Gene_locus Report for: 9bace-b3c969

Bacteroides intestinalis DSM 17393 feruloyl esterase, Bacint_01033 isoamylase N-terminal domain protein

Other strains: Bacteroides intestinalis (DSM 17393; CAG:564); Bacteroides cellulosilyticus CL02T12C19; DSM 14838

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > FCB group: N E > Bacteroidetes/Chlorobi group: N E > Bacteroidetes: N E > Bacteroidia: N E > Bacteroidales: N E > Bacteroidaceae: N E > Bacteroides: N E > Bacteroides intestinalis: N E > Bacteroides intestinalis DSM 17393: N E

A85-EsteraseD-FGH : 9bace-b3che1Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
A85-Feruloyl-Esterase : 9bace-b3c9d0.2Bacteroides intestinalis DSM 17393 BiFae1B Putative uncharacterized protein, 9bace-b3c594Bacteroides intestinalis DSM 17393 Putative uncharacterized protein, 9bace-b3c974Bacteroides intestinalis DSM 17393 Putative uncharacterized protein BACINT_01038-CE6/CE1, 9bace-b3c975 Bacteroides intestinalis DSM 17393 BACINT_01039-CE1 Putative uncharacterized protein, 9bace-b3cet1 Bacteroides intestinalis DSM 17393 Bacint_04212 BiFae1A ferulic acid esterase.
A85-IroE-IroD-Fes-Yiel : 9bace-b3cb92Bacteroides intestinalis DSM 17393 Putative uncharacterized protein, 9bace-b3ci38Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
Abhydrolase_7 : 9bace-b3cg27Bacteroides intestinalis Putative uncharacterized protein, 9bace-b3cg28Bacteroides intestinalis, Putative uncharacterized protein.
Acetyl-esterase_deacetylase : 9bace-b3c7p2Bacteroides intestinalis DSM 17393 Acetyl xylan esterase (AXE1), 9bace-b3cg29Bacteroides intestinalis DSM 17393 Acetyl xylan esterase (AXE1).
Bacterial_EstLip_FamX : 9bace-b3cfy2Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
BD-FAE : 9bace-b3c976Bacteroides intestinalis esterase BACINT_01040, 9bace-b3caa7Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
BioG_Pimeloyl-ACP-methyl-esterase : 9bace-b3cav2Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
DPP4N_Peptidase_S9 : 9bace-b3c6j1Bacteroides intestinalis, Bacteroides cellulosilyticus, Putative uncharacterized protein, 9bace-b3c948Bacteroides intestinalis DSM 17393 Putative uncharacterized protein, 9bace-b3cg11Bacteroides intestinalis, Bacteroides cellulosilyticus, Putative uncharacterized protein.
Peptidase_S37 : 9bace-b3c8j8Bacteroides intestinalis DSM 17393 Putative uncharacterized protein.
PolyAspartate-hydrolase : 9bace-b3c5f6Bacteroides intestinalis DSM 17393 Bacteroides cellulosilyticus DSM 14838 Putative uncharacterized protein

Title: Degradation of complex arabinoxylans by human colonic Bacteroidetes
Pereira GV, Abdel-Hamid AM, Dutta S, D'Alessandro-Gabazza CN, Wefers D, Farris JA, Bajaj S, Wawrzak Z, Atomi H and Cann I <4 more author(s)> Pereira GV, Abdel-Hamid AM, Dutta S, D'Alessandro-Gabazza CN, Wefers D, Farris JA, Bajaj S, Wawrzak Z, Atomi H, Mackie RI, Gabazza EC, Shukla D, Koropatkin NM, Cann I (- 4)
Ref: Nat Commun, 12:459, 2021 : PubMed
Abstract


ESTHER: Pereira_2021_Nat.Commun_12_459
PubMedSearch: Pereira 2021 Nat.Commun 12 459
PubMedID: 33469030
Gene_locus related to this paper: 9bace-k9e1w0, 9bace-b3c976, 9bace-e2ncy6, 9bace-e2ncy7, 9bace-a0a380yku1, 9bace-b3c969, 9bace-b3c974, 9bace-b3c975

Abstract

Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.