(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Pezizomycotina: NE > Pezizomycetes: NE > Pezizales: NE > Ascobolaceae: NE > Ascobolus: NE > Ascobolus immersus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MHLVDRVGANAPHKKYERVTTQPENPFFNIVHNQSVAIIPSFTLESGVIL YDCPVAYKTFGVLNESADNVMVICHALTGSADVEDWWGPLIGPGRAFDTS RYFIVCCNSMGSPYGSASPCTLDSTTGRRYGPEFPLTTVRDDVRYGSTIT MKLGCLLTYYRIHKLIMDDLGVRQIAVVIGGSMGGMLALEWAYFGKDYVK AVVALATSARHSAWCISWGEAQRQSIYSDPKYDDGYYSFSDPPYTGLGAA RMSALLTYRSRNSFESRFGRNIPDPSRHPYINTSQPPSHPAEEHYDIHNE GFRNRKGFRRSSTTTSDAPPSPTRTSSTSSTDAITPASTTPLHPSRAQPS GIATPPNSVSDPFRPVKRPCPTYFSAQSYLRYQADKFVKRFDANCYIAIT RKLDTHDVSRGRTSTLHEALAMIEQPTLIIGIESDGLFTFAEQMELAEYI PDARLKRIDSPEGHDAFLIMFAEVNRYICEFLREVQPEIMGKEGVHVESK VGEIRASTTGEVEDITHW
Reference
Title: Molecular cloning and characterization of the met2 gene from Ascobolus immersus Goyon C, Faugeron G, Rossignol JL Ref: Gene, 63:297, 1988 : PubMed
We have cloned the met2 gene from Ascobolus immersus by heterologous hybridization with the MET2 gene of Saccharomyces cerevisiae. This gene codes for the homoserine O-transacetylase, one of the methionine biosynthetic enzymes. The complete nucleotide sequence of a 2910-bp DNA fragment carrying the met2 gene has been determined. The gene contains a 165-bp intron which is similar in structure to other fungal introns. The deduced amino acid (aa) sequence (518 aa residues; Mr of 57726) shows three domains with a significant level of homology with the corresponding yeast protein. Northern-blot analysis reveals at least two transcripts (2.4 and 2.1 kb) probably due to transcription termination heterogeneity, as suggested by S1-mapping experiments. Polymorphism has been observed in the met2 gene flanking regions of Ascobolus strains from two different stocks.