Gene_locus Report for: helam-ACHE

Wee,C.W. and Heckel,D.G. AY142325 and Q68J52 Rao N.G.V., Nimbalkar S.A., Satpute U.S. aa diff from AF369793 by Ren,X., Han,Z. and Wang,Y

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Protostomia: N E > Ecdysozoa: N E > Panarthropoda: N E > Arthropoda: N E > Mandibulata: N E > Pancrustacea: N E > Hexapoda: N E > Insecta: N E > Dicondylia: N E > Pterygota: N E > Neoptera: N E > Holometabola: N E > Amphiesmenoptera: N E > Lepidoptera: N E > Glossata: N E > Neolepidoptera: N E > Heteroneura: N E > Ditrysia: N E > Obtectomera: N E > Noctuoidea: N E > Noctuidae: N E > Heliothinae: N E > Helicoverpa: N E > Helicoverpa armigera: N E

Title: Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hubner)
Ren X, Han Z, Wang Y
Ref: Archives of Insect Biochemistry & Physiology, 51:103, 2002 : PubMed
Abstract


ESTHER: Ren_2002_Arch.Insect.Biochem.Physiol_51_103
PubMedSearch: Ren 2002 Arch.Insect.Biochem.Physiol 51 103
PubMedID: 12386838
Gene_locus related to this paper: helam-ACHE

Abstract

Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5' untranslated regions (UTR) of 315 bp and 3' UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos.