Gene_Locus Report

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Gene_locus Report for: 9bact-KP212148

Uncultured bacterium Dienelactone Hydrolase-like Promiscuous Phosphotriesterase P91 from Metagenomic Libraries

Relationship

Family	\|	Dienelactone_hydrolase
Block	\|	X
Position in NCBI Life Tree	\|	uncultured bacterium

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > environmental samples: N E > uncultured bacterium: N E

There are 310 a/b hydrolases in uncultured bacterium view in a: Table

5_AlphaBeta_hydrolase : 9bact-estC55.165uncultured bacterium. Lipolytic protein estC55-165, 9bact-estC55.241uncultured bacterium. Lipolytic protein estC55-241, 9bact-estC76.137uncultured bacterium. Lipolytic protein estC76-137.
6_AlphaBeta_hydrolase : 9bact-d8v1i9uncultured bacterium Esterase/lipase clone EM3L6, 9bact-d8v1i5uncultured bacterium Esterase/lipase clone EM3L1, 9bact-i3rtt4uncultured bacterium EstL28 cold-active Esterase, 9bact-a0a0n9qma1uncultured bacterium esterase est1, 9bact-a0a1s5qki8uncultured bacterium EH127 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qm34uncultured bacterium EH113 Alpha/beta hydrolase fold carboxyl esterase, 9bact-r9qzg0uncultured bacterium EH109 Hybrid C-C meta-cleavage hydrolase-carboxylesterase, 9gamm-KY483646Uncultured bacterium EH57 Alpha/beta hydrolase fold, 9bact-KY458160Uncultured bacterium EH53 clone 2085D pCC1FOS Lip088, 9bact-a0a1s5qm38uncultured bacterium, EH26,clone MGS-ElMaxE1 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a0h4tgu6uncultured bacterium EH58 Alpha/beta hydrolase fold protein LIPESV12_9 LIPESV12_24, 9zzzz-g3crf3uncultured bacteria Metagenomic AB hydrolase-1 domain-containing protein, 9bact-i6yrg4

uncultured bacterium Est816 N-Acyl homoserine lactone and pyrethroid degrading enzyme, 9bact-a2a1y4uncultured bacterium putative prolyl aminopeptidase, 9bact-d3ykv3uncultured bacterium Lip, 9bact-q3ymm0uncultured bacterium lipase/esterase, 9bact-q3ymm6uncultured bacterium lipase/esterase, 9bact-q3ymm8uncultured bacterium lipase/esterase, 9bact-q3ymm9uncultured bacterium lipase/esterase, 9bact-MGS-M2

uncultured bacterium EH107 alpha/beta hydrolase enzyme MGS-M2, 9bact-estC55.25uncultured bacterium. Lipolytic protein estC55-25, 9bact-estC55.76uncultured bacterium. Lipolytic protein estC55-76, 9bact-estC55.20uncultured bacterium. Lipolytic protein, 9bact-estC55.4n1uncultured bacterium. Lipolytic protein, 9bact-estC76.263uncultured bacterium. Lipolytic protein estC55-100 estC76-263, 9bact-estC55.227uncultured bacterium. Lipolytic protein estC55-227, 9bact-estC55.159uncultured bacterium. Lipolytic protein estC55-159, 9bact-estC55.51uncultured bacterium. Lipolytic protein estC55-51, 9bact-estC55.31uncultured bacterium. Lipolytic protein estC55-31, 9bact-estC55.215uncultured bacterium. Lipolytic protein estC55-215, 9bact-estC55.244uncultured bacterium. Lipolytic protein estC55-244, 9bact-estC55.81uncultured bacterium. Lipolytic protein estC55-81, 9bact-estC55.15uncultured bacterium. Lipolytic protein estC55-15, 9bact-estC55.231uncultured bacterium. Lipolytic protein estC55-231, 9bact-estC76.266n2uncultured bacterium. Lipolytic protein estC76-28_2 estC76-266_2, 9bact-estC76.28n1uncultured bacterium. Lipolytic protein estC76.28_1, 9bact-estC76.177uncultured bacterium. Lipolytic protein, 9bact-estC76.266n1uncultured bacterium. Lipolytic protein estC76-266_1, 9bact-estC76.248uncultured bacterium. Lipolytic protein estC76-248.
A85-EsteraseD-FGH : 9bact-m9vy17uncultured bacterium FAE1 Feruloyl esterase (FAE-1) sourced from a termite hindgut, 9bact-KY458159Uncultured bacterium EH44 clone 826H pCC1FOS Lip061, 9bact-b0fwn3uncultured bacterium Esterase A, 9bact-b5au41uncultured bacterium Esterase.
A85-Feruloyl-Esterase : 9bact-MW804671uncultured bacterium Esterase EstQ7 MW804671, 9bact-a0a0k0l6g3uncultured bacterium Esterase AS-Trib27, 9bact-a0a1y0bdl3uncultured bacterium Esterase D591, 9bact-a0a140fva9uncultured bacterium ESTGX3.
A85-IroE-IroD-Fes-Yiel : 9bact-g3c5s8uncultured bacterium. Ferric enterobactin esterase-related protein.
ABHD6-Lip : 9bact-a0a1s5ql22uncultured bacterium EH114 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qly6uncultured bacterium EH65 Alpha/beta hydrolase fold carboxyl esterase, 9bact-d8v1i7uncultured bacterium Esterase/lipase MhpC clone EM3L3, 9bact-r9qzf7uncultured bacterium EH55 Hybrid C-C meta-cleavage hydrolase-carboxylesterase.
ABHD11-Acetyl_transferase : 9bact-a0a2s1gux0

esterase fromdeep-sea hydrothermal vent of the East Pacific Rise (EprEst) uncultured bacterium pet-28a est-OKK esterase, 9bact-a0a0k1z4z5uncultured bacterium Alpha/beta hydrolase fold protein LIPESV12_26, 9bact-g3c5s4uncultured bacterium. Alpha/beta hydrolase fold protein, 9bact-estC55.96uncultured bacterium. Lipolytic protein.
abh_upf0017 : 9bact-q3ymm2uncultured bacterium Lipase/esterase.
abh_upf00227 : 9bact-h8xw46uncultured bacterium. Carboxylesterase.
Acetyl-esterase_deacetylase : 9bact-KY458164Uncultured bacterium EH70 clone 27C11 pCC1FOS Lip113.
Aclacinomycin-methylesterase_RdmC : 9bact-a9ukk2uncultured bacterium Lipase/esterase, 9bact-b0l3i3uncultured bacterium Lipase/esterase, 9bact-b8y566uncultured bacterium Lipolytic enzyme, 9bact-c3rww3uncultured bacterium Putative lipase/esterase, uncba-Q9KIU0uncultured bacterium esterase, 9bact-g3c5t0uncultured bacterium. Lipase/esterase, 9bact-g3c5s9uncultured bacterium. Lipase/esterase, 9bact-estC55.197uncultured bacterium. Lipolytic protein.
AlphaBeta_hydrolase : 9bact-Aii810uncultured bacterium Cold-adapted N-acylhomoserine lactonase Aii810, 9bact-KY458167uncultured bacterium EH130 clone 83E4 pCC1FOS Lip139, 9bact-KY203031uncultured bacterium EH74 clone 00041_62_12ROV11 esterase/lipase, 9bact-estC55.12uncultured bacterium. Lipolytic protein estC55-12.
Bacterial_Est97 : 9bact-Est97

uncultured bacterium cold-adapted esterase from an Arctic intertidal metagenomic library.
Bacterial_esterase : uncba-Q848M3uncultured bacterium hypothetical protein.
Bacterial_lip_FamI.1 : 9bact-g1apt8

uncultured bacterium LipC12, a true lipase isolated through a metagenomics approach.
Bacterial_lip_FamI.2 : 9bact-h2d5p3uncultured bacterium Lipase lipD SMlipD.
Bacterial_lip_FamI.3 : 9bact-g9hwf0uncultured bacterium Lipase lipB SMlipB, 9bact-q6xci1uncultured bacterium lipb, 9bact-q6xci2uncultured bacterium lipa.
Bacterial_lip_FamI.5 : 9bact-a4uz10uncultured bacterium PLA depolymerase pLA-M4 pLAM4.
Bact_LipEH166_FamXII : 9bact-b1pz93uncultured bacterium Lipase.
BD-FAE : 9bact-a0a1c9t7d1Uncultured bacterium clone C5 carboxylesterase (est), 9prot-EH41Uncultured bacterium EH41 SRA059294 esterase/lipase, 9bact-CEUbrbuncultured bacterium carbamate degrading enzyme active on Impranil poly-urethane polyester polyurethane PUR, 9zzzz-g3crg0uncultured bacteria Metagenomic Abhydrolase_3 domain-containing protein, 9bact-q6rjl2uncultured bacterium lipase/esterase, 9bact-MGS-M1

uncultured bacterium EH115 alpha/beta hydrolase enzyme MGS-M1, 9bact-a0a345g0q2uncultured bacterium. Est903 Putative esterase, 9bact-estC55.253uncultured bacterium. Lipolytic protein estC55-253, 9bact-estC55.57uncultured bacterium. Lipolytic protein.
BioG_Pimeloyl-ACP-methyl-esterase : 9bact-w1i648environmental samples uncultured bacterium Uncultured bacterium extrachromosomal DNA RGI01476.
BioH : 9bact-q3ymm4uncultured bacterium lipase/esterase, 9bact-q6a3q9uncultured bacterium lip1 protein, 9bact-estC76.202uncultured bacterium. Lipolytic protein estC76-202.
CarbLipBact_2 : 9bact-KY203034Uncultured bacterium EH111 clone 00002_36_12ROV11 esterase/lipase, 9bact-k2dnl8uncultured bacterium. Uncharacterized protein, 9bact-k2f369uncultured bacterium. Uncharacterized protein, 9bact-k2f264uncultured bacterium. Uncharacterized protein, 9bact-k2gak8uncultured bacterium. Uncharacterized protein, 9bact-k2age0uncultured bacterium. Uncharacterized protein, 9bact-k2fcq4uncultured bacterium. Uncharacterized protein, 9bact-k2fvb3uncultured bacterium. Uncharacterized protein, 9bact-r9rev7uncultured bacterium. Esterase.
Carbon-carbon_bond_hydrolase : 9bact-a0a1s5qkm4uncultured bacterium EH63 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qke6uncultured bacterium EH38 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkf3uncultured bacterium EH36 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkl2Uncultured bacterium, EH33, clone MGS-MesE6 alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qmi7Uncultured bacterium, EH17, clone MGS-AqE1 alpha/beta hydrolase fold carboxyl esterase, 9bact-MChE2

Uncultured bacterium MGS-MChE2,alpha/beta hydrolase enzyme from sediment sample of lagoon of Mar Chica, Morocco, 9bact-c6kti0uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6ktz1uncultured bacterium 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, 9bact-c6ku85uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kub4uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kud7uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6kwi7uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-c6l0y5uncultured bacterium 2-hydroxymuconic semialdehyde hydrolase, 9bact-estC55.2uncultured bacterium. Lipolytic protein, 9bact-estC55.97uncultured bacterium. Lipolytic protein estC55-97.
Carboxymethylbutenolide_lactonase : 9bact-estC55.34uncultured bacterium. Lipolytic protein estC55-34.
Carb_B_Bacteria : 9bact-102Pee006uncultured bacterium 176763, pEE006 102 YNP6_02150 Environmental sample, 9bact-a0a1s5y1h9uncultured bacterium. Carboxylic ester hydrolase, 9bact-a0a1s5qkj8uncultured bacterium EH139 Carboxylic ester hydrolase, 9bact-a0a1s5qkg4uncultured bacterium EH64 Carboxylic ester hydrolase, 9actn-KY010298Uncultured bacterium, EH20 carboxyl esterase, 9bact-b8y554uncultured bacterium Lipolytic enzyme, 9bact-c6ku06uncultured bacterium Carboxylesterase, 9bact-c7f9k0uncultured bacterium Lipase/esterase, 9bact-d2xv58uncultured bacterium Carboxylesterase, 9bact-g1ard1uncultured bacterium EstDL30, 9bact-h9lcg9uncultured bacterium Est2, 9bact-i2b2s6uncultured bacterium Carboxylesterase, 9bact-q3ymm5uncultured bacterium lipase/esterase, 9bact-j7i2s6uncultured bacterium Carboxylesterase type B, 9bact-j7hzn3uncultured bacterium Thermostable carboxylesterase, 9bact-j7hzn6uncultured bacterium Thermostable carboxylesterase, 9bact-j7i2s2uncultured bacterium Thermostable carboxylesterase, 9bact-j7i5y7uncultured bacterium Carboxylesterase type B, 9bact-j7i6j5uncultured bacterium Carboxylesterase type B, 9bact-k2eq93uncultured bacterium Uncharacterized protein, 9bact-s5tby8uncultured bacterium Esterase, 9bact-a0a097i5b4uncultured bacterium. Carboxylic ester hydrolase, 9bact-a0a0a7eq69uncultured bacterium. Carboxylic ester hydrolase, 9bact-estC76.136uncultured bacterium. Carboxylic ester hydrolase estC76-136, 9bact-estC55.118uncultured bacterium. Carboxylic ester hydrolase estC55-118, 9bact-estC55.62uncultured bacterium. Carboxylic ester hydrolase estC55-62, 9bact-estC55.52uncultured bacterium. Carboxylic ester hydrolase estC55-52, 9bact-estC55.3uncultured bacterium. Carboxylic ester hydrolase estC55-3.
Chlorophyllase : 9bact-a0a1b2ypb1uncultured bacterium. Uncharacterized protein, 9bact-b5au40uncultured bacterium. Lipase, 9bact-estC55.24uncultured bacterium. Lipolytic protein estC55-24.
Duf_3089 : 9bact-MT859404Uncultured bacterium Est3S from cow rumen metagenomic library with organosphosphorus insecticides degrading capability, 9bact-d5l5q1uncultured bacterium EstGK1, 9bact-d5l5q2uncultured bacterium EstZ3 EstGK1, 9bact-d8v1i6uncultured bacterium Esterase/lipase clone EM3L2, 9bact-e2d695uncultured bacterium EstD2, 9bact-q0gpb3uncultured bacterium Est5S, 9bact-a0a077l869uncultured bacterium. Uncharacterized protein, 9bact-j7hz76uncultured bacterium. Esterase, 9bact-j7i5z1uncultured bacterium. Esterase, 9bact-j7i6j9uncultured bacterium. Esterase, 9bact-j7i6k3uncultured bacterium. Esterase, 9bact-k2cj78uncultured bacterium. Uncharacterized protein, 9bact-k9ryb0uncultured bacterium. EstWSD, 9bact-a0a088s6n7uncultured bacterium. Esterase Est10.
Epoxide_hydrolase : 9bact-a0a1u9wz52

uncultured bacterium CH65-eh Alpha/beta epoxide hydrolase, 9bact-a0a1u9wz58

uncultured bacterium Sibe-eh Alpha/beta epoxide hydrolase, 9bact-a0a1s5qkk8Uncultured bacterium, EH32, clone MGS-MilE2 alpha/beta hydrolase fold carboxyl esterase, 9bact-a8wbx9uncultured bacterium Epoxide hydrolase, 9bact-estC55.19n2uncultured bacterium. Lipolytic protein, 9bact-estC55.8n2uncultured bacterium. Lipolytic protein.
Est-OsmC : 9bact-g3c5t8uncultured bacterium. esterase is found in n-term of an OsmC/Ohr family domain.
Est9X : 9bact-j9vdv8uncultured bacterium Esterase.
Esterase_phb : 9bact-d8v1i8uncultured bacterium Esterase/lipase EM3L4, 9bact-estC55.77uncultured bacterium. Lipolytic protein estC55-77.
FAE-Bacterial-promiscuous : 9bact-h8xw45uncultured bacterium. Carboxylesterase, 9bact-estC76.221uncultured bacterium. Lipolytic protein.
FaeC : 9bact-a0a0u2zp04uncultured bacterium. Ricin-type beta-trefoil lectin domain protein, 9bact-estC55.42uncultured bacterium. Lipolytic protein estC55-42.
Fungal-Bact_LIP : 9bact-estC55.154uncultured bacterium. Lipolytic protein estC55-154, 9bact-b0ln78uncultured bacterium Esterase, uncba-Q9KIU2uncultured bacterium esterase.
GTSAGmotif : 9bact-a0a142j6i6

uncultured bacterium esterase PMGL2 with GCSAG motif from a permafrost metagenomic library., 9bact-b8y553uncultured bacterium Lipolytic enzyme, 9bact-b1pz92uncultured bacterium Esterase, 9bact-d8v1j5uncultured bacterium Esterase, 9bact-d8v1j4uncultured bacterium Esterase, 9bact-d8v1j3uncultured bacterium Esterase, 9bact-d7nj22uncultured bacterium alkaline-stable lipase, 9bact-d5mt93uncultured bacterium Esterase, 9bact-a3rgw9uncultured bacterium Putative esterase, 9bact-h6bdx2uncultured bacterium Lipolytic enzyme, 9bact-b8y558uncultured bacterium Lipolytic enzyme, 9bact-b8y563uncultured bacterium Lipolytic enzyme.
Haloalkane_dehalogenase-HLD1 : 9bact-v5ln96uncultured bacterium Haloalkane dehalogenase Cluster B eHLD-B, 9bact-v5llk8uncultured bacterium. Haloalkane dehalogenase dhmA, 9bact-v5llz0uncultured bacterium. Haloalkane dehalogenase.
Haloalkane_dehalogenase-HLD2 : 9bact-AncHLD3

Putative ancestral haloalkane dehalogenase AncHLD3 (node 3), 9bact-AncHLD2

Putative ancestral haloalkane dehalogenase AncHLD2 (node 2), 9bact-AncHLD5

Putative ancestral haloalkane dehalogenase AncHLD5 (node 5), 9bact-v5llz5uncultured bacterium Haloalkane dehalogenase Cluster C eHLD-C.
HNLyase_Bact : 9bact-c7f9k4uncultured bacterium Lipase/esterase, 9bact-estC55.167uncultured bacterium. Lipolytic protein estC55-167.
Homoserine_transacetylase : 9bact-a0a1b1h1k0

uncultured bacterium; Alteromonas macleodii; Alteromonas marina, Homoserine O-acetyltransferase Est22 esterase not transferase, 9bact-g3c5t3uncultured bacterium. Lipase/esterase.
Hormone-sensitive_lipase_like : 9bact-a0a1c9t7d3Uncultured bacterium clone C9 carboxylesterase (est), 9bact-PersiLipase1uncultured bacterium alkaline, thermostable, detergent compatible and organic solvent tolerant lipase PersiLipase1, 9bact-EH0

uncultured bacterium ester-hydrolase EH0 from the metagenome of Sorghum bicolor rhizosphere from the Henfaes research centre (Gwynedd Wales), 9bact-b8y561uncultured bacterium Lipolytic enzyme lip10, 9bact-estGX1uncultured bacterium Esterase GX1 estGX1, 9bact-EstATIIuncultured bacterium; Pseudomonas sp. Thermophilic, psychrostable heavy metal-resistant Red Sea Brine Pool Esterase EstATII, 9bact-MZ484407Uncultured bacterium clone YH-E8 family IV esterase (est8L), 9bact-a0a0n9qch2uncultured bacterium Halotolerant esterase, 9bact-Est8.6Y9K

Esterase EST8 with transacylase activity (different from Est8 4YPV), 9bact-a9ukj9uncultured bacterium Lipase/esterase, 9bact-g8enl6uncultured bacterium moderately thermophilic solvent-tolerant esterase, 9bact-a0a1x9zm83uncultured bacterium RPSP24 esterase, 9bact-bol3I1uncultured bacterium. Lipase/esterase, 9zzzz-a0a2k8jn75

uncultured bacterium ester-hydrolase EH3 from the metagenome of marine sediments at Milazzo harbor (Sicily, Italy), 9zzzz-a0a2k8jt94uncultured bacterium EH2 Uncharacterized protein, 9bact-a0a1s5ql04EH67 uncultured bacterium Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY458168Uncultured bacterium EH48 clone 38H9 pCC1FOS Lip142, 9bact-t1vzd5uncultured bacterium EH42 clone L-Tri-RimGill-1 Esterase, 9prot-SRP030024Uncultured bacterium EH35 clone Alpha/beta hydrolase fold carboxyl esterase, 9prot-KY019260Uncultured bacterium, EH29 clone Alpha/beta hydrolase fold carboxyl esterase, 9prot-KY010302Uncultured bacterium, EH22, clone Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY010297Uncultured bacterium, EH15, clone Alpha/beta hydrolase fold carboxyl esterase EstA1, 9bact-KY483642Uncultured bacterium, EH14, clone Alpha/beta hydrolase fold carboxyl esterase, 9bact-a0a1s5qkj1Uncultured bacterium, EH12, clone MGS-BizE3 Alpha/beta hydrolase fold carboxyl esterase, 9bact-KY010299Uncultured bacterium, EH11, KY010299 alpha/beta hydrolase fold, 9bact-KY483648Uncultured bacterium, EH9, KY483648 alpha/beta hydrolase fold, 9bact-a0a1s5qf1Uncultured bacterium, EH4, clone MGS-MilE5 alpha/beta hydrolase fold, 9bact-a0a1s5qkg0uncultured bacterium clone MGS-ElMaxE6 EH5 Alpha/beta hydrolase fold carboxyl esterase, 9zzzz-g3crd1uncultured bacteria Metagenomic carboxyl esterase Est06, 9bact-H6BDX1

uncultured bacterium esterase Est22, 9bact-MilE3

Uncultured bacterium MGS-MilE3,EH37 alpha/beta hydrolase enzyme from sediment sample of Milazzo Harbor, Italy, 9bact-LAE6

Uncultured bacterium esterase LAE6 EH1 EH1AB1 alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain, 9bact-e7djy5

Uncultured bacterium feruloyl esterase haloalkalitolerant esterase from a soil metagenomic library Feruloyl Esterase EstF27, 9bact-Q0GMU1

Uncultured bacterium esterase/lipase (estE7) gene, complete cds, 9bact-Q0GMU2

Uncultured bacterium clone pUE5 esterase/lipase (estE5), 9bact-q3yjm9uncultured bacterium lipase, 9bact-q3ymm7uncultured bacterium lipase/esterase, 9bact-q3ymn0uncultured bacterium lipase/esterase, 9bact-q4tzq3

uncultured bacterium esterase Est25, 9bact-q6rjl0uncultured bacterium lipase/esterase, 9bact-q6rjm3uncultured bacterium lipase/esterase, bacte-esterUncultured bacterium plasmid pAH116 esterase gene, complete cds, 9bact-d7nj24uncultured bacterium Lipase/esterase, 9bact-m1plb8uncultured bacterium Esterase/lipase protein, 9bact-g3cr00uncultured bacterium. Esterase estDL26, 9bact-a0a0k0l6c0uncultured bacterium. Lipase/esterase AS-Trib6, 9bact-b8y559environmental samples uncultured bacterium Lipolytic enzyme, 9bact-b0l3i6environmental samples uncultured bacterium Lipase/esterase, 9bact-b3vp83environmental samples uncultured bacterium; Bacillus firmus Lipase/esterase, 9bact-a0a0k0l6e1environmental samples uncultured bacterium Lipase/esterase AS-Trib20-ORF2, 9bact-b0l3i4environmental samples uncultured bacterium Lipase/esterase, 9bact-b8y564environmental samples uncultured bacterium Lipolytic enzyme, 9bact-h6bdx3environmental samples uncultured bacterium Lipolytic enzyme, 9bact-k7pez9environmental samples uncultured bacterium Lipolytic enzyme SBLip2, 9bact-b8y560environmental samples uncultured bacterium Lipolytic enzyme, 9bact-a0a0k0l6e0environmental samples uncultured bacterium Lipase/esterase AS-Trib20-ORF1, 9bact-a0a0d5w6z2environmental samples uncultured bacterium Esterase EstGX2, 9bact-k7pfj8environmental samples uncultured bacterium Lipolytic enzyme SBLip5, 9bact-a0a0k0l6j0environmental samples uncultured bacterium Alpha/beta hydrolase fold-3 domain-containing protein AS-Trib23, 9bact-a0a0k0l6d5environmental samples uncultured bacterium Alpha/beta hydrolase AS-Trib12, 9bact-g3cr02

environmental samples uncultured bacterium Esterase estDL136, 9bact-estC55.268uncultured bacterium. Lipolytic protein estC55-268, 9bact-estC55.145uncultured bacterium. Lipolytic protein estC55-145, 9bact-estC55.78uncultured bacterium. Lipolytic protein estC55-78, 9bact-estC55.8n1uncultured bacterium; Chloroflexi bacterium enrichment. Lipolytic protein estC55-8_1 IS12, 9bact-IS10Chloroflexi bacterium enrichment culture clone L1N22 alpha/beta hydrolase IS10, 9bact-estC55.56uncultured bacterium. Lipolytic protein estC55-56, 9bact-estC55.60uncultured bacterium. Lipolytic protein estC55-60, 9bact-estC55.5uncultured bacterium. Lipolytic protein estC55-5, 9bact-estC55.19n1uncultured bacterium. Lipolytic protein estC55-19_1, 9bact-estC55.229uncultured bacterium. Lipolytic protein estC55-229, 9bact-estC55.23uncultured bacterium. Lipolytic protein, 9bact-estC76.135uncultured bacterium. Lipolytic protein estC76-135.
Hydrolase-2_PEP : 9bact-k2ehy0uncultured bacterium. Esterase/lipase/thioesterase family protein.
Hydrolase_RBBP9_YdeN : 9bact-q6jwt1uncultured bacterium hypothetical protein cxs042.
Lipase_2 : 9bact-estC55.235uncultured bacterium. Lipolytic protein estC55-235, 9bact-estC55.90uncultured bacterium. Lipolytic protein estC55-90, 9bact-estC55.105uncultured bacterium. Lipolytic protein estC55-105, 9bact-estC55.151uncultured bacterium. Lipolytic protein estC55-151, 9bact-estC55.71uncultured bacterium. Lipolytic protein estC55-71, 9bact-estC55.88uncultured bacterium. Lipolytic protein estC55-88.
Lipase_bact_N_lipase : 9bact-estC55.102uncultured bacterium. Lipolytic protein estC55-102.
Monoglyceridelipase_lysophospholip : 9bact-a0a1s5qlx2uncultured bacterium EH94 Alpha/beta hydrolase fold carboxyl esterase, 9bact-a4uz14uncultured bacterium PLA depolymerase pLA-M9 pLAM9.
NLS3-Tex30 : 9bact-k1ymn0uncultured bacterium Uncharacterized protein.
PAF-Acetylhydrolase : 9bact-Lip114Uncultured bacterium clone EH40 53D4 pCC1FOS Lip114.
Pectinacetylesterase-Notum : 9bact-h9ldb8uncultured bacterium. Est5, 9bact-g3c5s0uncultured bacterium. Pectinacetylesterase/lipase/esterase, 9bact-k7qe48uncultured bacterium. EH80 LipT.
Peptidase_S37 : 9bact-k1yi15uncultured bacterium; Rikenellaceae bacterium. Uncharacterized protein.
PGAP1 : 9bact-d8v1j0uncultured bacterium Esterase/lipase clone EM3L7, 9bact-c7f9k2uncultured bacterium Lipase/esterase.
Polyesterase-lipase-cutinase : 9bact-c3ryl0

uncultured bacterium Polyethylene terephthalate hydrolyzing lipase PET2 LipIAF5-2, 9bact-estC55.95uncultured bacterium. Lipolytic protein estC55-95.
Polyesterase-MGS0156-like : 9zzzz-A0A0G3FEJ8

uncultured bacteria Metagenomic carboxyl esterase MGS0156.
Prolyl_oligopeptidase_S9 : 9bact-KY203037uncultured bacterium EH120 clone 00152_2_12ROV11 esterase/lipase, 9bact-q6wlc7uncultured bacterium csw020.
Tannase : 9bact-e7d7j5uncultured bacterium Multi-fuction esterase tannase tan410, 9bact-estC55.156uncultured bacterium. Lipolytic protein estC55-156, 9bact-estC55.169uncultured bacterium. Lipolytic protein estC55-169, 9bact-estC55.234uncultured bacterium. Lipolytic protein estC55-234.
UCP031982 : 9bact-d8v1j6uncultured bacterium Esterase/lipase, 9bact-estC55.13uncultured bacterium. Lipolytic protein estC55-13

Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data

Molecular evidence		Database
No mutation 1 structure: 4ZI5: Crystal Structure of Dienelactone Hydrolase-like Promiscuous Phospotriesterase P91 from Metagenomic Libraries No kinetic 2 substrates: Fluorescein-di(diethylphosphate), Paraoxon 1 inhbitor: Paraoxon		1 Genbank : KP212148 1 UniProt : A0A141FAA2 1 Structure : 4ZI5 1 UniProt : A0A141FAA2 1 Interpro : A0A141FAA2 1 Pfam : A0A141FAA2 1 PIRSF : A0A141FAA2 1 SUPERFAM : A0A141FAA2

Sequence

Peptide

Graphical view for this peptide sequence: 9bact-KP212148
Colored MSA for Dienelactone_hydrolase (raw)
MASWSHPQFEKGAMTARKVDYTDGATRCIGEFHWDEGKSGPRPGVVVFPE AFGLNDHAKERARRLADLGFAALAADMHGDAQVFDAASLSSTIQGYYGDR AHWRRRAQAALDALTAQPEVDGSKVAAIGFCFGGATCLELARTGAPLTAI VTFHGGLLPEMAGDAGRIQSSVLVCHGADDPLVQDETMKAVMDEFRRDKV DWQVLYLGNAVHSFTDPLAGSHGIPGLAYDATAEARSWTAMCNLFSELFG
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA
MASWSHPQFEKGAMTARKVDYTDGATRCIGEFHWDEGKSGPRPGVVVFPE
AFGLNDHAKERARRLADLGFAALAADMHGDAQVFDAASLSSTIQGYYGDR
AHWRRRAQAALDALTAQPEVDGSKVAAIGFCFGGATCLELARTGAPLTAI
VTFHGGLLPEMAGDAGRIQSSVLVCHGADDPLVQDETMKAVMDEFRRDKV
DWQVLYLGNAVHSFTDPLAGSHGIPGLAYDATAEARSWTAMCNLFSELFG

References

Title:

Schnettler JD, Klein OJ, Kaminski TS, Colin PY, Hollfelder F

Ref:

PubMed

ESTHER: Schnettler_2022_J.Am.Chem.Soc__
PubMedSearch: Schnettler 2022 J.Am.Chem.Soc
PubMedID: 36583539
Gene_locus related to this paper: 9bact-KP212148
Inhibitor(s) related to this paper: Paraoxon

Abstract

Finding new mechanistic solutions for biocatalytic challenges is key in the evolutionary adaptation of enzymes, as well as in devising new catalysts. The recent release of man-made substances into the environment provides a dynamic testing ground for observing biocatalytic innovation at play. Phosphate triesters, used as pesticides, have only recently been introduced into the environment, where they have no natural counterpart. Enzymes have rapidly evolved to hydrolyze phosphate triesters in response to this challenge, converging onto the same mechanistic solution, which requires bivalent cations as a cofactor for catalysis. In contrast, the previously identified metagenomic promiscuous hydrolase P91, a homologue of acetylcholinesterase, achieves slow phosphotriester hydrolysis mediated by a metal-independent Cys-His-Asp triad. Here, we probe the evolvability of this new catalytic motif by subjecting P91 to directed evolution. By combining a focused library approach with the ultrahigh throughput of droplet microfluidics, we increase P91's activity by a factor of =360 (to a k(cat)/K(M) of =7 x 10(5) M(-1) s(-1)) in only two rounds of evolution, rivaling the catalytic efficiencies of naturally evolved, metal-dependent phosphotriesterases. Unlike its homologue acetylcholinesterase, P91 does not suffer suicide inhibition; instead, fast dephosphorylation rates make the formation of the covalent adduct rather than its hydrolysis rate-limiting. This step is improved by directed evolution, with intermediate formation accelerated by 2 orders of magnitude. Combining focused, combinatorial libraries with the ultrahigh throughput of droplet microfluidics can be leveraged to identify and enhance mechanistic strategies that have not reached high efficiency in nature, resulting in alternative reagents with novel catalytic machineries.

Title:

Schnettler-Fernandez D, Klein OJ, Kaminski TS, Colin PY, Hollfelder F

Ref:

PubMed

ESTHER: Schnettler-Fernandez_2022_Biorxiv__
PubMedSearch: Schnettler-Fernandez 2022 Biorxiv
Gene_locus related to this paper: 9bact-KP212148

Abstract

The recent massive release of new, man-made substances into the environment requires bioremediation, but a very limited number of enzymes evolved in response are available. When environments have not encountered the potentially hazardous materials in their evolutionary history, existing enzymes have to be repurposed. The recruitment of accidental, typically low-level promiscuous activities provides a head start that, after gene duplication, can adapt and provide a selectable advantage. This evolutionary scenario raises the question whether it is possible to adaptively improve the low-level activity of enzymes recruited from non- (or only recently) contaminated environments quickly to the level of evolved bioremediators. Here we address the evolution of phosphotriesterases (enzymes for hydrolysis of organophosphate pesticides or chemical warfare agents) in such a scenario: In a previous functional metagenomics screening we had identified a promiscuous phosphotriesterase activity of the alpha/beta-hydrolase P91, with an unexpected Cys-His-Asp catalytic triad as the active site motif. We now probe evolvability of P91 using ultrahigh-throughput screening in microfluidic droplets, and test for the first time whether the unique catalytic motif of a cysteine-containing triad can adapt to achieve rates that rival existing phosphotriesterases. These mechanistically distinct enzymes achieve their high rates based on catalysis involving a metal-ion cofactor. A focussed, combinatorial library of P91 (> 105 members) was screened on-chip in microfluidic droplets by quantification of the reaction product, fluorescein. Within only two rounds of evolution P91's phosphotriesterase activity was increased ~ 400-fold to a kcat/KM of ~ 10 6 M-1 s-1, matching the catalytic efficiencies of naturally evolved metal-dependent phosphotriesterases. In contrast to its homologue acetylcholinesterase that suffers suicide inhibition, P91 shows fast de-phosphorylation rates and is rate-limited by the formation of the covalent adduct rather than by its hydrolysis. Our analysis highlights how the combination of focussed, combinatorial libraries with the ultrahigh throughput of droplet microfluidics can be leveraged to identify and enhance mechanistic strategies that have not reached high efficiency in Nature, resulting in alternative reagents with a novel catalytic machinery.

Title:

Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvonen M, Morgavi DP, Janssen DB, Hollfelder F

Ref:

PubMed

Other Papers

Send your questions or comments to :Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.

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