(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Bacilli: NE > Bacillales: NE > Bacillaceae: NE > Bacillus: NE > Bacillus pumilus: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Bacillus pumilus ATCC 7061: N, E.
Bacillus pumilus SAFR-032: N, E.
Bacillus subtilis BSn5: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MTHQIVTTQYGKVKGTTENGVHQWKGIPYAKPPVGQWRFKAPEPPEVWED VLDATAYGPVCPQPSDLLSLSYTELPRQSEDCLFVNVFAPDTPSQNLPVM VWIHGGAFYLGAGSEPLYDGSKLAAQGEVIVVTLNYRLGPFGFLHLSSFD EAYSDNLGLLDQVAALKWVRENISAFGGDPDNATVFGESAGGMSIAALLA MPAAKGLFQKAIMESGASRTMTKEQAASTSAAFLQVLGINEGQLDKLHTV SAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPAEPEKAIAEGAASGI PLLIGTTRDEGYLFFTPDSDVHSQETLDAALEYLLGKPLAEKAADLYPRS LESQIHMMTDLLFWRPAVAYASAHSHYAPVWMYRFDWHPEKPPYNKTFHA LELPFVFGNLDGLERMARAEVTDEAKQLSHSIQSAWITFAKTGNPSTEAV NWPAYHEETRETLILDSEITIENDPESEKRQKLFPSKGE
References
Title: Complete genome sequence of Bacillus subtilis BSn5, an endophytic bacterium of Amorphophallus konjac with antimicrobial activity for the plant pathogen Erwinia carotovora subsp. carotovora Deng Y, Zhu Y, Wang P, Zhu L, Zheng J, Li R, Ruan L, Peng D, Sun M Ref: Journal of Bacteriology, 193:2070, 2011 : PubMed
Here, we present the complete genome sequence of Bacillus subtilis strain BSn5, isolated from Amorphophallus konjac calli tissue and showing strong inhibitory activity to Erwinia carotovora subsp. carotovora, which causes Amorphophallus soft rot disease and affects the industry development of this organism.
Title: Development of a versatile cassette for directional genome walking using cassette ligation-mediated PCR and its application in the cloning of complete lipolytic genes from Bacillus species Nthangeni MB, Ramagoma F, Tlou MG, Litthauer D Ref: J Microbiol Methods, 61:225, 2005 : PubMed
Since the invention of the PCR technology, adaptation techniques to clone DNA fragments flanking the known sequence continue to be developed. We describe a perfectly annealed cassette available in almost unlimited quantities with variable sticky-and blunt-end restriction enzyme recognition sites for efficient restriction and ligation with the restricted target genomic DNA. The cassette provides a 200-bp sequence, which is used to design a variety of cassette-specific primers. The dephosphorylation prevents cassette self-ligation and creates a nick at the cassette: target genome DNA ligation site suppressing unspecific PCR amplifications. We introduce the single-strand amplification PCR (SSA-PCR) technique where a lone known locus-specific primer is firstly used to enrich the targeted template DNA strand resulting in significant PCR product specificity during the second round conventional nested PCR. The distance between the known locus-specific primer and the nearest location of the restriction enzyme used determined the length of the obtained PCR product. We used this technique to walk downstream into the isochorismatase and upstream into the hypothetical conserved genes flanking the mature extracellular lipase gene from Bacillus licheniformis. We further demonstrated the potential of the technique as a cost-effective method during PCR-based prospecting for novel genes by designing "universal" degenerate primers that detected homologues of Family VII bacterial lipolytic genes in Bacillus species. The cassette ligation-mediated PCR was used to clone complete nucleotide sequences encoding functional lipolytic genes from B. licheniformis and Bacillus pumilus.
Bacillus pumilus (Bacillus mesentericus) (strain SAFR-032; ATCC 7061) acetyl xylan esterase (EC 3.1.1.6)