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Gene_locus Report for: 9bact-g1apt8

uncultured bacterium LipC12, a true lipase isolated through a metagenomics approach

Relationship
Family|Bacterial_lip_FamI.1
Block| L
Position in NCBI Life Tree|uncultured bacterium
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > environmental samples: N E > uncultured bacterium: N E
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data


Molecular evidence
Database
No mutation
1 structure:
6CL4: LipC12 - Lipase from metagenomics
No kinetic





No Substrate
No inhibitor
1 Genbank : JF417979
1 UniProt : G1APT8
1 Structure : 6CL4
1 UniProt : G1APT8
1 Interpro : G1APT8
1 Pfam : G1APT8
1 PIRSF : G1APT8
1 SUPERFAM : G1APT8
Sequence
Peptide
Graphical view for this peptide sequence: 9bact-g1apt8
Colored MSA for Bacterial_lip_FamI.1 (raw) 
MSASSLKFPIVLVHGLLGFDKIGGIYPYFYGIKEALEKAGAKVYIATLSA
LNSNELRGEQLLEFVRKVQAETGAAKVNLIGHSQGPLACRYVAATHPELI
ASVTSVNGVNHGSEVADLVRLALTPGRLPESIANAAMSAFGQLLSALAGS
PRLPQSGIEALEALTSEGVAAFNNKYPQGLPAEWGGEGKELVNGVYYYSW
SGVIDYNPLHQGANNLDPLHVAMLAFSILFTNERFQNDGLVGRYSSHLGK
VIGSDYSMDHVDAINQLAGVVANNTDPVQLFVEHVARLKSKGL
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
MSASSLKFPIVLVHGLLGFDKIGGIYPYFYGIKEALEKAGAKVYIATLSA
LNSNELRGEQLLEFVRKVQAETGAAKVNLIGHSQGPLACRYVAATHPELI
ASVTSVNGVNHGSEVADLVRLALTPGRLPESIANAAMSAFGQLLSALAGS
PRLPQSGIEALEALTSEGVAAFNNKYPQGLPAEWGGEGKELVNGVYYYSW
SGVIDYNPLHQGANNLDPLHVAMLAFSILFTNERFQNDGLVGRYSSHLGK
VIGSDYSMDHVDAINQLAGVVANNTDPVQLFVEHVARLKSKGL

References
    Title: Structure solution and analyses of the first true lipase obtained from metagenomics indicate potential for increased thermostability
    Martini VP, Krieger N, Glogauer A, Souza EM, Iulek J
    Ref: N Biotechnol, 53:65, 2019 : PubMed

            

    Title: Crystallization and preliminary crystallographic analysis of LipC12, a true lipase isolated through a metagenomics approach
    Martini VP, Glogauer A, Iulek J, Souza EM, Pedrosa FO, Krieger N
    Ref: Acta Crystallographica Sect F Struct Biol Cryst Commun, 68:175, 2012 : PubMed

            

    Title: Identification and characterization of a new true lipase isolated through metagenomic approach
    Glogauer A, Martini VP, Faoro H, Couto GH, Muller-Santos M, Monteiro RA, Mitchell DA, de Souza EM, Pedrosa FO, Krieger N
    Ref: Microb Cell Fact, 10:54, 2011 : PubMed

            


Other Papers

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
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