(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > FCB group: NE > Bacteroidetes/Chlorobi group: NE > Bacteroidetes: NE > Bacteroidia: NE > Bacteroidales: NE > Bacteroidaceae: NE > Bacteroides: NE > Bacteroides cellulosilyticus: NE > Bacteroides cellulosilyticus DSM 14838: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) uncultured Bacteroides sp.: N, E.
Bacteroides cellulosilyticus CL02T12C19: N, E.
Bacteroides cellulosilyticus: N, E.
Bacteroides cellulosilyticus CAG:158: N, E.
Bacteroides intestinalis DSM 17393: N, E.
Bacteroides intestinalis: N, E.
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MKLKSILYTTLIASLITGCAQKPKEKEATAPVSASTNILGISYPVVNPDL SVTVRVNAPDADSVKLDLMKKYPMTKNADGIWEVTSDPQVPGFHYYFLEI DGCSVADPSSELFYGCGRMSSGIEIPEEGVDYYLPQHVPHGEIRTQMYYS DITQAWRKCLVYTPAGYDENNTQKYPVLYLQHGSGEDETGWSNQGKADNI LDNLIASQKAVPMLVVMDRGYATDPKEKETGQKGRFNFNTFERVVINELI PMIDKNYRTLPDREHRAIAGLSMGGFQAVSIGLAHLDKFAHIGGFSGGGR MNSNELNTAYNGVFADAEAFNQKVKTLYISLGTEEAARFKNVTEFHDVLT QANINHIYYESPGTAHEWLTWRRSLHQFAGLIFK
Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.
