(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Polyneoptera: NE > Dictyoptera: NE > Blattodea: NE > Blaberoidea: NE > Ectobiidae: NE > Blattellinae: NE > Blattella: NE > Blattella germanica: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MATQCLLILAGCICTAMAIGAGSKPHFNSSSASSTPHSTIGGGSSPNTNP GKLDYHDDPLVVETQSGLVRGSARVVLGKEVHIFTGIPFAKPPVGPLRFR RPVPVEPWHGVLDATALPNSCYQERYEYFKGFEGEEMWNPNTNVSEDCLY LNIWVPQRVRLRHSGERPKVPILVWIYGGGYMSGTSTLDVYDADIVVATS DIIVASMQYRVGSFGFLYLKPFFGPDSEEAPGNMGMWDQALAIRWLKDNA AAFGGDPDLITLFGESAGGGSVSLHLMSPITKGLVRRGIMQSGTLNAPWS YMTGEKAADIGRVLVEDVGCNSTQLSEAPSKVMACLRSVDSKAISVKQWN SYWGILGFPSAPTIDGVFLPKHPMDLIKELDWEDTEILIGSNQDEGTYFI LYDFMDYFEKDSPTFLQRDKYLDIVNLIFKNMTRLERDAIIFQYTDWEHL ADGYKNQRMIADVVGDYFFICPTNVFAEQFAEHGTKVYYYYFTQRTSLNL WGQWMGVMHGDEIEYVFGHPLNMSIDYNDNERDLSLRIMEIYSRFALTGK PIANEADWPTYTKDHPKYYILNSDTYGTGKGPRTTNCAFWNDFLPKLANH PDPDYACASGVAETANLGVNSATSCLLVGPKHLLYFVTIVLARLLLSL
References
Title: Functional analysis and molecular characterization of two acetylcholinesterases from the German cockroach, Blattella germanica Kim YH, Choi JY, Je YH, Koh YH, Lee SH Ref: Insect Molecular Biology, 19:765, 2010 : PubMed
Two acetylcholinesterases (AChEs; BgAChE1 and BgAChE2) from Blattella germanica were functionally expressed using the baculovirus system. Kinetic analysis demonstrated that BgAChE2 had higher catalytic efficiency but lower substrate specificity than BgAChE1. With the exceptions of paraoxon and propoxur, BgAChE1 was generally less sensitive to inhibitors than BgAChE2. Western blot analysis using anti-BgAChE antibodies revealed that BgAChE1 was far more abundant in all examined tissues compared to BgAChE2, which is only present in the central nervous system. Both BgAChEs existed in dimeric form, covalently connected via a disulphide bridge under native conditions. Most fractions of BgAChE1 had a glycophosphatidylinositol (GPI) anchor, but a small fraction comprised a collagen-like tail. BgAChE2 appeared to have a collagen-GPI-fused tail. Based on the kinetic and molecular properties, tissue distribution and abundance, BgAChE1 was confirmed to play a major role in postsynaptic transmission.
Title: Molecular, biochemical and histochemical characterization of two acetylcholinesterase cDNAs from the German cockroach Blattella germanica Kim JI, Jung CS, Koh YH, Lee SH Ref: Insect Molecular Biology, 15:513, 2006 : PubMed
Full length cDNAs encoding two acetylcholinesterases (AChEs; Bgace1 and Bgace2) were cloned and characterized from the German cockroach, Blattella germanica. Sequence analyses showed that both genes possess all the typical features of ace, and that Bgace1 is orthologous to the insect ace1 whereas Bgace2 is to the insect ace2. Transcript level of Bgace1 was significantly higher (c. 10 fold) than that of Bgace2 in all 11 tissues examined, suggesting that Bgace1 likely encodes a predominant AChE. Multiple AChE bands were identified by native polyacrylamide gel electrophoresis and isoelectricfocusing from various tissue preparations, among which ganglia produced distinct two major and two minor AChE bands, indicative of the presence of at least two active AChEs. B. germanica AChEs appeared to be mainly localized in the central nervous system as demonstrated by histochemical activity staining, together with quantitative analysis of Bgace transcripts. Fluorescence in situ hybridization of the 1st thoracic ganglion confirmed that Bgace1 is predominantly transcribed and further showed that its transcript is found in almost entire region of inter or motor neurones including the cell bodies and axonal/dendritic branches. Bgace2 transcript is found only in the subset of neurones, particularly in the cell body. In addition, certain neurones were observed to express Bgace1 only.
