(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Metazoa: NE > Eumetazoa: NE > Bilateria: NE > Protostomia: NE > Ecdysozoa: NE > Panarthropoda: NE > Arthropoda: NE > Mandibulata: NE > Pancrustacea: NE > Crustacea: NE > Malacostraca: NE > Eumalacostraca: NE > Eucarida: NE > Decapoda: NE > Pleocyemata: NE > Caridea: NE > Pandaloidea: NE > Pandalidae: NE > Pandalopsis: NE > Pandalopsis japonica: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKLLFLHAIAFGFQLIVCAGELEAPVISTEEGNVAGVVEEASNGKPFHSY YGIPYATPPVGELRFKDPVPANKWKGVRDGSSMPSPCPEVPYGAAVMGIK LTAKELPGKEDCLYLNVFKPKAAQPSEGDFPVMVYIHGGGYFAGGAEEYL PHVLMSKDIILVVIQYRLGFLGFLSTEDSVMPGNYGLKDQTLALQWVQKN IQNFGGDPKRVTIFGESAGSASVHYHMLSPKTKGLFSGAIMQSGSAFASW ASSKEHKKTAKKVGSLVGCNLEEGSQPSQVMHCDRARSMAPELSSVILPS CSPESNETLPDDQLEYAVRRYNRGQTMIGVTANEGAALTQPLYGVRRNLM SELEKGYPVLGPLSIENSLNDNDSLRLANHIYNYYLGGVNLDIEHAEDMT KMISDVHFNLGFDLVSQLHSRATGESTFRYELGHRGQMSFGDFTTVDVGR HWVPHVDDLYYLFRGGPMLTPVEQPPERPTDLEAPEDLAVRNFMVTLWTN FAAHGHPTPDKSLGFVWEATNPDNLRHLALTPNPSMKGDARKEVRRFLLS LPTETNLILNPNLITENTFRTGSKRRNQDEL
Reference
Title: Two juvenile hormone esterase-like carboxylesterase cDNAs from a Pandalus shrimp (Pandalopsis japonica): cloning, tissue expression, and effects of eyestalk ablation Lee SO, Jeon JM, Oh CW, Kim YM, Kang CK, Lee DS, Mykles DL, Kim HW Ref: Comparative Biochemistry & Physiology B Biochem Mol Biol, 159:148, 2011 : PubMed
Methyl farnesoate (MF), a crustacean juvenile hormone (JH) analog, plays important roles in the regulation of a number of physiological processes such as molting, metamorphosis, and reproduction. Understanding its metabolic pathway is a key for various potential applications in crustacean aquaculture, including artificial seed production and enhancement of growth. Although the synthetic pathway of MF is well established, little is known about its degradation and recycling in crustaceans. In insects, juvenile hormone esterase (JHE), a carboxylesterase, is responsible for JH inactivation. Two cDNAs, encoding JHE-like carboxylesterases (CXEs) from the hepatopancreas and ovary of Pandalopsis japonica, were isolated by using a combination of in-silico data mining from an expressed sequence tag (EST) database and traditional PCR-based cloning. The full length Pj-CXE1 (2084bp) and Pj-CXE2 (1985bp) cDNAs encoded proteins composed of 584 and 581 amino acids, respectively. The active site sequence and domain organization of the Pj-CXEs were highly conserved, including the catalytic triad and other motifs, which suggested that both Pj-CXEs are biologically active carboxylesterases. Phylogenetic analysis of the deduced sequences of Pj-CXEs showed that both were most closely related to the JHEs from non-lepidopteran insects. End-point RT-PCR showed that Pj-CXE1 was expressed primarily in the gonad, whereas Pj-CXE2 was expressed in both the hepatopancreas and hindgut. Quantitative PCR showed that Pj-CXE1 was upregulated in the gonads by eyestalk ablation (ESA). In contrast, ESA had no significant effect on Pj-CXE2 expression in hepatopancreas or gonad. This is the first report of the cloning of two JHE-like CXE cDNAs in decapods and the upregulation of Pj-CXE1 by acute withdrawal of eyestalk neuropeptides. Further study is needed to understand the function of CXEs in MF metabolism and its regulation by eyestalk neuropeptides.
