(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Sauropsida: NE > Sauria: NE > Lepidosauria: NE > Squamata: NE > Bifurcata: NE > Unidentata: NE > Episquamata: NE > Toxicofera: NE > Serpentes: NE > Colubroidea: NE > Viperidae: NE > Crotalinae: NE > Crotalus: NE > Crotalus adamanteus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRKRNETVTLEHERFASSAPAPAAASSSSSPSLNKDFSLRPSPHLSGSAR PSGDSAMKRALSRRHGMWVRLRKVVTWLLGIYIAIPFLVKICPAIQAKLV FLNFVRVPYFIDLKRPQDQGLNHTSNFYLQPEEDVTIGVWHTVPTFLWKD AQGKDQLWYEDILGSNNPVILYLHGNAGTRGGDHRVELYKVLSFLGYHVV TFDYRGWGDSIGTPSESGMTYDALHVFDWIKARSGDNPVYIWGHSLGTGV ATNLVRRLCERETPPDALVLESPFTNIREEAKSHPFSVIYRYFPGFDWFF LDPITSSGIKFANDENVKHISCSLLILHAEDDPVVPFHLGKKLYNIAAPS RNFRDYKVQFVPFHKELGYRHKYIYKSPELPRILREFLGKSAHHHHQHH
Reference
Title: The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus) Rokyta DR, Lemmon AR, Margres MJ, Aronow K Ref: BMC Genomics, 13:312, 2012 : PubMed
BACKGROUND: Snake venoms have significant impacts on human populations through the morbidity and mortality associated with snakebites and as sources of drugs, drug leads, and physiological research tools. Genes expressed by venom-gland tissue, including those encoding toxic proteins, have therefore been sequenced but only with relatively sparse coverage resulting from the low-throughput sequencing approaches available. High-throughput approaches based on 454 pyrosequencing have recently been applied to the study of snake venoms to give the most complete characterizations to date of the genes expressed in active venom glands, but such approaches are costly and still provide a far-from-complete characterization of the genes expressed during venom production. RESULTS: We describe the de novo assembly and analysis of the venom-gland transcriptome of an eastern diamondback rattlesnake (Crotalus adamanteus) based on 95,643,958 pairs of quality-filtered, 100-base-pair Illumina reads. We identified 123 unique, full-length toxin-coding sequences, which cluster into 78 groups with less than 1% nucleotide divergence, and 2,879 unique, full-length nontoxin coding sequences. The toxin sequences accounted for 35.4% of the total reads, and the nontoxin sequences for an additional 27.5%. The most highly expressed toxin was a small myotoxin related to crotamine, which accounted for 5.9% of the total reads. Snake-venom metalloproteinases accounted for the highest percentage of reads mapping to a toxin class (24.4%), followed by C-type lectins (22.2%) and serine proteinases (20.0%). The most diverse toxin classes were the C-type lectins (21 clusters), the snake-venom metalloproteinases (16 clusters), and the serine proteinases (14 clusters). The high-abundance nontoxin transcripts were predominantly those involved in protein folding and translation, consistent with the protein-secretory function of the tissue. CONCLUSIONS: We have provided the most complete characterization of the genes expressed in an active snake venom gland to date, producing insights into snakebite pathology and guidance for snakebite treatment for the largest rattlesnake species and arguably the most dangerous snake native to the United States of America, C. adamanteus. We have more than doubled the number of sequenced toxins for this species and created extensive genomic resources for snakes based entirely on de novo assembly of Illumina sequence data.
