Gene_locus Report for: brafl-ACHE2 Branchiostoma floridae (Florida lancelet) (Amphioxus) cholinesterase 2 (ChE2)
Comment
genes 119542 brafl-ACHEB (70800) brafl-ACHEA (116869) brafl-ACHE2 in a same contig scaffold_30 Another scafold_628 probably allelic to the other contains brafl-ACHE2 (273173) brafl-ACHEA (288582) brafl-ACHEB (132079) Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Deuterostomia: N E > Chordata: N E > Cephalochordata: N E > Branchiostomidae: N E > Branchiostoma: N E > Branchiostoma floridae: N E A85-EsteraseD-FGH :
brafl-c3yk61 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
ABHD6-Lip :
brafl-c3zci7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zlg0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zlg2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zlg3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
ABHD10 :
brafl-c3zj72 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein .
ABHD11-Acetyl_transferase :
brafl-c3z474 Branchiostoma floridae (Florida lancelet) (Amphioxus). AB hydrolase-1 domain-containing protein .
ABHD12-PHARC :
brafl-c3xr79 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein .
ABHD13-BEM46 :
brafl-c3zqr8 Branchiostoma floridae (Florida lancelet) (Amphioxus). AB hydrolase-1 domain-containing protein .
ABHD18 :
brafl-c3xyt7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
abh_upf0017 :
brafl-c3xvw5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y1p9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ymu0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
ACHE :
brafl-ACHE1 Branchiostoma floridae (Florida lancelet) (Amphioxus) cholinesterase 1 (ChE1) ,
brafl-ACHEA Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-ACHEB Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yne9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Acidic_Lipase :
brafl-c3xzy1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein Dhrs7C ,
brafl-c3xzy2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Lipase ,
brafl-c3zxg2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zxq3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Lipase .
Arylacetamide_deacetylase :
brafl-c3y7r2 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein ,
brafl-c3yj62 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein ,
brafl-c3zg22 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein .
BD-FAE :
brafl-c3z5c8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Carb_B_Chordata :
brafl-c3xqm2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx27 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx28 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx30 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx32 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx36 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx38 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx39 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx40 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xx41 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y7d7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y7s1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y8v8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y8w1.1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein probably pseudogene ,
brafl-c3y8w2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein incomplete ,
brafl-c3yan2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yc67 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yfm5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygc7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygc9.1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygd0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygd1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygd2.1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ygd4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ys59 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yv27 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z1u9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z1v0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z139 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zbr4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ziv7.1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ziv7.2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3znl2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zqg7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zqz2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
CGI-58_ABHD5_ABHD4 :
brafl-c3yi63 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yi64 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yi67 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yi68 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yi69 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yla7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Chlorophyllase_Plant :
brafl-c3xyd7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xyd8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xyd9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xye0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Cholinesterase-like :
brafl-c3xut6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xut7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y4l1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y6v9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y6y4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y8k5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein old b6lf84 b6lf83 b6lf82 b6lf81 new c3y8k5 c3y8k3 ,
brafl-c3y8t3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y8t4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y8t5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yfz8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yxb9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z3n7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z066 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z975 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zab8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zab9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zd14 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zer1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zmv1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zmv2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3znd6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zv54 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zvv1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
CMBL :
brafl-c3yde6 Branchiostoma floridae (Florida lancelet) (Amphioxus). DLH domain-containing protein .
DPP4N_Peptidase_S9 :
brafl-c3ydm8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yyq5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yz04 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Epoxide_hydrolase :
brafl-c3y9y3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z1c7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Haloalkane_dehalogenase-HLD2 :
brafl-c3xxt9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
LIDHydrolase :
brafl-c3yr72 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Maspardin-ACP33-SPG21_like :
brafl-c3y1t3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
MEST-like :
brafl-c3ylp9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ylq0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ylq1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Monoglyceridelipase_lysophospholip :
brafl-c3zfs6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zfs5 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein ,
brafl-c3zfs3 Branchiostoma floridae (Florida lancelet) (Amphioxus). Putative uncharacterized protein .
Ndr_family :
brafl-c3yim2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Neuroligin :
brafl-c3xqm5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y9i7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y9i8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3y9l9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yaw4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ybw7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yx17 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yx19 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3yxi7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3z9f4 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zf44 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zf47 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zf48 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zli5 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zs46 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zs49 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zs56 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zwz6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
NLS3-Tex30 :
brafl-c3ygr1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Pancreatic_lipase :
brafl-c3y2t9 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3y2u0 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3ycg1 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3ycg2 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3ycg4 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3z1l3 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein ,
brafl-c3zn71 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein .
Pectinacetylesterase-Notum :
brafl-c3xtl0 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3xtl1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ykb2 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ywf1 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zhm6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zme7 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zme8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
PhoPQ_related :
brafl-c3yra8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zmp8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
PMH_Peptidase_S9 :
brafl-c3y087 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
Prolylcarboxypeptidase :
brafl-c3y2u3 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ypm6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3ywh9 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein ,
brafl-c3zcy8 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein .
SERHL :
brafl-c3yf35 Branchiostoma floridae (Florida lancelet) (Amphioxus). Uncharacterized protein .
Valacyclovir-hydrolase :
brafl-c3ygg6 Branchiostoma floridae (Florida lancelet) (Amphioxus) Putative uncharacterized protein Molecular evidence
Database
8 mutations :
Table (e.g. : C310A/C466A , C310A/F312I , C310A ... more ) (less ) C310A/C466A : Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme
C310A/F312I : Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme
C310A : Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme
C466A : Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: the roles of two cysteines in the catalytic gorge of the enzyme
F312I : Amino acids defining the acyl pocket of an invertebrate cholinesterase
F422L : Amino acids defining the acyl pocket of an invertebrate cholinesterase
F422V : Amino acids defining the acyl pocket of an invertebrate cholinesterase
Y352A : Amino acids defining the acyl pocket of an invertebrate cholinesterase
No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: brafl-ACHE2 Colored MSA for ACHE (raw)
MAAMREYWFFVLLVNLTAHTWTAEAQTTNGPIVTTLQGRLQGKVLDVGGR
TVNAFLGIPYGAPPVGPRRFKPPIAAEPWNGIYNASSYPNTCMQLPDTTF
PGYKGAEMWNPNTPVSEDCLYLNVWQPSPVPVGATVMVWIYGGGFMSGTS
SLDVYDGRYLARTQGVIVASMNYRTGAMGFLSLGNSEAPGNVGLMDQNLA
LTWIKENVASFGGAPNKVSIFGESAGAASVSYHLLSPMSKNLFQRAIMES
ASAPSPWALLSDTEAYRRGIELPKAVGCSTDSDLEETIECMRGVPAQTIS
DNEWVVWGLCQFPFAPIVDGNFIREHPTQSLQTGNLKQTDVLVGFNNDEG
VYFLLYGAPGFSKDTRSLITREQYLEGIKMSVMGINDISVDALSFQYIDW
VNFDQPSMYRDAIDDLSGDYNFICPALSFGKAMASLGRKTYQYKFVHQAS
NMPWPKWTGVMHGYECEFVFGLPTDQKNNYTQEEAVFATQIMTYWANFAK
TGNPNKQTLEDPAPDVWRPYTDEGQEYLILDVGGNRMANGPRSKSCAFWD
NYLWELDRKTDDLMEQAQSCTNGAEIPRASTCTAIFSTAILLLLLNSATT
LL
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M A A M R E Y W F F V L L V N L T A H T W T A E A Q T T N G P I V T T L Q G R L Q G K V L D V G G R T V N A F L G I P Y G A P P V G P R R F K P P I A A E P W N G I Y N A S S Y P N T C M Q L P D T T F P G Y K G A E M W N P N T P V S E D C L Y L N V W Q P S P V P V G A T V M V W I Y G G G F M S G T S S L D V Y D G R Y L A R T Q G V I V A S M N Y R T G A M G F L S L G N S E A P G N V G L M D Q N L A L T W I K E N V A S F G G A P N K V S I F G E S A G A A S V S Y H L L S P M S K N L F Q R A I M E S A S A P S P W A L L S D T E A Y R R G I E L P K A V G C S T D S D L E E T I E C M R G V P A Q T I S D N E W V V W G L C Q F P F A P I V D G N F I R E H P T Q S L Q T G N L K Q T D V L V G F N N D E G V Y F L L Y G A P G F S K D T R S L I T R E Q Y L E G I K M S V M G I N D I S V D A L S F Q Y I D W V N F D Q P S M Y R D A I D D L S G D Y N F I C P A L S F G K A M A S L G R K T Y Q Y K F V H Q A S N M P W P K W T G V M H G Y E C E F V F G L P T D Q K N N Y T Q E E A V F A T Q I M T Y W A N F A K T G N P N K Q T L E D P A P D V W R P Y T D E G Q E Y L I L D V G G N R M A N G P R S K S C A F W D N Y L W E L D R K T D D L M E Q A Q S C T N G A E I P R A S T C T A I F S T A I L L L L L N S A T T L L
References
Title: The amphioxus genome and the evolution of the chordate karyotypePutnam NH , Butts T , Ferrier DE , Furlong RF , Hellsten U , Kawashima T , Robinson-Rechavi M , Shoguchi E , Terry A and Rokhsar DS <27 more author(s)> Putnam NH , Butts T , Ferrier DE , Furlong RF , Hellsten U , Kawashima T , Robinson-Rechavi M , Shoguchi E , Terry A , Yu JK , Benito-Gutierrez EL , Dubchak I , Garcia-Fernandez J , Gibson-Brown JJ , Grigoriev IV , Horton AC , de Jong PJ , Jurka J , Kapitonov VV , Kohara Y , Kuroki Y , Lindquist E , Lucas S , Osoegawa K , Pennacchio LA , Salamov AA , Satou Y , Sauka-Spengler T , Schmutz J , Shin IT , Toyoda A , Bronner-Fraser M , Fujiyama A , Holland LZ , Holland PW , Satoh N , Rokhsar DS (- 27) Ref: Nature, 453 :1064, 2008 : PubMed Abstract ESTHER: Putnam_2008_Nature_453_1064 PubMedSearch: Putnam 2008 Nature 453 1064 PubMedID: 18563158 Gene_locus related to this paper: brafl-ACHE1 ,
brafl-ACHE2 ,
brafl-ACHEA ,
brafl-ACHEB ,
brafl-c3xqm2 ,
brafl-c3xqm5 ,
brafl-c3xtl0 ,
brafl-c3xtl1 ,
brafl-c3xut6 ,
brafl-c3xut7 ,
brafl-c3xvw5 ,
brafl-c3xx27 ,
brafl-c3xx28 ,
brafl-c3xx30 ,
brafl-c3xx32 ,
brafl-c3xx36 ,
brafl-c3xx38 ,
brafl-c3xx39 ,
brafl-c3xx40 ,
brafl-c3xx41 ,
brafl-c3xxt9 ,
brafl-c3xyd7 ,
brafl-c3xyd8 ,
brafl-c3xyd9 ,
brafl-c3xye0 ,
brafl-c3xyt7 ,
brafl-c3xzy1 ,
brafl-c3xzy2 ,
brafl-c3y1p9 ,
brafl-c3y1t3 ,
brafl-c3y2u3 ,
brafl-c3y4l1 ,
brafl-c3y6v9 ,
brafl-c3y6y4 ,
brafl-c3y7d7 ,
brafl-c3y7s1 ,
brafl-c3y8k5 ,
brafl-c3y8t3 ,
brafl-c3y8t4 ,
brafl-c3y8t5 ,
brafl-c3y8v8 ,
brafl-c3y8w1.1 ,
brafl-c3y8w2 ,
brafl-c3y9i7 ,
brafl-c3y9i8 ,
brafl-c3y9l9 ,
brafl-c3y9y3 ,
brafl-c3y087 ,
brafl-c3yan2 ,
brafl-c3yaw4 ,
brafl-c3ybw7 ,
brafl-c3yc67 ,
brafl-c3ydm8 ,
brafl-c3yfm5 ,
brafl-c3yfz8 ,
brafl-c3ygc7 ,
brafl-c3ygc9.1 ,
brafl-c3ygd0 ,
brafl-c3ygd1 ,
brafl-c3ygd2.1 ,
brafl-c3ygd4 ,
brafl-c3ygg6 ,
brafl-c3ygr1 ,
brafl-c3yi63 ,
brafl-c3yi64 ,
brafl-c3yi67 ,
brafl-c3yi68 ,
brafl-c3yi69 ,
brafl-c3yk61 ,
brafl-c3ykb2 ,
brafl-c3yla7 ,
brafl-c3ylp9 ,
brafl-c3ylq0 ,
brafl-c3ylq1 ,
brafl-c3ymu0 ,
brafl-c3yne9 ,
brafl-c3ypm6 ,
brafl-c3yr72 ,
brafl-c3yra8 ,
brafl-c3ys59 ,
brafl-c3yv27 ,
brafl-c3ywf1 ,
brafl-c3ywh9 ,
brafl-c3yx17 ,
brafl-c3yx19 ,
brafl-c3yxb9 ,
brafl-c3yxi7 ,
brafl-c3yyq5 ,
brafl-c3yz04 ,
brafl-c3z1c7 ,
brafl-c3z1u9 ,
brafl-c3z1v0 ,
brafl-c3z3n7 ,
brafl-c3z5c8 ,
brafl-c3z9f4 ,
brafl-c3z066 ,
brafl-c3z139 ,
brafl-c3z975 ,
brafl-c3zab8 ,
brafl-c3zab9 ,
brafl-c3zbr4 ,
brafl-c3zci7 ,
brafl-c3zcy8 ,
brafl-c3zd14 ,
brafl-c3zer1 ,
brafl-c3zf44 ,
brafl-c3zf47 ,
brafl-c3zf48 ,
brafl-c3zfs6 ,
brafl-c3zhm6 ,
brafl-c3ziv7.1 ,
brafl-c3ziv7.2 ,
brafl-c3zlg0 ,
brafl-c3zlg2 ,
brafl-c3zlg3 ,
brafl-c3zli5 ,
brafl-c3zme7 ,
brafl-c3zme8 ,
brafl-c3zmp8 ,
brafl-c3zmv1 ,
brafl-c3zmv2 ,
brafl-c3znd6 ,
brafl-c3znl2 ,
brafl-c3zqg7 ,
brafl-c3zqz2 ,
brafl-c3zs46 ,
brafl-c3zs49 ,
brafl-c3zs56 ,
brafl-c3zv54 ,
brafl-c3zvv1 ,
brafl-c3zwz6 ,
brafl-c3zxg2 ,
brafl-c3zxq3 ,
brafl-c3yim2 ,
brafl-c3zfs5 ,
brafl-c3zfs3 ,
brafl-c3xr79 ,
brafl-c3y7r2 ,
brafl-c3yj62 ,
brafl-c3zg22 ,
brafl-c3y2t9 ,
brafl-c3y2u0 ,
brafl-c3ycg1 ,
brafl-c3ycg2 ,
brafl-c3ycg4 ,
brafl-c3z1l3 ,
brafl-c3zn71 ,
brafl-c3zj72 ,
brafl-c3yf35 ,
brafl-c3z474 ,
brafl-c3zqr8 ,
brafl-c3yde6 Abstract
Lancelets ('amphioxus') are the modern survivors of an ancient chordate lineage, with a fossil record dating back to the Cambrian period. Here we describe the structure and gene content of the highly polymorphic approximately 520-megabase genome of the Florida lancelet Branchiostoma floridae, and analyse it in the context of chordate evolution. Whole-genome comparisons illuminate the murky relationships among the three chordate groups (tunicates, lancelets and vertebrates), and allow not only reconstruction of the gene complement of the last common chordate ancestor but also partial reconstruction of its genomic organization, as well as a description of two genome-wide duplications and subsequent reorganizations in the vertebrate lineage. These genome-scale events shaped the vertebrate genome and provided additional genetic variation for exploitation during vertebrate evolution.
Title: cDNA cloning, in vitro expression, and biochemical characterization of cholinesterase 1 and cholinesterase 2 from amphioxus--comparison with cholinesterase 1 and cholinesterase 2 produced in vivoMcClellan JS , Coblentz WB , Sapp M , Rulewicz G , Gaines DI , Hawkins A , Ozment C , Bearden A , Merritt S and Pezzementi L <3 more author(s)> McClellan JS , Coblentz WB , Sapp M , Rulewicz G , Gaines DI , Hawkins A , Ozment C , Bearden A , Merritt S , Cunningham J , Palmer E , Contractor A , Pezzementi L (- 3) Ref: European Journal of Biochemistry, 258 :419, 1998 : PubMed Abstract ESTHER: McClellan_1998_Eur.J.Biochem_258_419 PubMedSearch: McClellan 1998 Eur.J.Biochem 258 419 PubMedID: 9874207 Gene_locus related to this paper: brafl-ACHE1 ,
brafl-ACHE2 Abstract
We have isolated cDNAs coding for the complete amino acid sequences of cholinesterase 1 (ChE1) and cholinesterase 2 (ChE2) from amphioxus. Both ChE transcripts have the characteristics of H-type catalytic subunits, which are inserted in the membrane via an ethanolamine-glycan-phosphatidylinositol anchor. The members of the catalytic triad of ChEs, the three pairs of cysteine residues involved in intrachain disulfide bonding, a cysteine near the carboxy terminal of both sequences, which could mediate interchain disulfide bonding, and 11 of the 14 aromatic amino acids that line the catalytic gorge of AChE are conserved. A remarkable difference between the two enzymes is in the region of the acyl-binding pocket, which plays an important role in determining substrate specificity in cholinesterases. ChE2 contains a sequence that resembles the acyl pocket of invertebrate ChE, while the acyl-binding site of ChE1 is novel. There are also differences between the two enzymes in the peripheral anionic site, which mediates inhibition by certain ligands. In vitro expression in COS-7 cells demonstrates that ChE2 hydrolyzes acetylthiocholine almost exclusively, while ChE1 hydrolyzes both acetylthiocholine and butyrylthiocholine. Both enzymes are inhibited comparably by BW284c51, but ChE1 is considerably more resistant to inhibition by propidium, ethopropazine, and eserine than is ChE2. Velocity sedimentation indicates that ChE1 and ChE2 are present as amphiphilic and nonamphiphilic G2 forms in vivo and in vitro. Another molecular form, which sediments at 17 S, is also present in vivo. Nondenaturing gel electrophoresis in conjunction with digestion by phosphatidylinositol-specific phospholipase C demonstrates that the vast majority of ChE1 and ChE2 is present as ethanolamine-glycan-phosphatidylinositol-anchored G2 forms in vivo. ChE1 also possesses an ethanolamine-glycan-phosphatidylinositol-anchor in vitro; however, ChE2 produced in vitro could not be detected on nondenaturing gels.
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