(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Mammalia: NE > Theria: NE > Eutheria: NE > Boreoeutheria: NE > Laurasiatheria: NE > Carnivora: NE > Feliformia: NE > Felidae: NE > Felinae: NE > Felis: NE > Felis catus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRPPWCPLYTPSLAAPILLLLLFLLGGGAEAEDPELLVTVRGGQLRGVRL MAPGGPVSAFLGIPFAEPPVGPRRFLPPEPKRPWPGVLDATAFQSVCYQY VDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLVWIYGGG FYSGASSLDVYDGRFLAQAEGTVLVSMNYRVGAFGFLALPGSREAPGNVG LLDQRLALQWVQDNVATFGGDPMSVTLFGESAGAASVGMHLLSPPSRGLF HRAVLQSGAPNGPWATVGVGEARRRATLLARLVGCPPGGAGGNDTELVAC LRTRPAQDLVDHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFH GLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAQFLAGVRVGVPQASD LAAEAVVLHYTDWLNPEDPARLREAMSDVVGDHNVVCPVAQLAGRLAAQG ARVYAYIFEHRASTLSWPLWMGVPHGYEIEFIFGLPLEPSLNYTAEERIF AQRLMRYWANFARTGDPNDPRDPKVPQWPPYTAGAQQYVSLDLRPLEVRR GLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFD HYSKQDRCSDL
The genome sequence (1.9-fold coverage) of an inbred Abyssinian domestic cat was assembled, mapped, and annotated with a comparative approach that involved cross-reference to annotated genome assemblies of six mammals (human, chimpanzee, mouse, rat, dog, and cow). The results resolved chromosomal positions for 663,480 contigs, 20,285 putative feline gene orthologs, and 133,499 conserved sequence blocks (CSBs). Additional annotated features include repetitive elements, endogenous retroviral sequences, nuclear mitochondrial (numt) sequences, micro-RNAs, and evolutionary breakpoints that suggest historic balancing of translocation and inversion incidences in distinct mammalian lineages. Large numbers of single nucleotide polymorphisms (SNPs), deletion insertion polymorphisms (DIPs), and short tandem repeats (STRs), suitable for linkage or association studies were characterized in the context of long stretches of chromosome homozygosity. In spite of the light coverage capturing approximately 65% of euchromatin sequence from the cat genome, these comparative insights shed new light on the tempo and mode of gene/genome evolution in mammals, promise several research applications for the cat, and also illustrate that a comparative approach using more deeply covered mammals provides an informative, preliminary annotation of a light (1.9-fold) coverage mammal genome sequence.
Title: Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma Bartels CF, Xie W, Miller-Lindholm AK, Schopfer LM, Lockridge O Ref: Biochemical Pharmacology, 60:479, 2000 : PubMed
Cat serum contains 0.5 mg/L of butyrylcholinesterase (BChE, EC 3.1.1. 8) and 0.3 mg/L of acetylcholinesterase (AChE, EC 3.1.1.7); this can be compared with 5 mg/mL and < 0.01 mg/L, respectively, in human serum. Cat BChE differed from human BChE in the steady-state turnover of butyrylthiocholine, having a 3-fold higher k(cat) and 2-fold higher K(m) and K(ss) values. Sequencing of the cat BCHE cDNA revealed 70 amino acid differences between cat and human BChE, three of which could account for these kinetic differences. These amino acids, which were located in the region of the active site, were Phe398Ile, Pro285Leu, and Ala277Leu (where the first amino acid was found in human and the second in cat). Sequencing genomic DNA for cat and human ACHE demonstrated that there were 33 amino acid differences between the cat and human AChE enzymes, but that there were no differences in the active site region. In addition, a polymorphism in intron 3 of the human ACHE gene was detected, as well as a silent polymorphism at Y116 of the cat ACHE gene.
