(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Deuterostomia: NE > Chordata: NE > Craniata: NE > Vertebrata: NE > Gnathostomata: NE > Teleostomi: NE > Euteleostomi: NE > Sarcopterygii: NE > Dipnotetrapodomorpha: NE > Tetrapoda: NE > Amniota: NE > Sauropsida: NE > Sauria: NE > Archelosauria: NE > Archosauria: NE > Dinosauria: NE > Saurischia: NE > Theropoda: NE > Coelurosauria: NE > Aves: NE > Neognathae: NE > Galloanserae: NE > Galliformes: NE > Phasianidae: NE > Phasianinae: NE > Gallus: NE > Gallus gallus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MASLWVRARRVFMKSRASGFSAKAATEMGSGGAEKGYRIPAGKGPHAVGC TDLMTGDAAEGSFLRLYYLSCDDTDTEETPWIPDKEYYQGLSDFLNVYRA LGERLFQYYVGSVTCPAKSNAAFKPGEKYPLLVFSHGLGAFRTIYSAICI EMASQGFLVAAVEHRDESASATYFCKKKADSEPEEDQTSGVEKEWIYYRK LRAGEEERCLRHKQVQQRAQECIKALNLILKISSGEEVMNVLNSDFDWNH LKDSVDTSRIAVMGHSFGGATVIESLSKEIRFRCGIALDAWMLPVGDDTY QSSVQQPLLFINSEKFQWAANILKMKKLSSNDTNKKMITIKGSVHQSFPD FTFVSGEIIGKFFKLKGEIDPNEAIDICNHASLAFLQKHLSLKRDFDKWD SLVDGIGPNVISGTNIDLSPTE
References
Title: Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad Tjoelker LW, Eberhardt C, Unger J, Le Trong H, Zimmerman GA Ref: Journal of Biological Chemistry, 270:25481, 1995 : PubMed
Platelet-activating factor (PAF) is a potent pro-inflammatory phospholipid that activates cells involved in inflammation. The biological activity of PAF depends on its structural features, namely an ether linkage at the sn-1 position and an acetate group at the sn-2 position. The actions of PAF are abolished by hydrolysis of the acetyl residue, a reaction catalysed by PAF acetylhydrolase. There are at least two forms of this enzyme--one intracellular and another that circulates in plasma and is likely to regulate inflammation. Here we report the molecular cloning and characterization of the human plasma PAF acetylhydrolase. The unique sequence contains a Gly-Xaa-Ser-Xaa-Gly motif commonly found in lipases. Recombinant PAF acetylhydrolase has the substrate specificity and lipoprotein association of the native enzyme, and blocks inflammation in vivo: it markedly decreases vascular leakage in pleurisy and paw oedema, suggesting that PAF acetylhydrolase might be a useful therapy for severe acute inflammation.
