Bacteroides eggerthii, Bifunctional GH43-CE, BACEGG_01304 only c-term 556-775 esterase
Comment
Bifunctional enzyme the N-term is GH43_C2 (not alpha/beta hydrolase) and esterase in C-term Hormone sensitive lipase like enzyme . Other strains: Bacteroides eggerthii 1_2_48FAA
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > FCB group: NE > Bacteroidetes/Chlorobi group: NE > Bacteroidetes: NE > Bacteroidia: NE > Bacteroidales: NE > Bacteroidaceae: NE > Bacteroides: NE > Bacteroides eggerthii: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Bacteroides eggerthii DSM 20697: N, E.
Bacteroides eggerthii 1_2_48FAA: N, E.
Molecular evidence
Database
No mutation 1 structure: 6MLY: Bifunctional GH43-CE Bacteroides eggerthii, BACEGG_01304 only c-term esterase No kinetic
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA RWEDICYADDKLEGHKLDIYLPDMDEPSYKVVVLIYGSAWFANNMKQAAF QVFGKSLLDKGFAVVSINHRSSGDAKFPAQINDVKAAIRFIRANAAKYKL DTSFIGITGFSSGGHLASLAGTTNGVKSYTIGAKTVDLEGNVGLYPSFSS RVDAVVNWFGPIDMTRMENCNTTKGANSPEAALIGGVPADNLDMLALLNP ITYIDKNDPKFIVIHGEADTVVPNCQSIFFSEALRAQGRLEEFISVPGGQ HGPVTFNENTLKKMIDFFAREAGI
Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.
Bacteroides eggerthii, Bifunctional GH43-CE, BACEGG_01304 only c-term 556-775 esterase
