Gene_locus Report for: culqu-b0w3q7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Juvenile hormone esterase
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Protostomia: N E > Ecdysozoa: N E > Panarthropoda: N E > Arthropoda: N E > Mandibulata: N E > Pancrustacea: N E > Hexapoda: N E > Insecta: N E > Dicondylia: N E > Pterygota: N E > Neoptera: N E > Holometabola: N E > Diptera: N E > Nematocera: N E > Culicomorpha: N E > Culicoidea: N E > Culicidae: N E > Culicinae: N E > Culicini: N E > Culex [genus]: N E > Culex [subgenus]: N E > Culex pipiens complex: N E > Culex quinquefasciatus: N E
A85-EsteraseD-FGH :
culqu-b0x7u1 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) S-formylglutathione hydrolase .
ABHD11-Acetyl_transferase :
culqu-b0x0a6 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). Esterase ybfF .
ABHD12-PHARC :
culqu-b0wf88 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). Putative uncharacterized protein .
ABHD13-BEM46 :
culqu-b0wi47 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). Abhydrolase domain-containing protein 13 .
ABHD18 :
culqu-b0w4e1 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Putative uncharacterized protein .
abh_upf0017 :
culqu-b0w5m5 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Putative uncharacterized protein ,
culqu-b0wpz0 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Putative uncharacterized protein .
ACHE :
culqu-ACHE1 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Acetylcholinesterase 1 ,
culqu-ACHE2 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Acetylcholinesterase 2 .
Acidic_Lipase :
culqu-b0w003 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w004 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g4 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g5 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g6 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g8 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6g9 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6h1 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6h2 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6h3 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0w6h4 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0wdk6 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0we24 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase 1 ,
culqu-b0wnv4 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0wxv5 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase ,
culqu-b0x4a4 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lysosomal acid lipase ,
culqu-b0xc32 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lipase .
Carb_B_Arthropoda :
culqu-1estb Culex quinquefasciatus (Southern house mosquito) C. pipiens, C. pipiens pipiens (Northern house mosquito) (C. pungens) CqestBeta1 CqestB1 Esterase B1, esterase b2 esterase b8 esterase b10 esterase b11,
culqu-2estb Culex quinquefasciatus (Southern house mosquito) serine esterase B ,
culqu-3estb Culex quinquefasciatus (PelSS) mRNA for serine esterase B ,
culqu-b0vz13 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0vz17 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0vz20 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0vz21 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0w8a7 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0w8a8 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0w8a9 Culex quinquefasciatus (Southern house mosquito) Glutactin ,
culqu-b0wbt7 Culex quinquefasciatus (Southern house mosquito) Para-nitrobenzyl esterase ,
culqu-b0wbt8 Culex quinquefasciatus (Southern house mosquito) Glutactin ,
culqu-b0wdn5 Culex quinquefasciatus (Southern house mosquito) Esterase B1 ,
culqu-b0wev9 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0wih9 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase-6 ,
culqu-b0wla4 Culex quinquefasciatus (Southern house mosquito) Esterase B1 ,
culqu-b0wla5 Culex quinquefasciatus (Southern house mosquito) Para-nitrobenzyl esterase ,
culqu-b0wla6 Culex quinquefasciatus (Southern house mosquito) Alpha-esterase ,
culqu-b0wla7 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase 2 ,
culqu-b0wla8 Culex quinquefasciatus (Southern house mosquito) Bile salt-activated lipase ,
culqu-b0wla9 Culex quinquefasciatus (Southern house mosquito) Alpha-esterase ,
culqu-b0wnx0 Culex quinquefasciatus (Southern house mosquito) Liver carboxylesterase 10 ,
culqu-b0wuu5 Culex quinquefasciatus (Southern house mosquito) Esterase E4 ,
culqu-b0x2a0 Culex quinquefasciatus (Southern house mosquito) Alpha-esterase ,
culqu-b0x9k7 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0x9k8 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0x746 Culex quinquefasciatus (Southern house mosquito) Esterase B1 ,
culqu-b0xam9 Culex quinquefasciatus (Southern house mosquito) Esterase B1 ,
culqu-b0xan0 Culex quinquefasciatus (Southern house mosquito) Esterase-5A ,
culqu-b0xan2 Culex quinquefasciatus (Southern house mosquito) Liver carboxylesterase 1 ,
culqu-b0xan3 Culex quinquefasciatus (Southern house mosquito) Esterase 6 ,
culqu-b0xan4 Culex quinquefasciatus (Southern house mosquito) Alpha-esterase ,
culqu-b0xaz2 Culex quinquefasciatus (Southern house mosquito) Esterase FE4 ,
culqu-b0xaz4 Culex quinquefasciatus (Southern house mosquito) esterase ,
culqu-b0xaz6 Culex quinquefasciatus (Southern house mosquito) Esterase FE4 ,
culqu-b0xaz7 Culex quinquefasciatus (Southern house mosquito) Esterase FE4 ,
culqu-b0xek9 Culex quinquefasciatus (Southern house mosquito) Juvenile hormone esterase ,
culqu-b0xfi1 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0xfi2 Culex quinquefasciatus (Southern house mosquito) esterase ,
culqu-b0xfi3 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase ,
culqu-b0xj03 Culex quinquefasciatus (Southern house mosquito) esterase .
CGI-58_ABHD5_ABHD4 :
culqu-b0wpg8 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Abhydrolase domain-containing protein 4 .
DPP4N_Peptidase_S9 :
culqu-b0we34 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Dipeptidyl peptidase 4 ,
culqu-b0wxf5 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Dipeptidyl-peptidase ,
culqu-b0wyn8 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Dipeptidyl-peptidase ,
culqu-b0wyp7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Dipeptidyl-peptidase .
Epoxide_hydrolase :
culqu-b0wft3 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Abhydrolase domain-containing protein 9 ,
culqu-b0wiu7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Epoxide hydrolase ,
culqu-b0xj28 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Abhydrolase domain-containing protein 7 ,
culqu-b0xj30 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Abhydrolase domain-containing protein 9 .
Gliotactin :
culqu-b0w0b8 Culex quinquefasciatus (Southern house mosquito) Gliotactin .
Insect_lipase :
culqu-q6try9 Culex quinquefasciatus (Southern house mosquito) putative salivary lipase (EC 3.1.1.3) .
Juvenile_hormone_esterase :
culqu-b0w3q8 Culex quinquefasciatus (Southern house mosquito) Gut esterase 1 ,
culqu-b0w3q9 Culex quinquefasciatus (Southern house mosquito) Lipase 4 ,
culqu-b0x0i0 Culex quinquefasciatus (Southern house mosquito) carboxylesterase 1 ,
culqu-b0x0i1 Culex quinquefasciatus (Southern house mosquito) Gut esterase 1 ,
culqu-b0x0i2 Culex quinquefasciatus (Southern house mosquito) Esterase 6 ,
culqu-b0x0i3 Culex quinquefasciatus (Southern house mosquito) Esterase FE4 ,
culqu-b0x4q0 Culex quinquefasciatus (Southern house mosquito) Esterase FE4 ,
culqu-b0x225 Culex quinquefasciatus (Southern house mosquito) Juvenile hormone esterase ,
culqu-b0xkv1 Culex quinquefasciatus (Southern house mosquito) Juvenile hormone esterase .
Kynurenine-formamidase :
culqu-b0w291 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). N-formylkynurenine formamidase .
LIDHydrolase :
culqu-b0wiv0 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Putative uncharacterized protein .
Ndr_family :
culqu-b0w346 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) N-myc downstream regulated .
Neurotactin :
culqu-b0wnn8 Culex quinquefasciatus (Southern house mosquito) Neurotactin .
NLS3-Tex30 :
culqu-b0wkn8 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Testis development protein prtd .
OtherNon-catalytic_C :
culqu-b0wji4 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase 3 ,
culqu-b0wri2 Culex quinquefasciatus (Southern house mosquito) Carboxylesterase .
Pancreatic_lipase :
culqu-b0vzr7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Culex pipiens pallens. Pancreatic triacylglycerol lipase precursor ,
culqu-b0xik3 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). Pancreatic triacylglycerol lipase precursor .
Pectinacetylesterase-Notum :
culqu-b0wi51 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Notum .
PPase_methylesterase_euk :
culqu-b0wub2 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Protein phosphatase methylesterase 1 .
Prolylcarboxypeptidase :
culqu-b0wgs7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0wgs8 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0wjz7 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Thymus-specific serine protease ,
culqu-b0wp51 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0wp52 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0wp54 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lysosomal pro-X carboxypeptidase ,
culqu-b0wp55 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0wp56 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Prolylcarboxypeptidase ,
culqu-b0x1w0 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Lysosomal Pro-X carboxypeptidase ,
culqu-b0xf46 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Thymus-specific serine protease ,
culqu-b0xf47 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Thymus-specific serine protease .
SERHL :
culqu-b0w294 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Valacyclovir hydrolase ,
culqu-b0w295 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Valacyclovir hydrolase ,
culqu-b0w232 Culex quinquefasciatus (Southern house mosquito) (Culex pungens). Valacyclovir hydrolase .
Valacyclovir-hydrolase :
culqu-b0w3i6 Culex quinquefasciatus (Southern house mosquito) (Culex pungens) Bphl protein Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can
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(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Culex quinquefasciatus quinquefasciatus:
N ,
E .
Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: culqu-b0w3q7 Colored MSA for Juvenile_hormone_esterase (raw)
MCELKKMLVLFILLCHVFAHETQRLGTEENTLQNPRVCIDDGCLRGKTME
GYQAGPFNAFIGIPYAKPPTEELRFSNPVRNEPWKRGIVYNATEDKPMCV
QKNDLLPNAKVSGDEDCLYLNVYQPMVHSSSKTPLPVMVYIHGGGFFAGG
ASPSIIGPEYFMDTRRVILVTFQYRLGVFGFLSTGDEVAPGNFGLKDQVM
ALRWVKHNIAYFGGNPDLVTIFGQSAGGASVHMHMISPMSDGLFSRSIVM
SGNAIAPWNIPTEDPLSLAQRQAEAVGITQVDTLSSKQLVDALRNVDANV
LSGSIDKLKFWSIDPLTLYRPVVEPLCSSNESFLIEDPRISWRKGSYQKI
PWMTGYLPNDGAVRAIAITSNEKLLNELNANISYILPMLLEKPSSQELMK
VLKLRYFNDSTDEKWITTENEQRLVDLYTEAAFLYPIQSAVKQHVTSADT
KLAPVSIYKFSFKGPYSYSFLYTFTHQDFGVVHCDELIYLFRSPALFPDF
PHKSKEARMSHHFVEFFIDFAINGVATPLKPYRGCNNDNEVYQSMNCDVL
EFINSSEPGKPFEVSKLNLNLPRFKVRKV
Legend
This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
M C E L K K M L V L F I L L C H V F A H E T Q R L G T E E N T L Q N P R V C I D D G C L R G K T M E G Y Q A G P F N A F I G I P Y A K P P T E E L R F S N P V R N E P W K R G I V Y N A T E D K P M C V Q K N D L L P N A K V S G D E D C L Y L N V Y Q P M V H S S S K T P L P V M V Y I H G G G F F A G G A S P S I I G P E Y F M D T R R V I L V T F Q Y R L G V F G F L S T G D E V A P G N F G L K D Q V M A L R W V K H N I A Y F G G N P D L V T I F G Q S A G G A S V H M H M I S P M S D G L F S R S I V M S G N A I A P W N I P T E D P L S L A Q R Q A E A V G I T Q V D T L S S K Q L V D A L R N V D A N V L S G S I D K L K F W S I D P L T L Y R P V V E P L C S S N E S F L I E D P R I S W R K G S Y Q K I P W M T G Y L P N D G A V R A I A I T S N E K L L N E L N A N I S Y I L P M L L E K P S S Q E L M K V L K L R Y F N D S T D E K W I T T E N E Q R L V D L Y T E A A F L Y P I Q S A V K Q H V T S A D T K L A P V S I Y K F S F K G P Y S Y S F L Y T F T H Q D F G V V H C D E L I Y L F R S P A L F P D F P H K S K E A R M S H H F V E F F I D F A I N G V A T P L K P Y R G C N N D N E V Y Q S M N C D V L E F I N S S E P G K P F E V S K L N L N L P R F K V R K V
Reference
Title: Juvenile hormone (JH) esterase of the mosquito Culex quinquefasciatus is not a target of the JH analog insecticide methoprene
Kamita SG , Samra AI , Liu JY , Cornel AJ , Hammock BD
Ref: PLoS ONE, 6 :e28392, 2011 : PubMed Abstract ESTHER: Kamita_2011_PLoS.ONE_6_E28392 PubMedSearch: Kamita 2011 PLoS.ONE 6 E28392 PubMedID: 22174797 Gene_locus related to this paper: culqu-b0w3q7 Inhibitor(s) related to this paper: Fenoxycarb Abstract
Juvenile hormones (JHs) are essential sesquiterpenes that control insect development and reproduction. JH analog (JHA) insecticides such as methoprene are compounds that mimic the structure and/or biological activity of JH. In this study we obtained a full-length cDNA, cqjhe, from the southern house mosquito Culex quinquefasciatus that encodes CqJHE, an esterase that selectively metabolizes JH. Unlike other recombinant esterases that have been identified from dipteran insects, CqJHE hydrolyzed JH with specificity constant (k(cat)/K(M) ratio) and V(max) values that are common among JH esterases (JHEs). CqJHE showed picomolar sensitivity to OTFP, a JHE-selective inhibitor, but more than 1000-fold lower sensitivity to DFP, a general esterase inhibitor. To our surprise, CqJHE did not metabolize the isopropyl ester of methoprene even when 25 pmol of methoprene was incubated with an amount of CqJHE that was sufficient to hydrolyze 7,200 pmol of JH to JH acid under the same assay conditions. In competition assays in which both JH and methoprene were available to CqJHE, methoprene did not show any inhibitory effects on the JH hydrolysis rate even when methoprene was present in the assay at a 10-fold higher concentration relative to JH. Our findings indicated that JHE is not a molecular target of methoprene. Our findings also do not support the hypothesis that methoprene functions in part by inhibiting the action of JHE.
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