(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Viridiplantae: NE > Streptophyta: NE > Streptophytina: NE > Embryophyta: NE > Tracheophyta: NE > Euphyllophyta: NE > Spermatophyta: NE > Magnoliophyta: NE > Mesangiospermae: NE > eudicotyledons: NE > Gunneridae: NE > Pentapetalae: NE > asterids: NE > lamiids: NE > Lamiales: NE > Bignoniaceae: NE > Crescentiina: NE > Tabebuia alliance: NE > Handroanthus: NE > Handroanthus impetiginosus: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MVQEKKVIDGVSGWLTVFDDGSVDRTWTGPPEVKFMSDAVPPHSDFIDGV ATADVTADQDSGLKLRIYLPEKQENDLEKFPVLLHFHGGGFCISKADWFM YYNTYTRLASAARAIVVSVYLRFAPEHRLPAACDDGLAALLWLRSVASGQ DHEPWLNEHADFNRVFLIGDSSGGNIVHQVAERAGKENLTPLRLAGAIPI HPGFCRSKRSKSELEQPETPFLTLDMVDKFLKLALPVGATKDHPITCPMG EAAPRLEEVNLPPYLYCVAEQDLVKDTQMEFYEAMKRANKEVEMFVSHGV SHSFYLNKIALEIDPKTGEETQKLFQGIKEFVNKY
Reference
Title: Genome assembly of the Pink Ipe (Handroanthus impetiginosus, Bignoniaceae), a highly valued, ecologically keystone Neotropical timber forest tree Silva-Junior OB, Grattapaglia D, Novaes E, Collevatti RG Ref: Gigascience, 7:1, 2018 : PubMed
Background: Handroanthus impetiginosus (Mart. ex DC.) Mattos is a keystone Neotropical hardwood tree widely distributed in seasonally dry tropical forests of South and Mesoamerica. Regarded as the "new mahogany," it is the second most expensive timber, the most logged species in Brazil, and currently under significant illegal trading pressure. The plant produces large amounts of quinoids, specialized metabolites with documented antitumorous and antibiotic effects. The development of genomic resources is needed to better understand and conserve the diversity of the species, to empower forensic identification of the origin of timber, and to identify genes for important metabolic compounds. Findings: The genome assembly covers 503.7 Mb (N50 = 81 316 bp), 90.4% of the 557-Mbp genome, with 13 206 scaffolds. A repeat database with 1508 sequences was developed, allowing masking of approximately 31% of the assembly. Depth of coverage indicated that consensus determination adequately removed haplotypes assembled separately due to the extensive heterozygosity of the species. Automatic gene prediction provided 31 688 structures and 35 479 messenger RNA transcripts, while external evidence supported a well-curated set of 28 603 high-confidence models (90% of total). Finally, we used the genomic sequence and the comprehensive gene content annotation to identify genes related to the production of specialized metabolites. Conclusions: This genome assembly is the first well-curated resource for a Neotropical forest tree and the first one for a member of the Bignoniaceae family, opening exceptional opportunities to empower molecular, phytochemical, and breeding studies. This work should inspire the development of similar genomic resources for the largely neglected forest trees of the mega-diverse tropical biomes.
