(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Holometabola: NE > Diptera: NE > Brachycera: NE > Muscomorpha: NE > Eremoneura: NE > Cyclorrhapha: NE > Schizophora: NE > Acalyptratae: NE > Tephritoidea: NE > Tephritidae: NE > Dacinae: NE > Ceratitidini: NE > Ceratitis [genus]: NE > Ceratitis [subgenus]: NE > Ceratitis capitata: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MARTLALQAPSSLSASSRQHSFASSTSTLRLSSGDIGRGLFAIVILLLRM SSVYGVIDRLVVQTSSGPVRGRSVTVQGREVHVYTGIPYAKPPLDDLRFR KPVPAEPWHGVLDATRLPATCVQERYEYFPGFSGEEIWNPNTNVSEDCLY INVWAPAKARLRHGRGANGGEHSNKADTDHLIHNGNPQNTTNGLPVLIWI YGGGFMTGTATLDIYNADIMSAVGNVIVASFQYRVGAFGFLHLSPAMPGY EEEAPGNVGLWDQALAIRWLKTNAHAFGGNPEWMTLFGESAGSSSVNAQL VSPVTAGLVKRGMMQSGTMNAPWSHMTSEKAVEIGKALINDCNCNASLLA ENPQAVMACMRAVDAKTISVQQWNSYSGILSFPSAPTIDGAFLPDDPMKM METADMRGYDILMGNVRDEGTYFLLYDFIDYFDKDEATSLPRDKYLEIMN NIFGKVTQAEREAIIFRHTSWVGNPGLENQQQIGRAVGDHFFTCPTNEYA QVLAERGASVHYYYFTHRTSTSLWGEWMGVLHGDEIEYFFGQPLNTSLQY RQVERELGKRMLNAVIEFAKTGNPATDGEEWPNFTKKDPVYYVFSTDDKD EKLQRGPLEGRCAFWNEYLREVRKWGSQCDVKQSSASTIQQNMLQQKIIM ALLLALSIVLGIPSINAFF
Target site insensitivity and metabolic resistance mediated by esterases have been previously suggested to be involved in resistance to malathion in a field-derived strain (W) of Ceratitis capitata. In the present study, we have obtained the coding sequence for acetylcholinesterase (AChE) gene (Ccace) of C. capitata. An allele of Ccace carrying only a point mutation Gly328Ala (Torpedo numbering) adjacent to the glutamate of the catalytic triad was found in individuals of the W strain. Adult flies homozygotes for this mutant allele showed reduced AChE activity and less sensitivity to inhibition by malaoxon, showing that target site insensitivity is one of the factors of malathion resistance. In addition, all individuals from the resistant W strain showed reduced aliesterase activity, which has been associated with specific malathion resistance in higher Diptera. However, the alphaE7 gene (CcalphaE7), sequenced in susceptible and resistant individuals, did not carry any of the mutations associated with organophosphorus insecticide resistance in other Diptera. Another esterase mechanism, perhaps a carboxylesterase selective for malathion, in addition to mutant AChE, thus contributes to malathion resistance in C. capitata.
