Malus pumila (Paradise apple); Malus domestica (Apple) (Pyrus malus) CXE1 carboxylesterase MdCXE1 (EC 3.1.1.1)
Other strains: Malus pumila (Paradise apple)\; Malus domestica (Apple) (Pyrus malus) || Marshall S.D.G., Oakeshott J.G., Russell R.J., Plummer K.M., Newcomb R.D || MdCXE1 is expressed at low levels during early stages of fruit development, rising to a peak of expression in apple fruit at harvest maturity. MdCXE1 hydrolyse 4-methyl umbelliferyl ester substrates that included those containing acyl moieties that are found in esters produced by apple fruit. MdCXE1 is inhibited by organophosphates. MdCXE1 showed increasing affinity (Km) but decreasing turnover (kcat) as substrate acyl carbon length increases from C2 to C16. MdCXE1 may have an impact on apple flavour through its ability to hydrolyse relevant flavour esters in ripe apple fruit.
MTTSLDSSTQ KTAPSWTSLP WRTRVSLSVL SKITDAARRA DGTVNRRLMN ILDFKSSATP AAPVRGVTSS DVTVDPARKL WFRLFVPQST LSTPSDLPVI VFFHGGGFTF LSPASFAYNA VCRKFARKFP AVVVSVNYRL CPEHRYPSPY DDGFDVLTFL DQNDDVLPKN ADRSRIFLAG DSAGANVAHH VAVRAAREKD RMRVVKPVGL ISIQPFFGGE ERVESEIRLR GAPLVSVGRT DWLWKVFLPD GSNRDHEAAN VSGPNAVDIS GLEYPNTIVF TGGLDPLLDR QRRYYQWLKK SGKEAKLIEY PNMVHAFYVF PELPESNQLI NQVKDFIASV
| Title : Biochemical characterisation of MdCXE1, a carboxylesterase from apple that is expressed during fruit ripening - Souleyre_2011_Phytochemistry_72_564 |
| Author(s) : Souleyre EJ , Marshall SD , Oakeshott JG , Russell RJ , Plummer KM , Newcomb RD |
| Ref : Phytochemistry , 72 :564 , 2011 |
| Abstract : Souleyre_2011_Phytochemistry_72_564 |
| ESTHER : Souleyre_2011_Phytochemistry_72_564 |
| PubMedSearch : Souleyre_2011_Phytochemistry_72_564 |
| PubMedID: 21315388 |
| Gene_locus related to this paper: 9rosa-CXE1 , 9rosa-CXE2 , 9rosa-CXE3 , 9rosa-CXE4 , 9rosa-CXE5 , 9rosa-CXE6 , 9rosa-CXE7 , 9rosa-CXE8 , 9rosa-CXE9 , 9rosa-CXE10 , 9rosa-CXE11 , 9rosa-CXE12 |