Gene_locus Report for: ascsu-f1kpy0.3

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Metazoa: N E > Eumetazoa: N E > Bilateria: N E > Protostomia: N E > Ecdysozoa: N E > Nematoda: N E > Chromadorea: N E > Ascaridida: N E > Ascaridoidea: N E > Ascarididae: N E > Ascaris: N E > Ascaris suum: N E

Title: Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles
Wang J, Czech B, Crunk A, Wallace A, Mitreva M, Hannon GJ, Davis RE
Ref: Genome Res, 21:1462, 2011 : PubMed
Abstract


ESTHER: Wang_2011_Genome.Res_21_1462
PubMedSearch: Wang 2011 Genome.Res 21 1462
PubMedID: 21685128
Gene_locus related to this paper: ascsu-f1kpy0.2, ascsu-f1kpy0.3, ascsu-f1kpy0.4, ascsu-f1kr69, ascsu-f1ktc3, ascsu-f1ktc5, ascsu-f1ktw1.1, ascsu-f1ktw1.2, ascsu-f1ktx4, ascsu-f1kwb2, ascsu-f1kwb5, ascsu-f1kwf0, ascsu-f1kxs8, ascsu-f1ky57, ascsu-f1kz22, ascsu-f1kze8, ascsu-f1kzv8, ascsu-f1kzx8, ascsu-f1l0a5, ascsu-f1l0j3, ascsu-f1l0s4, ascsu-f1l0s5, ascsu-f1l1k4, ascsu-f1l1m9, ascsu-f1l1p8, ascsu-f1l2e5, ascsu-f1l2m0, ascsu-f1l3k2, ascsu-f1l5n5, ascsu-f1l6s4, ascsu-f1l7s2, ascsu-f1l108, ascsu-f1l145, ascsu-f1la00, ascsu-f1lav9, ascsu-u1mei8, ascsu-u1ns34, ascsu-u1nb30, ascsu-f1kzg5, ascsu-f1l726, ascsu-u1ntf1, ascsu-u1nx87, ascsu-f1l5f0, ascsu-f1l6n2

Abstract

Eukaryotic cells express several classes of small RNAs that regulate gene expression and ensure genome maintenance. Endogenous siRNAs (endo-siRNAs) and Piwi-interacting RNAs (piRNAs) mainly control gene and transposon expression in the germline, while microRNAs (miRNAs) generally function in post-transcriptional gene silencing in both somatic and germline cells. To provide an evolutionary and developmental perspective on small RNA pathways in nematodes, we identified and characterized known and novel small RNA classes through gametogenesis and embryo development in the parasitic nematode Ascaris suum and compared them with known small RNAs of Caenorhabditis elegans. piRNAs, Piwi-clade Argonautes, and other proteins associated with the piRNA pathway have been lost in Ascaris. miRNAs are synthesized immediately after fertilization in utero, before pronuclear fusion, and before the first cleavage of the zygote. This is the earliest expression of small RNAs ever described at a developmental stage long thought to be transcriptionally quiescent. A comparison of the two classes of Ascaris endo-siRNAs, 22G-RNAs and 26G-RNAs, to those in C. elegans, suggests great diversification and plasticity in the use of small RNA pathways during spermatogenesis in different nematodes. Our data reveal conserved characteristics of nematode small RNAs as well as features unique to Ascaris that illustrate significant flexibility in the use of small RNAs pathways, some of which are likely an adaptation to Ascaris' life cycle and parasitism. The transcriptome assembly has been submitted to NCBI Transcriptome Shotgun Assembly Sequence Database(http:\/\/www.ncbi.nlm.nih.gov/genbank/TSA.html) under accession numbers JI163767-JI182837 and JI210738-JI257410.