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9bact-g3cr02

environmental samples uncultured bacterium Esterase estDL136

Comment

Other strains: environmental samples uncultured bacterium (pDL136) || EstDL136 reactivated chloramphenicol from its acetyl derivates by counteracting the chloramphenicol acetyltransferase (CAT) activity. It catalyzed the deacetylation of 1- and 3- acetyl and 1,3-diacetyl derivates.EstDL136 also acts as a chloramphenicol hydrolase.It cleaves the amide linkage at C2 of chloramphenicol and thereby confers Cm resistance to bacteria

Relationship

Family : Hormone-sensitive_lipase_like

Block : H

Position in NCBI Life Tree : uncultured bacterium

Molecular evidence

No mutation

No kinetic

No disease

No inhibitor

Database

No Genbank

Sequence

Peptide

MPLNPHVEAL LQMMAQMPAP DFSVANPAEI RAVFDNPMPL AAPPQVARVE NIAISLDGRD LDARLYVPED ADERPALMVY YHGGGWVIGT LDTHDGTCRA LAQKSGCAVL SIAYRLAPEY RYPAPAEDCY DALVWAKQNA ATLGVDGDRL AVGGDSAGGN LAAAVAIMAR DRNGPALRHQ LLIYPVTDND FTLASYAENG GGEYYLSTDG MRWFWGHYLG DTAAENAPLA AVLNVADLSG LAPATVITAE YDPLRDEGIA YAKKLDAAGV PVDAATAPGM IHGFFSMFEA VPDSWEWIER GASNLKRDLA

References

Title : Crystal structure of chloramphenicol-metabolizing enzyme EstDL136 from a metagenome - Kim_2019_PLoS.One_14_e0210298
Author(s) : Kim SH , Kang PA , Han K , Lee SW , Rhee S
Ref : PLoS ONE , 14 :e0210298 , 2019
Abstract : Kim_2019_PLoS.One_14_e0210298
ESTHER : Kim_2019_PLoS.One_14_e0210298
PubMedSearch : Kim_2019_PLoS.One_14_e0210298
PubMedID: 30645605
Gene_locus related to this paper: 9bact-g3cr02

Title : Inactivation of chloramphenicol and florfenicol by a novel chloramphenicol hydrolase - Tao_2012_Appl.Environ.Microbiol_78_6295
Author(s) : Tao W , Lee MH , Wu J , Kim NH , Kim JC , Chung E , Hwang EC , Lee SW
Ref : Applied Environmental Microbiology , 78 :6295 , 2012
Abstract : Tao_2012_Appl.Environ.Microbiol_78_6295
ESTHER : Tao_2012_Appl.Environ.Microbiol_78_6295
PubMedSearch : Tao_2012_Appl.Environ.Microbiol_78_6295
PubMedID: 22752166
Gene_locus related to this paper: 9bact-g3cr00 , 9bact-g3cr02

Title : Characterization of two metagenome-derived esterases that reactivate chloramphenicol by counteracting chloramphenicol acetyltransferase - Tao_2011_J.Microbiol.Biotechnol_21_1203
Author(s) : Tao W , Lee MH , Yoon MY , Kim JC , Malhotra S , Wu J , Hwang EC , Lee SW
Ref : J Microbiol Biotechnol , 21 :1203 , 2011
Abstract : Tao_2011_J.Microbiol.Biotechnol_21_1203
ESTHER : Tao_2011_J.Microbiol.Biotechnol_21_1203
PubMedSearch : Tao_2011_J.Microbiol.Biotechnol_21_1203
PubMedID: 22210605
Gene_locus related to this paper: 9bact-g3cr00 , 9bact-g3cr02