renre-luc
Renilla reniformis luciferase , luciferin 2-monooxygenase
Comment
Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of an ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>). This luciferase produces light with a wavelength of 480 nm. In presence of a green fluorescence protein (GFP) it produces a green fluorescence at 509 nm. It is different of most other luciferases which are not alpha Beta hydrolases
Relationship
Family : Haloalkane_dehalogenase-HLD2
Block : X
Position in NCBI Life Tree : Renilla reniformis
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Metazoa N E > Eumetazoa N E > Cnidaria N E > Anthozoa N E > Octocorallia N E > Pennatulacea N E > Sessiliflorae N E > Renillidae N E > Renilla N E > Renilla reniformis N EMolecular evidence
No mutation
2PSD - Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (diammonium)
2PSE - Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (KSCN)
2PSF - Crystal Structure of the Luciferase from Renilla Reniformis A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (thiomaltoside)
2PSH - Crystal Structure of the Luciferase from Renilla Reniformis T2A, K25A, A55T, C124A, S130A, K136R, A143M, M185V, M253L, E277A, S287L (PEG\/isopropanol)
2PSJ - Crystal Structure of the Luciferase from Renilla Reniformis T2A, A55T, C124A, S130A, K136R, A143M, M185V, M253L, S287L (PEG\/Isopropanol)
6YN2 - Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F\/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex)
7OMD - Crystal structure of azacoelenterazine-bound Renilla reniformis luciferase variant RLuc8-D162A
7OMO - Crystal structure of coelenteramine-bound Renilla reniformis luciferase RLuc8-D120A variant with fully hydrolyzed product
7OMR - Crystal structure of coelenteramide-bound Renilla reniformis luciferase RLuc8-D162A variant
No kinetic
No disease
Sequence
Peptide
MTSKVYDPEQ RKRMITGPQW WARCKQMNVL DSFINYYDSE KHAENAVIFL HGNAASSYLW RHVVPHIEPV ARCIIPDLIG MGKSGKSGNG SYRLLDHYKY LTAWFELLNL PKKIIFVGHD WGACLAFHYS YEHQDKIKAI VHAESVVDVI ESWDEWPDIE EDIALIKSEE GEKMVLENNF FVETMLPSKI MRKLEPEEFA AYLEPFKEKG EVRRPTLSWP REIPLVKGGK PDVVQIVRNY NAYLRASDDL PKMFIESDPG FFSNAIVEGA KKFPNTEFVK VKGLHFSQED APDEMGKYIK SFVERVLKNE Q
References (15)
| Title : Uncovering the role of sorbitol in Renilla luciferase kinetics: Insights from spectroscopic and molecular dynamics studies - Khoshnevisan_2024_Biochem.Biophys.Rep_37_101617 |
| Author(s) : Khoshnevisan G , Emamzadeh R , Nazari M , Oliayi M , Sariri R |
| Ref : Biochem Biophys Rep , 37 :101617 , 2024 |
| Abstract : |
| PubMedSearch : Khoshnevisan_2024_Biochem.Biophys.Rep_37_101617 |
| PubMedID: 38371529 |
| Gene_locus related to this paper: renre-luc |
| Title : Catalytic mechanism for Renilla-type luciferases - Schenkmayerova_2023_Nat.Catal_6_23 |
| Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M |
| Ref : Nature Catalysis , 6 :23 , 2023 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2023_Nat.Catal_6_23 |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc |
| Title : Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues - Gao_2023_ChemCatChem_15_e202300745 |
| Author(s) : Gao T , Damborsky J , Janin YL , Marek M |
| Ref : ChemCatChem , 15 :e202300745 , 2023 |
| Abstract : |
| PubMedSearch : Gao_2023_ChemCatChem_15_e202300745 |
| PubMedID: |
| Gene_locus related to this paper: renre-luc |
| Title : A catalytic mechanism for Renilla-type bioluminescence - Schenkmayerova_2022_Biorxiv__ |
| Author(s) : Schenkmayerova A , Toul M , Pluskal D , Baatallah R , Gagnot G , Pinto GP , Santana VT , Stuchla M , Neugebauer P , Chaiyen P , Damborsky J , Bednar D , Janin YL , Prokop Z , Marek M |
| Ref : Biorxiv , : , 2022 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2022_Biorxiv__ |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , renre-luc |
| Title : Engineering the protein dynamics of an ancestral luciferase - Schenkmayerova_2021_Nat.Commun_12_3616 |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Daniel Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Nat Commun , 12 :3616 , 2021 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2021_Nat.Commun_12_3616 |
| PubMedID: 34127663 |
| Gene_locus related to this paper: renre-luc |
| Title : Engineering Protein Dynamics of Ancestral Luciferase - Schenkmayerova_2020_Chemrxiv__ |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Chemrxiv , : , 2020 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2020_Chemrxiv__ |
| PubMedID: |
| Gene_locus related to this paper: renre-luc |
| Title : Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts - Chaloupkova_2019_ACS.Catal_9_48103 |
| Author(s) : Chaloupkova, R , Liskova V , Tool M , Markova K , Sebestova E , Hernychova L , Marek M , Pinto GP , Pluskal D , Waterman J , Prokop Z , Damborsky J |
| Ref : ACS Catal , 9 :4810 , 2019 |
| Abstract : |
| PubMedSearch : Chaloupkova_2019_ACS.Catal_9_48103 |
| PubMedID: |
| Gene_locus related to this paper: 9zzzz-AncHLDRLuc2 , 9zzzz-AncHLDRLuc , renre-luc |
| Title : Probing the emitter site of Renilla luciferase using small organic molecules\; an attempt to understand the molecular architecture of the emitter site - Salehi_2016_Int.J.Biol.Macromol_93_1253 |
| Author(s) : Salehi F , Emamzadeh R , Nazari M , Rasa SM |
| Ref : Int J Biol Macromol , 93 :1253 , 2016 |
| Abstract : |
| PubMedSearch : Salehi_2016_Int.J.Biol.Macromol_93_1253 |
| PubMedID: 27651278 |
| Gene_locus related to this paper: renre-luc |
| Title : Understanding the molecular behaviour of Renilla luciferase in imidazolium-based ionic liquids, a new model for the alpha\/beta fold collapse - Ghaedizadeh_2016_Biochem.Eng.J_105_505 |
| Author(s) : Ghaedizadeh S , Emamzadeh R , Nazari M , Rasa SMM , Zarkesh-Esfahani SH , Yousefi M |
| Ref : Biochem Eng J , 105 :505 , 2016 |
| Abstract : |
| PubMedSearch : Ghaedizadeh_2016_Biochem.Eng.J_105_505 |
| PubMedID: |
| Gene_locus related to this paper: renre-luc |
| Title : Structure-function studies on the active site of the coelenterazine-dependent luciferase from Renilla - Woo_2008_Protein.Sci_17_725 |
| Author(s) : Woo J , Howell MH , von Arnim AG |
| Ref : Protein Science , 17 :725 , 2008 |
| Abstract : |
| PubMedSearch : Woo_2008_Protein.Sci_17_725 |
| PubMedID: 18359861 |
| Gene_locus related to this paper: renre-luc |
| Title : Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis - Loening_2007_J.Mol.Biol_374_1017 |
| Author(s) : Loening AM , Fenn TD , Gambhir SS |
| Ref : Journal of Molecular Biology , 374 :1017 , 2007 |
| Abstract : |
| PubMedSearch : Loening_2007_J.Mol.Biol_374_1017 |
| PubMedID: 17980388 |
| Gene_locus related to this paper: renre-luc |
| Title : Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output - Loening_2006_Protein.Eng.Des.Sel_19_391 |
| Author(s) : Loening AM , Fenn TD , Wu AM , Gambhir SS |
| Ref : Protein Engineering Des Sel , 19 :391 , 2006 |
| Abstract : |
| PubMedSearch : Loening_2006_Protein.Eng.Des.Sel_19_391 |
| PubMedID: 16857694 |
| Gene_locus related to this paper: renre-luc |
| Title : Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors - Venisnik_2006_Protein.Eng.Des.Sel_19_453 |
| Author(s) : Venisnik KM , Olafsen T , Loening AM , Iyer M , Gambhir SS , Wu AM |
| Ref : Protein Engineering Des Sel , 19 :453 , 2006 |
| Abstract : |
| PubMedSearch : Venisnik_2006_Protein.Eng.Des.Sel_19_453 |
| PubMedID: 16882674 |
| Gene_locus related to this paper: renre-luc |
| Title : Isolation and expression of a cDNA encoding Renilla reniformis luciferase - Lorenz_1991_Proc.Natl.Acad.Sci.U.S.A_88_4438 |
| Author(s) : Lorenz WW , McCann RO , Longiaru M , Cormier MJ |
| Ref : Proceedings of the National Academy of Sciences of the United States of America , 88 :4438 , 1991 |
| Abstract : |
| PubMedSearch : Lorenz_1991_Proc.Natl.Acad.Sci.U.S.A_88_4438 |
| PubMedID: 1674607 |
| Gene_locus related to this paper: renre-luc |
| Title : Purification and properties of Renilla reniformis luciferase - Matthews_1977_Biochemistry_16_85 |
| Author(s) : Matthews JC , Hori K , Cormier MJ |
| Ref : Biochemistry , 16 :85 , 1977 |
| Abstract : |
| PubMedSearch : Matthews_1977_Biochemistry_16_85 |
| PubMedID: 12797 |
| Gene_locus related to this paper: renre-luc |