(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Actinobacteria [phylum]: NE > Actinobacteria [class]: NE > Micromonosporales: NE > Micromonosporaceae: NE > Salinispora: NE > Salinispora pacifica: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRKVFSRDGTTIAYQRAGAGPPIVLLNGAFRDHTIFDALVPELTAHCTTY VYDRRGRGQSADSPRYAIEREIEDLAVVIEEAGGHAVVFAGSCGANLALE AALAGTPITKLALHEPYFRVGDHPRPPLDAADQLRALVADGQRGAAAEYF LNVLMDLPPTAIAQWRQGPLWATNEANAHTLAYDATLCGDYTLPADRLAA LRTPTLVVNSDGTSDWLRAAAQATAEALPNARGRELPGSWHRIEMEALGR VLVEFATT
The lomaiviticins are a family of cytotoxic marine natural products that have captured the attention of both synthetic and biological chemists due to their intricate molecular scaffolds and potent biological activities. Here we describe the identification of the gene cluster responsible for lomaiviticin biosynthesis in Salinispora pacifica strains DPJ-0016 and DPJ-0019 using a combination of molecular approaches and genome sequencing. The link between the lom gene cluster and lomaiviticin production was confirmed using bacterial genetics, and subsequent analysis and annotation of this cluster revealed the biosynthetic basis for the core polyketide scaffold. Additionally, we have used comparative genomics to identify candidate enzymes for several unusual tailoring events, including diazo formation and oxidative dimerization. These findings will allow further elucidation of the biosynthetic logic of lomaiviticin assembly and provide useful molecular tools for application in biocatalysis and synthetic biology.
