(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Hexapoda: NE > Insecta: NE > Dicondylia: NE > Pterygota: NE > Neoptera: NE > Holometabola: NE > Amphiesmenoptera: NE > Lepidoptera: NE > Glossata: NE > Neolepidoptera: NE > Heteroneura: NE > Ditrysia: NE > Obtectomera: NE > Pyraloidea: NE > Crambidae: NE > Crambinae: NE > Chilo: NE > Chilo suppressalis: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Papilio xuthus: N, E.
Pectinophora gossypiella: N, E.
Scirpophaga incertulas: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MSRNIKIVFTKLLLCFFVSGAFGRSWANHHDTTTSTTQTTPTTSPLPKNI HSDPLIVETKSGLIKGYAKTVMGREVHIFTGIPFAKPPLGPLRFRKPVPI DPWHGVLEATAMPNSCYQERYEYFPGFEGEEMWNPNTNISEDCLYLNIWV PQHLRVRHHQKKPLTEKPKVPILVWIYGGGYMSGTATLDLYKADIMASSS DVIVASMQYRVGAFGFLYLNKYFSAGSEEAPGNMGLWDQQLAIRWIKENA RAFGGDPELITLFGESAGGGSVSLHMLSPEMKGLFKRGILQSGTLNAPWS WMTGERAQDIGKVLVDDCNCNSSLLTADPSLVMDCMRGVDAKTISVQQWN SYTGILGFPSAPTVDGVFLPKDPDTMMKEGNFHNTEVLLGSNQDEGTYFL LYDFLDYFEKDGPSFLQREKFLEIVDTIFKEFSKIKREAIVFQYTDWEEI TDGYLNQKMIADVVGDYFFVCPTNYFAEILADAGVDVYYYYFTHRTSTSL WGEWMGVMHGDEMEYVFGHPLNMSLQYHTRERDLAAHIMQSFTRFALTGK PHKPDEKWPLYSRSSPHYYTYTADGTSGPAGPRGPRASACAFWNDFLNKL NELEHVPCDRAVTGPYSSVAGTTLPILLLTALATSVAL
Reference
Title: Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z Ref: Biochemical & Biophysical Research Communications, 378:269, 2009 : PubMed
Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.