(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Bacilli: NE > Bacillales: NE > Paenibacillaceae: NE > Paenibacillus: NE > Paenibacillus campinasensis: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Paenibacillus sp. xw-6-66: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKLWENIQQTPGYEPGEGAFVPEITPYLIEDGVVRGAVLVLPGGGYRLRA DHEGEPVAKWLNSIGLSAFVVHYRVSPYQHPYPLLDAKRAIRLVRYHAQE WNIDQNRIGILGFSAGGHLAACASTHFDKGDPEASDPVERESSRPDISVL CYPVISFKQFTHEGSVSHLLGDHPTEALRRELSGELAVQPNTPPAFLWHT ADDSAVPVENSLLYAGALSKHHIPFGLHVFPHGRHGLGLAEDVPDVSRWT SLCEEWLRTIGYLKE
Reference
Title: Identification and characterization of an acetyl esterase from Paenibacillus sp. XW-6-66 and its novel function in 7-aminocephalosporanic acid deacetylation Ding J, Zhou Y, Zhu H, Deng M, Long L, Yang Y, Wu Q, Huang Z Ref: Biotechnol Lett, 41:1059, 2019 : PubMed
OBJECTIVES: To obtain a new acetyl esterase from Paenibacillus sp. XW-6-66 and apply the enzyme to 7-aminocephalosporanic acid (7-ACA) deacetylation. RESULTS: The acetyl esterase AesZY was identified from Paenibacillus sp. XW-6-66, and its enzymatic properties were investigated. With the putative catalytic triad Ser114-Asp203-His235, AesZY belongs to the Acetyl esterase (Aes) family which is included in the alpha/beta hydrolase superfamily and contains the consensus Gly-X-Ser-X-Gly motif. The maximum activity of AesZY was detected at pH 8.0 and 40 degrees C. AesZY was stable at different pH values ranging from 5.0 to 12.0, and was tolerant to several metal ions. Furthermore, the deacetylation activity of AesZY toward 7-ACA was approximately 7.5 U/mg, and the Kcat/Km value was 2.04 s(-1) mM(-1). CONCLUSIONS: Our results demonstrate the characterization of a new acetyl esterase belonging to the Aes family with potential biotechnological applications.
