapiens (Human) ABHD16B PS-PLA1 lipase activity .
ABHD17-depalmitoylase :
human-ABHD17A Homo sapiens (Human) Abhydrolase domain-containing protein FAM108A1, C19orf27 ABHD17A ,
human-ABHD17B Homo sapiens (Human) CGI-67 C9orf77 FAM108B1 protein Abhydrolase domain-containing protein FAM108B1 ,
human-ABHD17C Homo sapiens (Human) Abhydrolase domain-containing protein FAM108C1 Q6PCB6 F108C_HUMAN .
ABHD18 :
human-ABHD18 Homo sapiens (Human) ABHD18 C4orf29 CD029 hypothetical protein .
abh_upf0017 :
human-ABHD1 Homo sapiens (Human) lung alpha/beta hydrolase 1 ,
human-ABHD2 Homo sapiens (Human) Monoacylglycerol lipase ABHD2 LABH2 LBH2 protein phps1-2 ,
human-ABHD3 Homo sapiens (Human) hypothetical 49.3 kda protein ,
human-ABHD15 Homo sapiens (Human) ABH15 Abhydrolase domain-containing protein 15 .
ACHE :
human-ACHE .
Acidic_Lipase :
human-LIPA ,
human-LIPF ,
human-LIPJ Homo sapiens (Human) Lipase member J lipase-like, ab-hydrolase domain containing 1 ,
human-LIPK Homo sapiens (Human) Lipase member K lipase-like, ab-hydrolase domain containing 2 LIPL2 ,
human-LIPM Homo sapiens (Human) LIPM LIPL3 ba304i5.1 ,
human-LIPN Homo sapiens (Human) lipase-like, Lipase-like abhydrolase domain-containing protein 4 .
ACPH_Peptidase_S9 :
human-APEH Homo sapiens (Human) acylamino acid-releasing enzyme APH APEH .
Acyl-CoA_Thioesterase :
human-ACOT2 ,
human-ACOT4 ,
human-ACOT6 Homo sapiens (Human) Acyl-CoA thioesterase 6 (EC 3.1.2.2) ,
human-BAAT Homo sapiens (Human) bile acid CoA: amino acid n-acyltransferase (EC 3.1.2.2) .
Arb2_FAM172A :
human-f172a Homo sapiens (Human).Cotranscriptional regulator Protein FAM172A .
Arylacetamide_deacetylase :
human-AADAC Homo sapiens (Human) arylacetamide deacetylase ,
human-AADACL2 Homo sapiens (Human) similar to arylacetamide deacetylase (aadac) ,
human-AADACL3 Homo sapiens (Human) AADACL3 arylacetamide deacetylase-like 3 ADCL3 ,
human-AADACL4 Homo sapiens (Human) Arylacetamide deacetylase-like 4 ,
human-NCEH1 Homo sapiens (Human) NCEH1 KIAA1363 AADACL1 neutral cholesterol ester hydrolase 1 .
BCHE :
human-BCHE .
Carboxypeptidase_S10 :
human-CPVL Homo sapiens (Human) carboxypeptidase, vitellogenic-like CP-Mac ou CPVL carboxypeptidase WUG ,
human-CTSA ,
human-SCPEP1 Homo sapiens (Human) serine Retinoid-inducible serine carboxypeptidase RISC SCP1 (EC 3.4.16.-) .
Carb_B_Chordata :
human-CES1 ,
human-CES2 Homo sapiens (Human) carboxylesterase hCE-2,iCE, hiCE, CES2 gene cDNA FLJ76104 Cocaine esterase ,
human-CES3 Homo sapiens (Human) Carboxylesterase 3 (Brain) Liver carboxylesterase 31 homolog ,
human-CES4A Homo sapiens (Human) Carboxylesterase 4A Carboxylesterase 8 ,
human-CES5A Homo sapiens (Human) est5a CES7 Cauxin Carboxylesterase-like urinary excreted protein homolog .
CGI-58_ABHD5_ABHD4 :
human-ABHD4 Homo sapiens (Human) abhydrolase domain-containing protein 4 FLJ12816 similar to 2-hydroxymuconic semialdehyde hydrolase (EC 3.1.1.-) ,
human-ABHD5 .
Cholesterol_esterase :
human-CEL .
CIB-CCG1-interacting-factor-B :
human-ABHD14A Homo sapiens (Human) Abhydrolase domain-containing protein 14A srsq1913 ,
human-CIB .
CMBL :
human-CMBL Homo sapiens (Human) Carboxymethylenebutenolidase homolog .
DPP4N_Peptidase_S9 :
human-DPP4 ,
human-DPP6 ,
human-DPP8 ,
human-DPP9 ,
human-DPP10 ,
human-FAP .
Duf_676 :
human-FAM135A Homo sapiens (Human) F135A DKFZp781H2319 FLJ20176 fis KIAA1411 previously human-F135A ,
human-FAM135B Homo sapiens (Human) F135B loc51059 c8orfk32 protein .
Duf_726 :
human-TMCO4 Homo sapiens (Human) Transmembrane and coiled-coil domain-containing protein 4 .
Duf_829 :
human-TMEM53 Homo sapiens (Human) Transmembrane protein 53, FLJ22353, NET4 .
Epoxide_hydrolase :
human-EPHX1 Homo sapiens (Human) microsomal epoxide hydrolase HYEP mEH, epoxide hydratase EPHX1 ,
human-EPHX2 ,
human-EPHX3 Homo sapiens (Human) Epoxide hydrolase 3 (EPHX3) Abhydrolase domain-containing protein 9 (ABHD9) FLJ22408 ,
human-EPHX4 Homo sapiens (Human) Epoxide hydrolase 4 EPHX4 ABHD7 EPHXRP Abhydrolase domain-containing protein 7 .
FSH1 :
human-OVCA2 Homo sapiens (Human) Candidate tumor suppressor in ovarian cancer .
Hepatic_Lipase :
human-LIPC Homo sapiens (Human) LIPC hepatic triacylglycerol lipase HTGL .
Hormone-sensitive_lipase_like :
human-LIPE Human mRNA (Human) hormone sensitive lipase HSL .
Hydrolase_RBBP9_YdeN :
human-RBBP9 .
Kynurenine-formamidase :
human-AFMID Homo sapiens (Human) Kynurenine formamidase .
LIDHydrolase :
human-LDAH Homo sapiens (Human) lipid droplet-associated hydrolase (LDAH) C2orf43 .
Lipase_3 :
human-DAGLA Homo sapiens (Human) DAGLA Sn1-specific diacylglycerol lipase alpha DGL-alpha, neural stem cell-derived dendrite regulator KIAA0659 ,
human-DAGLB Homo sapiens (Human) DAGLB Sn1-specific diacylglycerol lipase beta kccr13l FLJ36639 .
Lipoprotein_Lipase :
human-LIPG Homo sapiens (Human) endothelial lipase LIPE_HUMAN flj43354 ,
human-LPL .
LYsophospholipase_carboxylesterase :
human-LYPLA1 ,
human-LYPLA2 ,
human-LYPLAL1 .
Maspardin-ACP33-SPG21_like :
human-SPG21 Homo sapiens (Human) Maspardin spg21 acid cluster protein 33 ACP33 sbm-019 (gl010)flj24010 Maspardin .
MEST-like :
human-MEST Homo sapiens (Human) MEST mesoderm-specific transcript .
Monoglyceridelipase_lysophospholip :
human-MGLL .
Ndr_family :
human-NDRG1 ,
human-NDRG2 ,
human-NDRG3 ,
human-NDRG4 Homo sapiens (Human) NDRG4, N-myc downstream-regulated gene 4 protein (smap-8) flj42011 flj16174 flj44611 .
Neuroligin :
human-NLGN1 ,
human-NLGN2 ,
human-NLGN3 Homo sapiens (Human) Neuroligin 3 KIAA1480 ,
human-NLGN4X ,
human-NLGN4Y Homo sapiens (Human) Neuroligin-4, Y-linked precursor (Neuroligin Y) KIAA0951 .
NLS3-Tex30 :
human-KANSL3 Homo sapiens (Human) KAT8 regulatory NSL complex subunit 3, Testis development protein PRTD, KIAA1310, PRTD, SI1, FLJ10081, NSL3, Rcd1 ,
human-TEX30 Homo sapiens (Human) testis expressed 30 C13orf27 chromosome 13 open reading frame 27 .
PAF-Acetylhydrolase :
human-PAFAH2 Homo sapiens (Human) (EC 3.1.1.47) platelet-activating factor acetylhydrolase 2, cytoplasmic (serine dependent phospholipase a2) (hsd-pla2), PAFAH2, PAFA2 PAF-AH ,
human-PLA2G7 .
Palmitoyl-protein_thioesterase :
human-PPT1 ,
human-PPT2 .
Pancreatic_lipase :
human-PNLIP ,
human-PNLIPRP1 ,
human-PNLIPRP2 ,
human-PNLIPRP3 Homo sapiens (Human) Pancreatic lipase-related protein 3 .
PC-sterol_acyltransferase :
human-LCAT ,
human-PLA2G15 .
Pectinacetylesterase-Notum :
human-NOTUM .
PGAP1 :
human-PGAP1 Homo sapiens (Human)GPI inositol-deacylase PGAP1 117.8 kd protein in ste2-frs2 intergenic region ,
human-SERAC1 Homo sapiens (Human) Protein SERAC1 .
Phospholipase :
human-LIPH Homo sapiens (Human) membrane-bound phosphatidic acid-selective phospholipase a1-alpha, LPD lipase-related protein mPA-PLA1 alpha ,
human-LIPI Homo sapiens (Human) membrane-associated phosphatidic acid-selective phospholipase a1 beta mPA-PLA1 beta (LPD lipase) Cancer/testis antigen 17 CT17 ,
human-PLA1A Homo sapiens (Human) Phospholipase A1 member A, phosphatidylserine-specific phospholipase A1 deltaC .
PPase_methylesterase_euk :
human-PPME1 .
Prolylcarboxypeptidase :
human-DPP7 ,
human-PRCP ,
human-PRSS16 Homo sapiens (Human) PRSS16 protease, serine, 16 (thymus) TSSP thymus-specific serine protease precursor (EC 3.4.-.-) .
S9N_PPCE_Peptidase_S9 :
human-PREP .
S9N_PREPL_Peptidase_S9 :
human-PREPL .
SERHL :
human-SERHL2 Homo sapiens (Human) serine hydrolase-like protein 2 SERHL2 chomosome 22 .
Thioesterase :
human-FASN ,
human-OLAH .
Thyroglobulin :
human-TG .
Valacyclovir-hydrolase :
human-BPHL Molecular evidence
Database
Sequence
Graphical view for this peptide sequence: human-ACOT1 Colored MSA for Acyl-CoA_Thioesterase (raw)
MAATLILEPAGRCCWDEPVRIAVRGLAPEQPVTLRASLRDEKGALFQAHA
RYRADTLGELDLERAPALGGSFAGLEPMGLLWALEPEKPLVRLVKRDVRT
PLAVELEVLDGHDPDPGRLLCRVRHERYFLPPGVRREPVRAGRVRGTLFL
PPEPGPFPGIVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTM
ETLHLEYFEEAVNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITA
AVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPL
EGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKP
QIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHAMAQVDAWK
QLQTFFHKHLGGHEGTIPSKV
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M A A T L I L E P A G R C C W D E P V R I A V R G L A P E Q P V T L R A S L R D E K G A L F Q A H A R Y R A D T L G E L D L E R A P A L G G S F A G L E P M G L L W A L E P E K P L V R L V K R D V R T P L A V E L E V L D G H D P D P G R L L C R V R H E R Y F L P P G V R R E P V R A G R V R G T L F L P P E P G P F P G I V D M F G T G G G L L E Y R A S L L A G K G F A V M A L A Y Y N Y E D L P K T M E T L H L E Y F E E A V N Y L L S H P E V K G P G V G L L G I S K G G E L C L S M A S F L K G I T A A V V I N G S V A N V G G T L R Y K G E T L P P V G V N R N R I K V T K D G Y A D I V D V L N S P L E G P D Q K S F I P V E R A E S T F L F L V G Q D D H N W K S E F Y A N E A C K R L Q A H G R R K P Q I I C Y P E T G H Y I E P P Y F P L C R A S L H A L V G S P I I W G G E P R A H A M A Q V D A W K Q L Q T F F H K H L G G H E G T I P S K V
References
Title: Remodeling natural products: chemistry and serine hydrolase activity of a rocaglate-derived beta-lactoneLajkiewicz NJ , Cognetta AB, 3rd , Niphakis MJ , Cravatt BF , Porco JA, Jr. Ref: Journal of the American Chemical Society, 136 :2659, 2014 : PubMed Abstract ESTHER: Lajkiewicz_2014_J.Am.Chem.Soc_136_2659 PubMedSearch: Lajkiewicz 2014 J.Am.Chem.Soc 136 2659 PubMedID: 24447064 Gene_locus related to this paper: human-ABHD10 ,
human-ACOT1 ,
human-ACOT2 ,
human-CTSA ,
human-SCPEP1 ,
mouse-abhda Inhibitor(s) related to this paper: Rocaglate-Derived-beta-Lactone-5 Abstract
Flavaglines are a class of natural products with potent insecticidal and anticancer activities. beta-Lactones are a privileged structural motif found in both therapeutic agents and chemical probes. Herein, we report the synthesis, unexpected light-driven di-epimerization, and activity-based protein profiling of a novel rocaglate-derived beta-lactone. In addition to in vitro inhibition of the serine hydrolases ABHD10 and ACOT1/2, the most potent beta-lactone enantiomer was also found to inhibit these enzymes, as well as the serine peptidases CTSA and SCPEP1, in PC3 cells.
Title: Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTsHunt MC , Rautanen A , Westin MA , Svensson LT , Alexson SE Ref: FASEB Journal, 20 :1855, 2006 : PubMed Abstract ESTHER: Hunt_2006_FASEB.J_20_1855 PubMedSearch: Hunt 2006 FASEB.J 20 1855 PubMedID: 16940157 Gene_locus related to this paper: human-ACOT1 ,
human-ACOT2 ,
human-ACOT4 ,
human-ACOT6 ,
mouse-acot1 ,
mouse-acot2 ,
mouse-acot3 ,
mouse-acot4 ,
mouse-acot5 ,
mouse-ACOT6 Abstract
The maintenance of cellular levels of free fatty acids and acyl-CoAs, the activated form of free fatty acids, is extremely important, as imbalances in lipid metabolism have serious consequences for human health. Acyl-coenzyme A (CoA) thioesterases (ACOTs) hydrolyze acyl-CoAs to the free fatty acid and CoASH, and thereby have the potential to regulate intracellular levels of these compounds. We previously identified and characterized a mouse ACOT gene cluster comprised of six genes that apparently arose by gene duplications encoding acyl-CoA thioesterases with localizations in cytosol (ACOT1), mitochondria (ACOT2), and peroxisomes (ACOT3-6). However, the corresponding human gene cluster contains only three genes (ACOT1, ACOT2, and ACOT4) coding for full-length thioesterase proteins, of which only one is peroxisomal (ACOT4). We therefore set out to characterize the human genes, and we show here that the human ACOT4 protein catalyzes the activities of three mouse peroxisomal ACOTs (ACOT3, 4, and 5), being active on succinyl-CoA and medium to long chain acyl-CoAs, while ACOT1 and ACOT2 carry out similar functions to the corresponding mouse genes. These data strongly suggest that the human ACOT4 gene has acquired the functions of three mouse genes by a functional convergent evolution that also provides an explanation for the unexpectedly low number of human genes.
Title: Complete sequencing and characterization of 21,243 full-length human cDNAsOta T , Suzuki Y , Nishikawa T , Otsuki T , Sugiyama T , Irie R , Wakamatsu A , Hayashi K , Sato H and Sugano S <144 more author(s)> Ota T , Suzuki Y , Nishikawa T , Otsuki T , Sugiyama T , Irie R , Wakamatsu A , Hayashi K , Sato H , Nagai K , Kimura K , Makita H , Sekine M , Obayashi M , Nishi T , Shibahara T , Tanaka T , Ishii S , Yamamoto J , Saito K , Kawai Y , Isono Y , Nakamura Y , Nagahari K , Murakami K , Yasuda T , Iwayanagi T , Wagatsuma M , Shiratori A , Sudo H , Hosoiri T , Kaku Y , Kodaira H , Kondo H , Sugawara M , Takahashi M , Kanda K , Yokoi T , Furuya T , Kikkawa E , Omura Y , Abe K , Kamihara K , Katsuta N , Sato K , Tanikawa M , Yamazaki M , Ninomiya K , Ishibashi T , Yamashita H , Murakawa K , Fujimori K , Tanai H , Kimata M , Watanabe M , Hiraoka S , Chiba Y , Ishida S , Ono Y , Takiguchi S , Watanabe S , Yosida M , Hotuta T , Kusano J , Kanehori K , Takahashi-Fujii A , Hara H , Tanase TO , Nomura Y , Togiya S , Komai F , Hara R , Takeuchi K , Arita M , Imose N , Musashino K , Yuuki H , Oshima A , Sasaki N , Aotsuka S , Yoshikawa Y , Matsunawa H , Ichihara T , Shiohata N , Sano S , Moriya S , Momiyama H , Satoh N , Takami S , Terashima Y , Suzuki O , Nakagawa S , Senoh A , Mizoguchi H , Goto Y , Shimizu F , Wakebe H , Hishigaki H , Watanabe T , Sugiyama A , Takemoto M , Kawakami B , Watanabe K , Kumagai A , Itakura S , Fukuzumi Y , Fujimori Y , Komiyama M , Tashiro H , Tanigami A , Fujiwara T , Ono T , Yamada K , Fujii Y , Ozaki K , Hirao M , Ohmori Y , Kawabata A , Hikiji T , Kobatake N , Inagaki H , Ikema Y , Okamoto S , Okitani R , Kawakami T , Noguchi S , Itoh T , Shigeta K , Senba T , Matsumura K , Nakajima Y , Mizuno T , Morinaga M , Sasaki M , Togashi T , Oyama M , Hata H , Komatsu T , Mizushima-Sugano J , Satoh T , Shirai Y , Takahashi Y , Nakagawa K , Okumura K , Nagase T , Nomura N , Kikuchi H , Masuho Y , Yamashita R , Nakai K , Yada T , Ohara O , Isogai T , Sugano S (- 144) Ref: Nat Genet, 36 :40, 2004 : PubMed Abstract ESTHER: Ota_2004_Nat.Genet_36_40 PubMedSearch: Ota 2004 Nat.Genet 36 40 PubMedID: 14702039 Gene_locus related to this paper: human-ABHD1 ,
human-ABHD4 ,
human-ABHD12 ,
human-ABHD16A ,
human-ACOT1 ,
human-LDAH ,
human-ABHD18 ,
human-CES1 ,
human-CES4A ,
human-CES5A ,
human-CPVL ,
human-DAGLB ,
human-EPHX2 ,
human-KANSL3 ,
human-LIPA ,
human-LPL ,
human-MEST ,
human-NDRG1 ,
human-NLGN1 ,
human-NLGN4X ,
human-PRCP ,
human-PRSS16 ,
human-SERAC1 ,
human-TMEM53 Abstract
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.
Other Papers
Homo sapiens (Human) esterase D (EC 3.1.1.1) formylglutathione hydrolase