9zzzz-5TDX
Resurrected ancestral hydroxynitrile lyase from flowering plants
Comment
Authors show that resurrected ancestral plant esterases are as catalytically specific as modern esterases, that the ancestor of modern acetone cyanohydrin lyases was itself only very weakly promiscuous, and that improvements in lyase activity came at the expense of esterase activity
Relationship
Molecular evidence
Database
No Genbank
No Interpro
No Pfam
No UniProt
No PIRSF
No SUPERFAM
Sequence
Peptide
MATAHFVLIH TICHGAWIWY KLKPLLEAAG HKVTALDLAA SGIDPRQIEQ INTFDEYSEP LLTFMESLPQ GEKVILVGES CGGLNIALAA DKYPEKISAA VFHNALMPDT EHSPSYVVDK FMEVFPDWKD TEFSTYTSNN ETITGMKLGF KLMRENLYTN CPIEDYELAK MLTRKGSFFQ NDLAQRPKFT EEGYGSIKRV YVWTDEDKIF PPEFQLWQIE NYKPDKVYRV QGGDHKLQLS KTNELAEILQ EVADTYADLL AVAGLE
References
| Title : Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity - Jones_2020_PLoS.One_15_e0235341 |
| Author(s) : Jones BJ , Evans RL, 3rd , Mylrea NJ , Chaudhury D , Luo C , Guan B , Pierce CT , Gordon WR , Wilmot CM , Kazlauskas RJ |
| Ref : PLoS ONE , 15 :e0235341 , 2020 |
| Abstract : Jones_2020_PLoS.One_15_e0235341 |
| ESTHER : Jones_2020_PLoS.One_15_e0235341 |
| PubMedSearch : Jones_2020_PLoS.One_15_e0235341 |
| PubMedID: 32603354 |
| Gene_locus related to this paper: 9zzzz-5TDX |
| Title : Evolution of a Catalytic Mechanism - Rauwerdink_2016_Mol.Biol.Evol_33_971 |
| Author(s) : Rauwerdink A , Lunzer M , Devamani T , Jones B , Mooney J , Zhang ZJ , Xu JH , Kazlauskas RJ , Dean AM |
| Ref : Molecular Biology Evolution , 33 :971 , 2016 |
| Abstract : Rauwerdink_2016_Mol.Biol.Evol_33_971 |
| ESTHER : Rauwerdink_2016_Mol.Biol.Evol_33_971 |
| PubMedSearch : Rauwerdink_2016_Mol.Biol.Evol_33_971 |
| PubMedID: 26681154 |
| Gene_locus related to this paper: 9zzzz-5TDX , hevbr-hnl |