9zzzz-AncHLDRLuc
Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase
Comment
Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>)
Relationship
Molecular evidence
Database
Sequence
Peptide
ATGDEWWAKC KQVDVLDSEM SYYDSDPGKH KNTVIFLHGN PTSSYLWRNV IPHVEPLARC LAPDLIGMGK SGKLPNHSYR FVDHYRYLSA WFDSVNLPEK VTIVCHDWGS GLGFHWCNEH RDRVKGIVHM ESVVSPLKGW ESFPETARDI FQALRSEAGE EMVLKKNFFI ERLLPSSIMR KLSEEEMDAY REPFVEPGES RRPTLTWPRE IPIKGDGPED VIEIVKSYNK WLSTSKDIPK LFINADPGFF SNAIKKVTKN WPNQKTVTVK GLHFLQEDSP EEIGEAIADF LNELTK
References
| Title : Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts - Chaloupkova_2019_ACS.Catal_9_48103 |
| Author(s) : Chaloupkova, R , Liskova V , Tool M , Markova K , Sebestova E , Hernychova L , Marek M , Pinto GP , Pluskal D , Waterman J , Prokop Z , Damborsky J |
| Ref : ACS Catal , 9 :4810 , 2019 |
| Abstract : Chaloupkova_2019_ACS.Catal_9_48103 |
| ESTHER : Chaloupkova_2019_ACS.Catal_9_48103 |
| PubMedSearch : Chaloupkova_2019_ACS.Catal_9_48103 |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , 9zzzz-AncHLDRLuc , renre-luc |